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In vitro evaluation of lipogenics and anti-adipogenics substances using three-dimensional model of adipocytes


The hyperplasia and hypertrophy of the adipocytes, generates a lot of dissatisfaction, especially in women, so research that reorganize the adipose tissue are frequent and necessary. Currently there is no in vitro model to test formulations for the treatment of cellulite and adipocyte dysfunctions, described in literature, even with the implementation of international and national laws and decrees urging the discontinuance of the use of animals in tests focused on ingredients and/or cosmetic products. Derived from plants and synthetic substances are widely used in cosmetic formulations due to multifunctional features that elevate the action potential. However, for the public use a screening must be conducted with regard to safety and efficacy. The goals of this proposal include the safety evaluation tests, based on the tests recommended by the OECD, such as cytotoxicity (OECD/GD 129), Phototoxicity (OECD 432) and the micronucleus in vitro (OECD 432). The efficacy test will be performed in three-dimensional levitation cell culture system based on magnetic particles which is the model ex vivo that it seems closer to the morphology and tissue interactions whereby in vivo. Thus, it will be possible to come up with an in vitro model to provide proof of effect claimed in cosmetics actives with the aim of replacement animal uses in research and development of cosmetic products. (AU)

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