Biodiversity of endophytic microorganisms and their biotechnological potential

• Grant number: 99/09177-1
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
• Start date: January 01, 2001
• End date: May 31, 2005
• Field of knowledge: Biological Sciences - Microbiology
• Principal Investigator: Itamar Soares de Melo
• Grantee: Itamar Soares de Melo
• Host Institution: Embrapa Meio-Ambiente. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA). Ministério da Agricultura, Pecuária e Abastecimento (Brasil). Jaguariúna , SP, Brazil
• Pesquisadores principais: João Lúcio de Azevedo
• Associated scholarship(s): 04/01741-5 - Selection of endophytic microorganisms as agents of biocontrol of coffee rust, BP.IC; 03/05759-3 - Endophytic microorganisms as agents of biocontrol of coffee rust (Hemileia vastatrix) and as growth promoters, BP.PD; 03/06346-4 - Biodiversity of endophytic microorganisms and their biotechnological potential, BP.TT; 01/07122-7 - Diversity of endophytic bacteria of coffee plants (Coffea arabica e Coffea robusta), BP.MS; 01/06443-4 - Endophytic microorganisms: cloning and characterization of genes of hydrolases and their proteins, BP.PD

Abstract

Endophytic bacteria and fungi are defined as organisms that live in association with plants for most if not all of their life cycles. They live withim the intercellular spaces of plants, where they live off apoplastic nutrients. Some fungi and bacteria can be isolated from a great variety of host plant families while growing under different ecological and geographical conditions. Other endophytes are apparently restricted to host species that occur within a particular plant family. Endophytic fungi and bacteria may confer benefits to the plant, and the benefits may be reciprocal, resulting in an enhanced symbiotic system for specific plant characteristics. Therefore, the use of endophytic bacteria and fungi opens up new areas of biothechnological exploitations, which drive the necessity to isolate and culture these organisms. Endophytes are used for biological control of various plant diseases, for enhanced agronomic plant characteristic such as increased drought tolerances and nitrogen efficiency, for bioherbicides, and for pharmaceutical agents. Because these microorganisms are usually obligated biotrophic parasites, their culture, especially for fungi, if ever achieved, may be difficult and slow. The biochemical versatility and diversity of endophytes represent an enormous variety of genes that are still unknown. More and more useful gene functions are being discovered, particularly for environmental remediation and industrial processes. Endophytics also provide numerous antimicrobial compounds; for instances, the fungus Pestalotiopsis microspora, and endophytic of Hymalayan yew, Taxus walkchiana produces taxol. This compound is now marketed as an anticancer drug (Strobel et aI., Microbiology, v.142, p. 435-440,1996). This project proposes to study the biological diversity of endophytic microorganisms (considering both number of species and the partition of abundance) from important plant species growing in São Paulo State, such as: maize, soybeans, citros, coffee, cassava, Brachiaria and Dicksonia (xaxim); to study the molecular diversity (molecular systematics) present within a species or genus based comparative sequencing of the rRNAs of endophytic microorganisms; to determine the chemical diversity, mainly of secondary metabolites-"antibiotics"; to assess the potential of endophytes in the biological controlof most important pathogens of the host plants (rust in coffee, Xyllela in citros, Phytophthora in cassava, damping-off in soybeans, Phythium aphanidermatum in maize). Also, it is objective of this project to study best methods of preserving representative genera and specific groups and to organize a culture collection of ali identified endophytics. Culture collections represent valuable resources of microbial germoplasm. Our objective is to make this collection available for distribution and utilization. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PEREIRA DE MELO, FLAVIA MANDOLESI; FIORE, MARLI FATIMA; BERALDO DE MORAES, LUIZ ALBERTO; SILVA-STENICO, MARIA ESTELA; SCRAMIN, SHIRLEI; TEIXEIRA, MANOEL DE ARAUJO; DE MELO, ITAMAR SOARES. Composto antifúngico produzido pelo endófito de mandioca Bacillus pumilus MAIIIM4a. Scientia Agricola, v. 66, n. 5, p. 583-592, 2009. (99/09177-1)

SILVA, HARLLEN S. A.; TOZZI, JOAO P. L.; TERRASAN, CESAR R. F.; BETTIOL, WAGNER. Endophytic microorganisms from coffee tissues as plant growth promoters and biocontrol agents of coffee leaf rust. BIOLOGICAL CONTROL, v. 63, n. 1, p. 62-67, OCT 2012. (99/09177-1)

PEREIRA DE MELO, FLAVIA MANDOLESI; FIORE, MARLI FATIMA; BERALDO DE MORAES, LUIZ ALBERTO; SILVA-STENICO, MARIA ESTELA; SCRAMIN, SHIRLEI; TEIXEIRA, MANOEL DE ARAUJO; DE MELO, ITAMAR SOARES. ANTIFUNGAL COMPOUND PRODUCED BY THE CASSAVA ENDOPHYTE Bacillus pumilus MAIIIM4A. CIENTIA AGRICOL, v. 66, n. 5, p. 10-pg., 2009-01-01. (99/09177-1)

DE CARVALHO COSTA, FRANCISCO EDUARDO; DE MELO, ITAMAR SOARES. Endophytic and rhizospheric bacteria from Opuntia ficus-indica mill and their ability to promote plant growth in cowpea, Vigna unguiculata (L.) Walp. African Journal of Microbiology Research, v. 6, n. 6, p. 1345-1353, FEB 16 2012. (99/09177-1)

SIMIONATO, ANA VALERIA C.; SILVA-STENICO, MARIA ESTELA; TSAI, SIU MUI; CARRILHO, EMANUEL. Evidences of siderophores synthesis by Grapevine Xylella fastidiosa, causal agent of pierce's disease, through instrumental approaches. Journal of the Brazilian Chemical Society, v. 21, n. 4, p. 635-641, 2010. (08/57805-2, 99/09177-1)

FLÁVIA MANDOLESI PEREIRA DE MELO; MARLI FÁTIMA FIORE; LUIZ ALBERTO BERALDO DE MORAES; MARIA ESTELA SILVA-STENICO; SHIRLEI SCRAMIN; MANOEL DE ARAÚJO TEIXEIRA; ITAMAR SOARES DE MELO. Composto antifúngico produzido pelo endófito de mandioca Bacillus pumilus MAIIIM4a. Scientia Agricola, v. 66, n. 5, p. 583-592, 2009-10-00. (99/09177-1)