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Multi-user equipment approved in grant 15/19316-3: flow cytometer

Grant number: 18/12589-2
Support type:Multi-user Equipment Program
Duration: August 01, 2018 - July 31, 2025
Field of knowledge:Biological Sciences - Parasitology - Protozoology of Parasites
Principal Investigator:Mauro Ferreira de Azevedo
Grantee:Mauro Ferreira de Azevedo
Home Institution: Instituto de Saúde e Sociedade (ISS). Universidade Federal de São Paulo (UNIFESP). Campus Baixada Santista. Santos , SP, Brazil
Associated research grant:15/19316-3 - Regulation of P. falciparum egress from the host cell: identification of new targets, AP.JP
As informações de acesso ao Equipamento Multiusuário são de responsabilidade do Pesquisador responsável
EMU web page:https://www.unifesp.br/campus/san7/pesquisa/equipamentos-
Tipo de equipamento:Processos Biológicos - Caracterização - Contagem células (inclui citômetros)
Fabricante: Merck
Modelo: Guava easyCyte 5 System

Abstract

Despite successful efforts for its control, malaria is still a major public health problem in Brazil and in the World. The genome sequencing of several Plasmodium species as well as high through put transcriptomic and proteomic studies have allowed a better understanding about the parasite biology and its interaction with the host. In addition, thousands of compounds that inhibit parasite development and/or proliferation during in vitro cell culture have been identified, although the targets of most of them remain unknown. In a significantly lower scale, reverse genetics has allowed determining which genes encode for essential proteins for certain parasite stages and eventually to elucidate their function. Transgenic parasite lines have also been applied in the identification of compounds with anti-parasite effect and eventually in getting clues about their targets. In this project, new conditions for conditional gene expression will be evaluated in order to provide lower leakage and thus better regulation of the genes of interest. Parasite transgenic lines that are conditional knockout for protein kinases G or calcium-dependent protein kinase 5, which also express the super bright reporter Nano-Luciferase in the parasitophorous vacuole or in the red blood cell cytosol, will be applied to investigate the role of these kinases in regulating egress and to identify other molecules required for the process. The targets of potential new anti-malarial compounds will be investigated regarding their inhibitory effect on aspects of parasite biology such as protein secretion and export, invasion and egress from the host cell and the function of the new permeation pathway. (AU)