Improvement and evaluation of Aedes aegypti transgenic lines to control dengue transmission

• Grant number: 13/19921-9
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2015
• End date: November 30, 2017
• Field of knowledge: Biological Sciences - Parasitology - Entomology and Malacology of Parasites and Vectors
• Principal Investigator: Margareth de Lara Capurro-Guimarães
• Grantee: Margareth de Lara Capurro-Guimarães
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The aim of this project is based on the use of transgenic lines of Ae. aegypti, to be adding methodology of dengue control already used. The results showed that the strain OX513A reduction strategy is viable to reduce mosquitoes population. However, it is unique and can be improved. Our strategy is based on the creation and use of three strains of genetically modified Ae. aegypti. The first act in suppressing populations (male sterile) in association with the second strain that is genetically modified to produce only males (female sex reversion - GSS) and the third acting in introducing gene (Virus-lethal)In terms of innovation and improvement in population suppression, our strain have two points: the first is that for the production of male-sterile, tetracycline not be used in mass production (only in the colonies), thereby reducing the cost of production thus as the treatment of produced water. In addition to obtaining the GSS strain (males only) would not need to physically separate the males from the females and 100% of the production will be released, greatly improving the efficiency of the process. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

COSTA-DA-SILVA, ANDRE LUIS; IOSHINO, RAFAELLA SAYURI; PETERSEN, VIVIAN; LIMA, ANTONIO FERNANDO; CUNHA, MARIELTON DOS PASSOS; WILEY, MICHAEL R.; LADNER, JASON T.; PRIETO, KARLA; PALACIOS, GUSTAVO; COSTA, DANUZA DUARTE; et al. First report of naturally infected Aedes aegypti with chikungunya virus genotype ECSA in the Americas. PLoS Neglected Tropical Diseases, v. 11, n. 6, JUN 2017. (13/19921-9, 16/08204-2, 14/17766-9, 14/27172-9)

CARVALHO, DANILO O.; CHUFFI, SAMIRA; IOSHINO, RAFAELLA S.; MARQUES, ISABEL C. S.; FINI, REGINA; COSTA, MARIA KARINA; ARAUJO, HELENA R. C.; COSTA-DA-SILVA, ANDRE L.; KOJIN, BIANCA BURINI; CAPURRO, MARGARETH L.. Mosquito pornoscopy: Observation and interruption of Aedes aegypti copulation to determine female polyandric event and mixed progeny (vol 13, e0193164, 2018). PLoS One, v. 13, n. 12, p. 1-pg., 2018-12-10. (13/19921-9)

COSTA-DA-SILVA, ANDRE LUIS; IOSHINO, RAFAELLA SAYURI; CORREA DE ARAUJO, HELENA ROCHA; KOJIN, BIANCA BURINI; DE ANDRADE ZANOTTO, PAOLO MARINHO; LEAL OLIVEIRA, DANIELLE BRUNA; MELO, STELLA REZENDE; DURIGON, EDISON LUIZ; CAPURRO, MARGARETH LARA. Laboratory strains of Aedes aegypti are competent to Brazilian Zika virus. PLoS One, v. 12, n. 2, FEB 10 2017. (13/19921-9, 14/17766-9, 13/16609-4)

IOSHINO, RAFAELLA SAYURI; CARVALHO, DANILO OLIVEIRA; SANTOS MARQUES, ISABEL CRISTINA; FERNANDES, EDIANE SARAIVA; CAPURRO, MARGARETH LARA; COSTA-DA-SILVA, ANDRE LUIS. Oviplate: A Convenient and Space-Saving Method to Perform Individual Oviposition Assays in Aedes aegypti. INSECTS, v. 9, n. 3, SEP 2018. (13/19921-9, 14/17766-9)