TREATMENT OF BREAST CANCER IN ANIMAL MODEL VIA PHOTODYNAMIC THERAPY ASSOCIATED WITH BIFUNCTIONAL NANOprobes

• Grant number: 17/07519-2
• Support Opportunities: Regular Research Grants
• Duration: June 01, 2018 - November 30, 2020
• Field of knowledge: Physical Sciences and Mathematics - Physics - Condensed Matter Physics
• Principal Investigator: Leandro José Raniero
• Grantee: Leandro José Raniero
• Host Institution: Instituto de Pesquisa e Desenvolvimento (IP&D). Universidade do Vale do Paraíba (UNIVAP). São José dos Campos , SP, Brazil
• Associated researchers: Denise Maria Zezell ; Ivone Regina de Oliveira ; Maiara Lima Castilho ; Patrícia Marcondes dos Santos ; Renata de Azevedo Canevari

Abstract

Cancer is a complex disease, triggered by many factors, health systems and lifestyle, such as eating habits, smoking, sedentary lifestyle among others, and may be derived from genetic mutations inherent in each individual, becoming a Public health problem. Conventional treatments are aggressive and on several occasions with unsatisfactory prognosis, which may be related to clinical heterogeneity of tumors, among others, the need for improvement / development of new methods is evident. Photodynamic Therapy (PDT) is an effective method for the treatment of various types of cancer by the use of a radiation for the activation of a photosensitizing agent (drug) in the presence of molecular oxygen. Unlike chemotherapy and radiotherapy, PDT shows good results without cumulative toxicity, and can be used even in immunocompromised patients. In PDT, a radiation photosensitizing assay at an appropriate wavelength, results in a formation of reactive oxygen species, such as singlet oxygen, promoting cell death. Although this drug is selective, accumulating in less concentration in healthy cells, increased drug uptake into cells may help with the efficiency of PDT. Aiming at the need for new treatments for breast cancer, less invasive, and with collateral side effects, the present project proposes to evaluate the antitumor and toxicological action of PDT using bifunctional nanoprobes in an animal model. Thus, gold nanoparticles with active targeting, using the Epidermal Growth Factor (EGF) protein and Chlorine e6 transporters, use in PDT as an alternative method of treatment against breast cancer. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CASTILHO, MAIARA L.; JESUS, VIVIANE P. S.; VIEIRA, PAULA F. A.; HEWITT, KEVIN C.; RANIERO, L.. Chlorin e6-EGF conjugated gold nanoparticles as a nanomedicine based therapeutic agent for triple negative breast cancer. Photodiagnosis and Photodynamic Therapy, v. 33, MAR 2021. (17/07519-2)

VIEIRA, PAULA; JESUS, VIVIANE; CANDIDO, MARCELA APARECIDA; PACHECO-SOARES, CRISTINA; CASTILHO, MAIARA; RANIERO, LEANDRO. Specific nanomarkers fluorescence: in vitro analysis for EGFR overexpressed cells in triple-negative breast cancer and malignant glioblastoma. Photodiagnosis and Photodynamic Therapy, v. 39, p. 7-pg., 2022-07-27. (17/07519-2)

ANDRADE, MAIRA FRANCO; SARDO, ARIANE VENZON NAIA; BENETTI, CAROLINA; SICCHIERI, LETICIA BONFANTE; CORREA, LUCIANA; ZEZELL, DENISE MARIA. Comparison of Two Light Wavelengths (lambda = 660 nm and lambda = 780 nm) in the Repair Process of Oral Mucositis Induced by Ionizing Radiation: Clinical and Microscopic Evaluations in Rats. PHOTONICS, v. 10, n. 1, p. 13-pg., 2023-01-01. (17/07519-2, 17/50332-0, 13/26113-6)

JESUS, VIVIANE PAULA DOS SANTOS; VIEIRA, PAULA FONSECA ANTUNES; CINTRA, RICARDO CESAR; 'ANNA, LUCIANA BARROS SANT; ZEZELL, DENISE MARIA; CASTILHO, MAIARA LIMA; RANIERO, LEANDRO. Triple-negative breast cancer treatment in xenograft models by bifunctional nanoprobes combined to photodynamic therapy. Photodiagnosis and Photodynamic Therapy, v. 38, p. 9-pg., 2022-03-25. (17/07519-2)