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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Portable fluorescence lifetime spectroscopy system for in-situ interrogation of biological tissues

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Autor(es):
Nogueira, Marcelo Saito [1] ; Cosci, Alessandro [1] ; Teixeira Rosa, Ramon Gabriel [1] ; Salvio, Ana Gabriela [2] ; Pratavieira, Sebastiao [1] ; Kurachi, Cristina [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sao Carlos Inst Phys, Sao Carlos, SP - Brazil
[2] Amaral Carvalho Hosp, Jahu, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Journal of Biomedical Optics; v. 22, n. 12 DEC 2017.
Citações Web of Science: 0
Resumo

Fluorescence spectroscopy and lifetime techniques are potential methods for optical diagnosis and characterization of biological tissues with an in-situ, fast, and noninvasive interrogation. Several diseases may be diagnosed due to differences in the fluorescence spectra of targeted fluorophores, when, these spectra are similar, considering steady-state fluorescence, others may be detected by monitoring their fluorescence lifetime. Despite this complementarity, most of the current fluorescence lifetime systems are not robust and portable, and not being feasible for clinical applications. We describe the assembly of a fluorescence lifetime spectroscopy system in a suitcase, its characterization, and validation with clinical measurements of skin lesions. The assembled system is all encased and robust, maintaining its mechanical, electrical, and optical stability during transportation, and is feasible for clinical measurements. The instrument response function measured was about 300 ps, and the system is properly calibrated. At the clinical study, the system showed to be reliable, and the achieved spectroscopy results support its potential use as an auxiliary tool for skin diagnostics. (c) 2017 Society of Photo-Optical Instrumentation Engineers (SPIE) (AU)

Processo FAPESP: 14/16154-0 - Espectroscopia de fluorescência para diagnóstico de lesões de pele clinicamente semelhantes
Beneficiário:Marcelo Saito Nogueira
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica
Beneficiário:Vanderlei Salvador Bagnato
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
Processo FAPESP: 14/50857-8 - INCT 2014 - de Óptica Básica e Aplicada às Ciências da Vida
Beneficiário:Vanderlei Salvador Bagnato
Linha de fomento: Auxílio à Pesquisa - Temático