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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Expression of genes related to mitochondrial function in Nellore cattle divergently ranked on residual feed intake

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Autor(es):
Simielli Fonseca, Larissa Fernanda [1] ; Jovino Gimenez, Daniele Fernanda [1] ; Zerlotti Mercadante, Maria Eugenia [2, 3] ; Martins Bonilha, Sarah Figueiredo [2, 3] ; Ferro, Jesus Aparecido [4, 3] ; Baldi, Fernando [1, 3] ; Pablos de Souza, Fabio Ricardo [5] ; de Albuquerque, Lucia Galvao [1, 3]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] UNESP Univ Estadual Paulista, Dept Zootecnia, Fac Ciencias Agr & Vet, BR-14884900 Jaboticabal, SP - Brazil
[2] Ctr Pesquisa Pecuaria Corte, Inst Zootecnia, BR-14160000 Sertaozinho, SP - Brazil
[3] Conselho Nacl Desenvolvimento Cient & Tecnol CNPq, BR-71605001 Lago Sul, DF - Brazil
[4] UNESP Univ Estadual Paulista, Dept Tecnol, Fac Ciencias Agr & Vet, BR-14884900 Jaboticabal, SP - Brazil
[5] Univ Fed Pelotas, Dept Zool & Genet, BR-96010900 Pelotas, RS - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: MOLECULAR BIOLOGY REPORTS; v. 42, n. 2, p. 559-565, FEB 2015.
Citações Web of Science: 4
Resumo

Several measures have been proposed to investigate and improve feed efficiency in cattle. One of the most commonly used measure of feed efficiency is residual feed intake (RFI), which is estimated as the difference between actual feed intake and expected feed intake based on the animal's average live weight. This measure permits to identify and select the most efficient animals without selecting for higher mature weight. Mitochondrial function has been indicated as a major factor that influences RFI. The analysis of genes involved in mitochondrial function is therefore an alternative to identify molecular markers associated with higher feed efficiency. This study analyzed the expression of PGC1 alpha, TFAM, UCP2 and UCP3 genes by quantitative real-time PCR in liver and muscle tissues of two groups of Nellore cattle divergently ranked on RFI values in order to evaluate the relationship of these genes with RFI. In liver tissue, higher expression of TFAM and UCP2 genes was observed in the negative RFI group. Expression of PGC1 alpha gene did not differ significantly between the two groups, whereas UCP3 gene was not expressed in liver tissue. In muscle tissue, higher expression of TFAM gene was observed in the positive RFI group. Expression of PGC1 alpha, UCP2 and UCP3 genes did not differ significantly between the two groups. These results suggest the use of TFAM and UCP2 as possible candidate gene markers in breeding programs designed to increase the feed efficiency of Nellore cattle. (AU)

Processo FAPESP: 09/16118-5 - Ferramentas genômicas no melhoramento genético de características de importância econômica direta em bovinos da raça Nelore
Beneficiário:Lucia Galvão de Albuquerque
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 10/13502-6 - Expressão de genes relacionados à função mitocondrial em bovinos Nelore extremos para consumo alimentar residual
Beneficiário:Larissa Fernanda Simielli Fonseca
Linha de fomento: Bolsas no Brasil - Mestrado