Effect of Zinc Supplementation (Organic and Inorganic) on Proteomic and Metalomic Royal Jelly Profile, Hypopharyngeal Gland Morphology and Transcriptome of Apis mellifera Honey Bees

• Grant number: 18/00511-9
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2018
• End date: June 30, 2020
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Animal Production
• Principal Investigator: Ricardo de Oliveira Orsi
• Grantee: Ricardo de Oliveira Orsi
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Apis mellifera larval nutrition plays a fundamental role, directly influence its development as promoting a change in hormonal profile and integrity of queen's ovary. Minerals, such as zinc (Zn) shows, fundamental importance in structural and functional components of animals. However, the source of this mineral offered for animal nutrition may interfere with its bioavailability, promoting different effects on the animal physiology. Thus, in this project, we are proposing the following actions: 1) evaluate two sources of Zinc (organic and inorganic), in different concentrations, offered to A. mellifera bees; 2) stimulate royal jelly production; 3) evaluate proteomic and metalomic profile of royal jelly and correlate metalloproteins expression with food sources supplementation; 4) evaluate a possible interference of mineral source in six days old worker bees hypopharyngeal gland morphology 5) identify and analyze genes expression profile direct influenced by zinc through worker bees transcriptome analysis. For this, will be used 28 A. mellifera colonies, divided in 7 treatments: 1: Control - without supplementation; 2: Supplementation with 25 mg L-1 of inorganic zinc; 3: Supplementation with 25 mg L-1 of organic zinc; 4: Supplementation with 50 mg L-1 of inorganic zinc; 5: Supplementation with 50 mg L-1 of organic zinc; 6: Supplementation with 75 mg L-1 of inorganic zinc and; 7: Supplementation with 75 mg L-1 of organic zinc. Organic and inorganic zinc will be diluted in sugar syrup. The royal jelly production will be carried out by larvae transfer method, harvested 48 hours after production induction and will be storage in freezer (-20 ° C) until the moment of the proteomic analyzes. For hypopharyngeal glands development evaluation and transcriptome analysis will be collected 10 worker bees with six days old from each treatment. With this research project is expected to develop important information for honey bees nutrition, since it is an area with little scientific knowledge, which will certainly contributes to the advancement of the area, better nutrition and development of the colonies. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE BARROS, DANIEL C. B.; CAMILLI, MARCELO P.; MARTINELI, GABRIEL M.; LONGUINI, AIME A.; KADRI, SAMIR M.; JUSTULIN, LUIS A.; ORSI, RICARDO O.. Effect of organic and inorganic zinc supplementation on the development of mandibular glands in Apis mellifera. BULLETIN OF INSECTOLOGY, v. 74, n. 2, p. 209-212, DEC 2021. (18/00511-9)

LONGUINI, AIME DE ALMEIDA; MARTINELI, GABRIEL MORENO; CAMILLI, MARCELO POLIZEL; BRAMBILA DE BARROS, DANIEL CAVALCANTE; SOUZA VIEIRA, JOSE CAVALCANTE; PADILHA, PEDRO DE MAGALHAES; ORSI, RICARDO DE OLIVEIRA. Supplementation with an Inorganic Zinc Source in the Metalloproteomic Profile of Royal Jelly in Apis mellifera L.. BIOLOGICAL TRACE ELEMENT RESEARCH, JAN 2021. (18/00511-9)

CAMILLI, MARCELO P.; DE BARROS, DANIEL C. B.; MARTINELI, GABRIEL M.; LONGUINI, AIME A.; KADRI, SAMIR MOURA; VIEIRA, JOSE CAVALCANTE S.; DE MAGALHAES PADILHA, PEDRO; ORSI, RICARDO DE OLIVEIRA. Organic zinc supplementation modifies the metalloproteome of royal jelly produced by Apis mellifera. JOURNAL OF APICULTURAL RESEARCH, MAR 2021. (18/00511-9)

LONGUINI, AIME DE ALMEIDA; MARTINELI, GABRIEL MORENO; CAMILLI, MARCELO POLIZEL; BRAMBILA DE BARROS, DANIEL CAVALCANTE; SOUZA VIEIRA, JOSE CAVALCANTE; PADILHA, PEDRO DE MAGALHAES; ORSI, RICARDO DE OLIVEIRA. Supplementation with an Inorganic Zinc Source in the Metalloproteomic Profile of Royal Jelly in Apis mellifera L.. BIOLOGICAL TRACE ELEMENT RESEARCH, v. 199, n. 11, p. 4308-4318, NOV 2021. (18/00511-9)

CAMILLI, MARCELO POLIZEL; KADRI, SAMIR MOURA; NIZ ALVAREZ, MARCUS VINICIUS; MARTINS RIBOLLA, PAULO EDUARDO; ORSI, RICARDO OLIVEIRA. Zinc supplementation modifies brain tissue transcriptome of Apis mellifera honeybees. BMC Genomics, v. 23, n. 1, p. 13-pg., 2022-04-08. (18/00511-9)

CAMILLI, MARCELO P.; DE BARROS, DANIEL C. B.; MARTINELI, GABRIEL M.; LONGUINI, AIME A.; KADRI, SAMIR MOURA; VIEIRA, JOSE CAVALCANTE S.; DE MAGALHAES PADILHA, PEDRO; ORSI, RICARDO DE OLIVEIRA. Organic zinc supplementation modifies the metalloproteome of royal jelly produced by Apis mellifera. JOURNAL OF APICULTURAL RESEARCH, v. 62, n. 3, p. 8-pg., 2021-03-11. (18/00511-9)