Impact of gastroplasty on salivary composition and cytomorphology of oral mucosa: follow-up three and six months

• Grant number: 17/26400-6
• Support Opportunities: Regular Research Grants
• Duration: June 01, 2018 - November 30, 2020
• Field of knowledge: Biological Sciences - Physiology - Physiology of Organs and Systems
• Principal Investigator: Paula Midori Castelo Ferrua
• Grantee: Paula Midori Castelo Ferrua
• Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil

Abstract

The aim of this study is to evaluate the impact of gastroplasty in salivary physicochemical and microbiological composition, concentration of sulfurated compounds volatile related to halitosis and cytomorphology of the oral mucosa of 60 obese adults (18-40 years of age) undergoing gastroplasty (Capella technique) evaluated before and 3 and 6 months after surgery. Thirty normal weight (body mass index between 18.5 and 24.9 kg/m2), matched for sex and age, will be part of the control group, which will also be evaluated three times. The physical examination includes measurements of height, weight, fat mass and skeletal muscle mass through a digital stadiometer and bioelectrical impedance analysis. DMFT and Community Periodontal Index will verify the oral health status. The self- perception of oral health- related quality of life will be verify using OHIP-14 questionnaire. Salivary amylase, total protein, calcium, urea, uric acid, phosphorus, 25-hidroxivitamin D2 and 25 hidroxivitamin D3 concentrations will be determined in stimulated and unstimulated saliva by automated technique (colorimetry or HPLC). ELISA assays will analyze salivary cytokines. Salivary DNA samples will be purified and subjected to quantitative PCR reactions (qPCR) in order to determine the proportion of Selenomonas noxia, Bifidobacteria, Streptococcus mutans and Prevotella intermedia in relation to the total bacterial load. Micronucleus test will analyze oral mucosa cells. The description of the study variables and their correlation will be analyzed using descriptive statistics, normality test and correlation tests (Pearson or Spearman). Principal component analysis will verify the salivary characteristics of the patients at baseline and Two-way ANOVA and Tukey pot-test will be used for comparisons between groups. Two-way ANOVA repeated measures will verify the changes resulting from bariatric surgery (± = 0.05). (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LOURO, TERESA; SIMOES, CARLA; PENETRA, MARIA JOAO; CARREIRA, LAURA; CASTELO, PAULA MIDORI; LUIS, HENRIQUE; MOREIRA, PEDRO; LAMY, ELSA. Relationship between Mediterranean Diet Adherence and Saliva Composition. NUTRIENTS, v. 13, n. 4, APR 2021. (17/26400-6)

ANDRADE, ERIC FRANCELINO; PEREIRA, LUCIANO JOSE; LUIZ DE OLIVEIRA, ANA PAULA; ORLANDO, DEBORA RIBEIRO; GALDINO ALVES, DEBORA ALMEIDA; GUILARDUCCI, JANINA DE SALES; CASTELO, PAULA MIDORI. erceived fear of COVID-19 infection according to sex, age and occupational risk using the Brazilian version of the Fear of COVID-19 Scal. DEATH STUDIES, v. 46, n. 3, AUG 2020. (17/26400-6)

MARQUEZIN, MARIA CAROLINA SALOME; CHAVES-JUNIOR, SAMUEL DE CARVALHO; RASERA, JR., IRINEU; PACHECO, ELISANE ROSSIN PESSOTTI; GAVIAO, MARIA BEATRIZ DUARTE; LAMY, ELSA; CASTELO, PAULA MIDORI. Oral Health and Nutritional Characteristics of Adults With Morbid Obesity: A Multivariate Analysis. FRONTIERS IN NUTRITION, v. 7, NOV 20 2020. (17/26400-6, 16/10940-9)

ROSAR, JOAO VICENTE; SALOME MARQUEZIN, MARIA CAROLINA; PIZZOLATO, AIANNE SOUTO; KOBAYASHI, FERNANDA YUKIE; BUSSADORI, SANDRA KALIL; PEREIRA, LUCIANO JOSE; CASTELO, PAULA MIDORI. Identifying predictive factors for sleep bruxism severity using clinical and polysomnographic parameters: a principal component analysis. JOURNAL OF CLINICAL SLEEP MEDICINE, v. 17, n. 5, p. 949-956, MAY 1 2021. (17/26400-6, 16/10940-9)

ANDRADE, ERIC FRANCELINO; PEREIRA, LUCIANO JOSE; ORLANDO, DEBORA RIBEIRO; DIAS PEIXOTO, MARCO FABRICIO; LEE, SHERMAN A.; CASTELO, PAULA MIDORI. Validation of the Brazilian Portuguese version of the Obsession with COVID-19 Scale (BP-OCS) using a large University Sample in Brazil. DEATH STUDIES, JAN 2021. (17/26400-6)

ARAUJO, DARLLE SANTOS; KLEIN, MARLISE INEZ; SCUDINE, KELLY GUEDES DE OLIVEIRA; DE SALES LEITE, LUANA; PARISOTTO, THAIS M.; FERREIRA, CAROLINE MARCANTONIO; FONSECA, FERNANDO LUIZ AFFONSO; PEREZ, MATHEUS MOREIRA; CASTELO, PAULA MIDORI. Salivary Microbiological and Gingival Health Status Evaluation of Adolescents With Overweight and Obesity: A Cluster Analysis. FRONTIERS IN PEDIATRICS, v. 8, JUL 31 2020. (14/24804-4, 17/26400-6)

AZZI, DIANA VILELA; MELO, JULIA; CAMPOS NETO, ARMINDO DE ARRUDA; CASTELO, PAULA MIDORI; ANDRADE, ERIC FRANCELINO; PEREIRA, LUCIANO JOSE. uality of life, physical activity and burnout syndrome during online learning period in Brazilian university students during the COVID-19 pandemic: a cluster analysi. PSYCHOLOGY HEALTH & MEDICINE, v. 27, n. 2, JUL 2021. (17/26400-6)

ARAUJO, DARLLE SANTOS; DE OLIVEIRA SCUDINE, KELLY GUEDES; PEDRONI-PEREIRA, ALINE; DUARTE GAVIAO, MARIA BEATRIZ; PEREIRA, EDIMAR CRISTIANO; AFFONSO FONSECA, FERNANDO LUIZ; CASTELO, PAULA MIDORI. Salivary uric acid is a predictive marker of body fat percentage in adolescents. Nutrition Research, v. 74, p. 62-70, FEB 2020. (14/24804-4, 17/26400-6)

FERREIRA, CLAUDIA LUCIA PIMENTA; CASTELO, PAULA MIDORI; ZANATO, LUCIANA ESCANOELA; POYARES, DALVA; TUFIK, SERGIO; BOMMARITO, SILVANA. Relation between oro-facial thermographic findings and myofunctional characteristics in patients with obstructive sleep apnoea. Journal of Oral Rehabilitation, MAR 2021. (17/26400-6)