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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Encapsulation of curcumin in polymeric nanoparticles for antimicrobial Photodynamic Therapy

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Autor(es):
Trigo Gutierrez, Jeffersson Krishan [1] ; Zanatta, Gabriela Cristina [1] ; Mira Ortega, Ana Laura [1] ; Cuba Balastegui, Maria Isabella [1] ; Sanita, Paula Volpato [1] ; Pavarina, Ana Claudia [1] ; Barbugli, Paula Aboud [1] ; de Oliveira Mima, Ewerton Garcia [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ, UNESP, Sch Dent, Dept Dent Mat & Prosthodont, Araraquara, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 12, n. 11 NOV 6 2017.
Citações Web of Science: 0
Resumo

Curcumin (CUR) has been used as photosensitizer in antimicrobial Photodynamic Therapy (aPDT). However its poor water solubility, instability, and scarce bioavalibility hinder its in vivo application. The aim of this study was to synthesize curcumin in polymeric nanoparticles (NP) and to evaluate their antimicrobial photodynamic effect and cytoxicity. CUR in anionic and cationic NP was synthesized using polylactic acid and dextran sulfate by the nanoprecipitation method. For cationic NP, cetyltrimethylammonium bromide was added. CUR-NP were characterized by physicochemical properties, photodegradation, encapsulation efficiency and release of curcumin from nanoparticles. CUR-NP was compared with free CUR in 10% dimethyl sulfoxide (DMSO) as a photosensitizer for aPDT against planktonic and biofilms (mono-, dual-and triple-species) cultures of Streptococcus mutans, Candida albicans and Methicillin-Resistant Staphylococcus aureus. The cytotoxicity effect of formulations was evaluated on keratinocytes. Data were analysed by parametric (ANOVA) and non-parametric (Kruskal-Wallis) tests (alpha = 0.05). CUR-NP showed alteration in the physicochemical properties along time, photodegradation similar to free curcumin, encapsulation efficiency up to 67%, and 96% of release after 48h. After aPDT planktonic cultures showed reductions from 0.78 loglo to complete eradication, while biofilms showed no antimicrobial effect or reductions up to 4.44 loglo. Anionic CUR-NP showed reduced photoinactivation of biofilms. Cationic CUR-NP showed microbicidal effect even in absence of light. Anionic formulations showed no cytotoxic effect compared with free CUR and cationic CURNP and NP. The synthesized formulations improved the water solubility of CUR, showed higher antimicrobial photodynamic effect for planktonic cultures than for biofilms, and the encapsulation of CUR in anionic NP reduced the cytotoxicity of 10% DMSO used for free CUR. (AU)

Processo FAPESP: 13/23165-5 - Síntese, caracterização e efeitos fotodinâmicos de curcumina encapsulada em nanopartículas poliméricas em biofilmes in vitro de Candida Albicans e em modelo murino de candidose bucal
Beneficiário:Ewerton Garcia de Oliveira Mima
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 14/18345-7 - Efeitos fotodinâmicos de curcumina encapsulada em nanopartículas poliméricas em biofilme multiespécies de Staphylococcus aureus resistente à meticilina e Candida Albicans
Beneficiário:Ana Laura Mira Ortega
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 14/20119-5 - Síntese, caracterização e efeitos fotodinâmicos de curcumina encapsulada em nanopartículas poliméricas em biofilmes in vitro de Candida Albicans e em modelo murino de candidose bucal
Beneficiário:Geisiane Helena Gomes Bueno
Linha de fomento: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
Processo FAPESP: 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica
Beneficiário:Vanderlei Salvador Bagnato
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs