The liver of anuran amphibians: morphology compared between 10 species and action of benzopyrene in the melanomacrophages of Physalaemus cuvieri

• Grant number: 18/01078-7
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2018
• End date: November 30, 2020
• Field of knowledge: Biological Sciences - Zoology - Morphology of Recent Groups
• Principal Investigator: Classius de Oliveira
• Grantee: Classius de Oliveira
• Host Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil
• Associated researchers: Carlos Eurico dos Santos Fernandes ; Lia Raquel de Souza Santos ; Lilian Franco Belussi ; Rhayane Alves de Assis ; Taynara Ribeiro Farias Leão
• Associated scholarship(s): 18/10399-1 - Pigment composition of hepatic melanomacrophages in 15 species of anurans, BP.TT

Abstract

Anurans have pigment cells (melanocytes and melanomacrophages) in different organs and membranes, which have melanin. The visceral pigmentation can be related to residual melanin accumulation, may have a role in the immune system, antioxidant functions, and protect the tissue. One of the objectives of this study is to describe the morphometric characteristics of the hepatic melanomacrophages (MMs) and characterize the liver histology in ten species of anurans. Data analysis includes morphological and stereological analyses, quantification of melanin, hemosiderin and lipofuscin. The other purpose, according to the hypothesis that the Benzo[alpha]pyrene (BaP) is a hepatotoxic contaminant will be: to evaluate the dispersion of the melanin area of MMs (quantify the melanin production); to evaluate phagocytic activity of MMs (BaP changes in the cytoskeleton); to evaluate genotoxic effects. For the histological analyzes the procedures for inclusion in historesin and light microscopy analysis of the area of melanin and phagocytosis will be followed. The amount of melanin will be measured by Elisa. For microtubules, pellets with MMs will be analyzed (by fluorescence microscope). For nuclear abnormalities, the blood will be extracted and the procedures for analysis will be followed. These morphometric characteristics of liver tissue and properties of the MMs can be used as functional biomarkers for the assessment of anurans' health and to test the functions of visceral pigmentation related to chemical pollutant factors. (AU)

Scientific publications

(14)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ASSIS, RHAYANE ALVES; REZENDE, WADSON RODRIGUES; DOS SANTOS, CIRLEY GOMES ARAUJO; BENVINDO-SOUZA, MARCELINO; AMORIM, NATHAN PEREIRA LIMA; BORGES, RINNEU ELIAS; FRANCO-BELUSSI, LILIAN; DE OLIVEIRA, CLASSIUS; DE SOUZA SANTOS, LIA RAQUEL. Habitat differences affect the nuclear morphology of the erythrocytes and the hepatic melanin in Leptodactylus fuscus (Anura) in the Brazilian Cerrado savanna. Environmental Science and Pollution Research, JUN 2021. (18/01078-7)

FANALI, LARA ZACARI; DE OLIVEIRA, CLASSIUS; STURVE, JOACHIM. Enzymatic, morphological, and genotoxic effects of benzo[a]pyrene in rainbow trout (Oncorhynchus mykiss). Environmental Science and Pollution Research, v. 28, n. 38, p. 53926-53935, OCT 2021. (18/07989-1, 13/02067-5, 18/01078-7)

FANALI, LARA ZACARI; STURVE, JOACHIM; DE OLIVEIRA, CLASSIUS. xposure of Physalaemus cuvieri (Anura) to benzo[a]pyrene and alpha-naphthoflavone: Morphofunctional effects on hepatic melanomacrophages and erythrocytes abnormalitie. Environmental Pollution, v. 285, SEP 15 2021. (18/01078-7, 17/07971-2, 13/02067-5)

DE LACERDA VALVERDE, BRUNO SERRA; UTSUNOMIYA, HEIDI SAMANTHA MORAES; DOS SANTOS CARVALHO, CLEONI; FRANCO-BELUSSI, LILIAN; DE OLIVEIRA, CLASSIUS. Response of hepatic biomarkers in Physalaemus nattereri (Anura) to different benzo(alpha)pyrene exposure routes. ECOTOXICOLOGY, v. 31, n. 3, p. 8-pg., 2022-02-18. (18/01078-7, 17/23781-9)

REZENDE, WADSON RODRIGUES; DE SOUZA SANTOS, LIA RAQUEL; FRANCO-BELUSSI, LILIAN; DE OLIVEIRA, CLASSIUS. Testicular morphometric changes in neotropical anurans from agroecosystems. Environmental Pollution, v. 271, FEB 15 2021. (18/01078-7, 15/12006-9)

ASSIS, RHAYANE ALVES; REZENDE, WADSON RODRIGUES; DOS SANTOS, CIRLEY GOMES ARAUJO; BENVINDO-SOUZA, MARCELINO; AMORIM, NATHAN PEREIRA LIMA; BORGES, RINNEU ELIAS; FRANCO-BELUSSI, LILIAN; DE OLIVEIRA, CLASSIUS; DE SOUZA SANTOS, LIA RAQUEL. Habitat differences affect the nuclear morphology of the erythrocytes and the hepatic melanin in Leptodactylus fuscus (Anura) in the Brazilian Cerrado savanna. Environmental Science and Pollution Research, v. 28, n. 43, p. 60741-60752, NOV 2021. (18/01078-7)

DE GREGORIO, LARA SALGUEIRO; FRANCO-BELUSSI, LILIAN; GOLDBERG, JAVIER; DE OLIVEIRA, CLASSIUS. Nonylphenol and cyproterone acetate effects in the liver and gonads of Lithobates catesbeianus (Anura) tadpoles and juveniles. Environmental Science and Pollution Research, JUL 2021. (18/01078-7, 15/12006-9, 14/00946-4)

FANALI, LARA ZACARI; DE OLIVEIRA, CLASSIUS; STURVE, JOACHIM. Enzymatic, morphological, and genotoxic effects of benzo[a]pyrene in rainbow trout (Oncorhynchus mykiss). Environmental Science and Pollution Research, MAY 2021. (18/01078-7, 13/02067-5, 18/07989-1)

GREGORIO, L. S.; FRANCO-BELUSSI, L.; DE OLIVEIRA, C.. Genotoxic effects of 4-nonylphenol and Cyproterone Acetate on Rana catesbeiana (anura) tadpoles and juveniles. Environmental Pollution, v. 251, p. 879-884, AUG 2019. (14/00946-4, 13/02067-5, 18/01078-7)

LEAO, TAYNARA; SIQUEIRA, MAYARA; MARCONDES, SANDRIELY; FRANCO-BELUSSI, LILIAN; DE OLIVEIRA, CLASSIUS; FERNANDES, CARLOS E.. Comparative liver morphology associated with the hepatosomatic index in five Neotropical anuran species. Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology, v. 304, n. 4, OCT 2020. (18/01078-7)

FRANCO-BELUSSI, LILIAN; PROVETE, DIOGO B.; BORGES, RINNEU E.; DE OLIVEIRA, CLASSIUS; SANTOS, LIA RAQUEL S.. Idiosyncratic liver pigment alterations of five frog species in response to contrasting land use patterns in the Brazilian Cerrado. PeerJ, v. 8, AUG 26 2020. (14/00946-4, 13/02067-5, 18/01078-7)

DE GREGORIO, LARA S.; FRANCO-BELUSSI, LILIAN; DE OLIVEIRA, CLASSIUS. Leukocyte profile of tadpoles and juveniles of Lithobates catesbeianus Shaw, 1802 (Anura) and the effects of nonylphenol and cyproterone acetate. South American Journal of Herpetology, v. 20, n. 1, p. 75-82, AUG 2021. (14/00946-4, 18/01078-7)

SANTOS, ARLETO T.; VALVERDE, BRUNO S. L.; DE OLIVEIRA, CLASSIUS; FRANCO-BELUSSI, LILIAN. Genotoxic and melanic alterations in Lithobates catesbeianus (anura) tadpoles exposed to fipronil insecticide. Environmental Science and Pollution Research, v. 28, n. 16, p. 20072-20081, APR 2021. (18/01078-7)

DE GREGORIO, LARA SALGUEIRO; FRANCO-BELUSSI, LILIAN; GOLDBERG, JAVIER; DE OLIVEIRA, CLASSIUS. Nonylphenol and cyproterone acetate effects in the liver and gonads of Lithobates catesbeianus (Anura) tadpoles and juveniles. Environmental Science and Pollution Research, v. 28, n. 44, p. 62593-62604, NOV 2021. (18/01078-7, 15/12006-9, 14/00946-4)