Advanced search
Start date
Betweenand

EMU concedido no processo 2011/51295-5: image system

Grant number: 18/07177-7
Support type:Multi-user Equipment Program
Duration: June 01, 2018 - May 31, 2025
Field of knowledge:Biological Sciences - Parasitology
Principal Investigator:Célia Regina da Silva Garcia
Grantee:Célia Regina da Silva Garcia
Home Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated research grant:11/51295-5 - Functional genomics in Plasmodium, AP.TEM
As informações de acesso ao Equipamento Multiusuário são de responsabilidade do Pesquisador responsável
EMU web page:http://www.ib.usp.br/pesquisa/caimi-equipamentos
Tipo de equipamento:Processos Biológicos - Caracterização - Leitores de fluorescência (imageamento, placas)
Processos Biológicos - Caracterização - Leitores de placas
Fabricante: Nikon
Modelo: Ti2-E

Abstract

This equipment will allow us to use small amounts of parasites to perform real-time studies with parasites that express GFP to decipher the molecular mechanisms of calcium signaling. These studies of cellular physiology are fundamental in our research and will also be used for screening of compounds. Plasmodium falciparum has a life cycle of 48 hours. This new system will allow us to follow this temporal process, being fundamental for studies in the different stages. Other imaging studies will be carried out, as currently we are expressing in P. falciparum a genetically encoded fluorescent marker for mitochondria. This new parasites will allow us to follow the rearrangement of this organelle during the progression of the parasite cycle. In any case, the imaging system will be available to the USP community and external users. The acquisition of this equipment will enable the training of students and Pos docs in this important area and expand collaboration with brazilian and international research groups. (AU)