Biomolecules from plant species of remnant areas of the Atlantic Forest and Cerrado to treat neglected tropical diseases - chemical and pharmacological aspects

• Grant number: 18/07885-1
• Support Opportunities: BIOTA-FAPESP Program - Regular Research Grants
• Start date: August 01, 2018
• End date: July 31, 2020
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: João Henrique Ghilardi Lago
• Grantee: João Henrique Ghilardi Lago
• Host Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil
• Associated researchers: André Gustavo Tempone Cardoso ; Joao Luiz Baldim Zanin ; Luiz Felipe Domingues Passero ; Patricia Sartorelli
• Associated research grant(s): 19/13906-4 - Discovering new drug leads against Chagas Disease Based on Brazilian bioactive plant molecules, AP.R SPRINT

Abstract

In the present project will be selected plant species from Sao Paulo State biomes (Atlantic Forest and "Cerrado") aiming the structural characterization of natural products/semisynthetic derivatives with antiparasitic activity as well as evaluation of pre-clinical and toxicological potential, in continuation to the study begun in 2015 with FAPESP funding (project 2015/11936-2). Thus, different species belonging to Lauraceae, Piperaceae and Asteraceae will be collected and their extracts prepared, and those who had proven antiparasitic action (antileishmanial and antitrypanosomal) will be subjected to molecular dereplication procedures (HPLC/MS and NMR) followed to a bioassay-guided fractionation processes. Using this approach, the active metabolites will be purified and their structures will be determined by spectroscopic/spectrometric techniques. After this step, the crude extracts will be standardized by HPLC/DAD and HPLC/MS allowing the identification of the bioactive compound in the vegetable matrix (biomarker). After the recognition of the active substances, they will be submitted to SAR tests through different techniques aiming at the improvement of biological activities by molecular modifications guided by multivariate statistics and prediction models created according to each class of compounds obtained. Additionally, it is intended, in this proposal, to carry out specific tests to define the mode of action of substances with proven antiprotozoal activity in relation to the parasite, especially membrane pore evaluation and ultrastructural studies. Following the standardization biological assays will be carried out. Thus, this project intends to add new compounds and / or extracts with proven herbal pharmacological armamentarium to restricted activity, providing tools for the development of new herbal drugs. (AU)

Scientific publications

(50)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SANTOS, BEATRIZ MENDES; BEZERRA-SOUZA, ADRIANA; ARAGAKI, SONIA; RODRIGUES, ELIANA; UMEHARA, ERIC; GHILARDI LAGO, JOAO HENRIQUE; LAURENTI, MARCIA DALASTRA; RIBEIRO, SUSAN PEREIRA; DOMINGUES PASSERO, LUIZ FELIPE. Ethnopharmacology Study of Plants from Atlantic Forest with Leishmanicidal Activity. Evidence-based Complementary and Alternative Medicine, 2019. (18/07885-1)

GRECCO, SIMONE S.; COSTA-SILVA, THAIS A.; SOUSA, FERNANDA S.; CARGNELUTTI, STEFANO B.; UMEHARA, ERIC; MENDONCA, POLIANA S.; TEMPONE, ANDRE G.; LAGO, JOAO HENRIQUE G.. Neolignans isolated from twigs of Nectandra leucantha Ness & Mart (Lauraceae) displayed in vitro antileishmanial activity. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 24, SEP 27 2018. (18/07885-1, 15/23403-9)

TEMPONE, ANDRE G.; PIEPER, PAULINE; BORBOREMA, SAMANTA E. T.; THEVENARD, FERNANDA; LAGO, JOAO HENRIQUE G.; CROFT, SIMON L.; ANDERSON, EDWARD A.. Marine alkaloids as bioactive agents against protozoal neglected tropical diseases and malaria. NATURAL PRODUCT REPORTS, APR 2021. (19/10434-4, 18/10279-6, 18/07885-1)

BRITO, JULIANA R.; PASSERO, LUIZ FELIPE D.; BEZERRA-SOUZA, ADRIANA; LAURENTI, MARCIA D.; ROMOFF, PAULETE; BARBOSA, HENRIQUE; FERREIRA, EDGARD A.; LAGO, JOAO HENRIQUE G.. Antileishmanial activity and ultrastructural changes of related tetrahydrofuran dineolignans isolated from Saururus cernuus L. (Saururaceae). Journal of Pharmacy and Pharmacology, v. 71, n. 12, p. 1871-1878, DEC 2019. (18/07885-1, 16/00468-0)

MORAIS, THIAGO R.; ALVES CONSERVA, GEANNE A.; VARELA, MARINA T.; COSTA-SILVA, THAIS A.; THEVENARD, FERNANDA; PONCI, VITOR; FORTUNA, ANA; FALCAO, AMILCAR C.; TEMPONE, ANDRE G.; FERNANDES, JOAO PAULO S.; et al. Improving the drug-likeness of inspiring natural products - evaluation of the antiparasitic activity against Trypanosoma cruzi through semi-synthetic and simplified analogues of licarin A. SCIENTIFIC REPORTS, v. 10, n. 1, MAR 25 2020. (16/20633-6, 16/25028-3, 18/10279-6, 18/07885-1)

SILVA, ARMENIO A. DE CARVALHO A. DA; SOUSA, ELCILENE A. DE; VERAS, MARCIA D. ALVES; ARAUJO, BRUNO Q.; THEVENARD, FERNANDA; LAGO, JOAO HENRIQUE G.; COSTA-SILVA, THAIS A.; TEMPONE, ANDRE G.; CHAVES, MARIANA H.. In vitro anti-Trypanosoma cruzi evaluation of sesquiterpenes from the branches of Oxandra sessiliflora. PHYTOCHEMISTRY LETTERS, v. 37, p. 59-62, JUN 2020. (18/10279-6, 18/07885-1)

BARBOSA, HENRIQUE; DA SILVA, RAFAEL LEONARDO C. G.; COSTA-SILVA, THAIS A.; TEMPONE, ANDRE G.; ANTAR, GUILHERME M.; LAGO, JOAO HENRIQUE G.; CASELI, LUCIANO. Interaction of dicentrinone, an antitrypanosomal aporphine alkaloid isolated from Ocotea puberula (Lauraceae), in cell membrane models at the air-water interface. BIOORGANIC CHEMISTRY, v. 101, AUG 2020. (18/00291-9, 18/10279-6, 19/03239-0, 18/07885-1)

SANTANA, FERNANDA PAULA R.; THEVENARD, FERNANDA; GOMES, KAIO S.; TAGUCHI, LAURA; CAMARA, NIELS OLSEN S.; STILHANO, ROBERTA S.; URESHINO, RODRIGO P.; PRADO, CARLA MAXIMO; LAGO, JOAO HENRIQUE GHILARDI. New perspectives on natural flavonoids on COVID-19-induced lung injuries. Phytotherapy Research, v. 35, n. 9, p. 4988-5006, SEP 2021. (20/01221-4, 18/07885-1, 18/06088-0)

MENGARDA, ANA C.; SILVA, MARCOS P.; CIRINO, MARIA E.; MORAIS, THIAGO R.; CONSERVA, GEANNE A. A.; LAGO, JOAO HENRIQUE G.; DE MORAES, JOSUE. Licarin A, a neolignan isolated from Nectandra oppositifolia Nees & Mart. (Lauraceae), exhibited moderate preclinical efficacy against Schistosoma mansoni infection. Phytotherapy Research, v. 35, n. 9, p. 5154-5162, SEP 2021. (16/22488-3, 18/07885-1, 19/25289-0, 19/25905-2)

DA COSTA-SILVA, THAIS A.; ALVES CONSERVA, GEANNE A.; GALISTEO, JR., ANDRES J.; TEMPONE, ANDRE G.; LAGO, JOAO HENRIQUE G.. Antileishmanial activity and immunomodulatory effect of secosubamolide, a butanolide isolated from Nectandra oppositifolia (Lauraceae). Journal of Venomous Animals and Toxins including Tropical Diseases, v. 25, AUG 19 2019. (16/20633-6, 18/10279-6, 18/07885-1)

PONCI, VITOR; SILVA, RAFAEL C.; SANTANA, FERNANDA PAULA R.; GRECCO, SIMONE S.; FORTUNATO, CELIA REGINA M.; OLIVEIRA, MARIA A.; TAVARES-DE-LIMA, WOTHAN; OLIVO, CLARICE R.; CALVO TIBERIO, IOLANDA DE FATIMA L.; GOMES, KAIO S.; et al. Biseugenol Exhibited Anti-Inflammatory and Anti-Asthmatic Effects in an Asthma Mouse Model of Mixed-Granulocytic Asthma. Molecules, v. 25, n. 22, NOV 2020. (18/06088-0, 18/07885-1)

FERREIRA, DAIANE D.; SOUSA, FERNANDA S.; COSTA-SILVA, THAIS A.; REIMAO, JULIANA Q.; TORRECILHAS, ANA C.; JOHNS, DEIDRE M.; SEAR, CLAIRE E.; HONORIO, KATHIA M.; LAGO, JOAO HENRIQUE G.; ANDERSON, EDWARD A.; et al. Dehydrodieugenol B derivatives as antiparasitic agents: Synthesis and biological activity against Trypanosoma cruzi. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, v. 176, p. 162-174, AUG 15 2019. (15/50075-2, 18/10279-6, 18/07885-1, 16/24524-7)

OLIVEIRA, EMERSON A.; MARTINS, EUDER G. A.; SOARES, MARISI G.; CHAGAS-PAULA, DANIELA A.; PASSERO, LUIZ F. D.; SARTORELLI, PATRICIA; BALDIM, JOAO L.; LAGO, JOAO HENRIQUE G.. A Comparative Study on Chemical Composition, Antileishmanial and Cytotoxic Activities of the Essential Oils from Leaves of Guarea macrophylla (Meliaceae) from Two Different Regions of São Paulo State, Brazil, Using Multivariate Statistical Analysis. Journal of the Brazilian Chemical Society, v. 30, n. 7, p. 1395-1405, JUL 2019. (16/00468-0, 18/07885-1, 16/19269-8)

AMARAL, MAIARA; DE SOUSA, FERNANDA S.; SILVA, THAIS A. COSTA; JUNIOR, ANDRES JIMENEZ G.; TANIWAKI, NOEMI N.; JOHNS, DEID RE M.; LAGO, JOAO HENRIQUE G.; ANDERSON, EDWARD A.; TEMPONE, ANDRE G.. A semi-synthetic neolignan derivative from dihydrodieugenol B selectively affects the bioenergetic system of Leishmania infantum and inhibits cell division. SCIENTIFIC REPORTS, v. 9, APR 16 2019. (15/50075-2, 18/10279-6, 18/07885-1)

VARELA, MARINA T.; COSTA-SILVA, THAIS A.; LAGO, JOAO HENRIQUE G.; TEMPONE, ANDRE G.; FERNANDES, JOAO PAULO S.. Evaluation of the antitrypanosoma activity and SAR study of novel LINS03 derivatives. BIOORGANIC CHEMISTRY, v. 89, AUG 2019. (18/03918-2, 16/25028-3, 18/07885-1, 15/23403-9)

ALVES CONSERVA, GEANNE A.; DA COSTA-SILVA, THAIS A.; AMARAL, MAIARA; ANTAR, GUILHERME M.; NEVES, BRUNO J.; ANDRADE, CAROLINA H.; TEMPONE, ANDRE G.; LAGO, JOAO HENRIQUE G.. Butenolides from Nectandra oppositifolia (Lauraceae) displayed anti-Trypanosoma cruzi activity via deregulation of mitochondria. Phytomedicine, v. 54, p. 302-307, FEB 15 2019. (16/20633-6, 15/23403-9, 18/07885-1)

BRITO, JULIANA R.; DA COSTA-SILVA, THAIS A.; TEMPONE, ANDRE G.; FERREIRA, EDGARD A.; LAGO, JOAO HENRIQUE G.. Dibenzylbutane neolignans from Saururus cernuus L. (Saururaceae) displayed anti-Trypanosoma cruzi activity via alterations in the mitochondrial membrane potential. Fitoterapia, v. 137, SEP 2019. (18/10279-6, 18/07885-1)

DE SOUSA, FERNANDA S.; NUNES, EMILENE A.; GOMES, KAIO S.; CERCHIARO, GISELLE; LAGO, JOAO HENRIQUE G.. Genotoxic and cytotoxic effects of neolignans isolated from Nectandra leucantha (Lauraceae). TOXICOLOGY IN VITRO, v. 55, p. 116-123, MAR 2019. (15/11936-2, 14/24475-0, 15/04143-6, 18/07885-1)

BITTENCOURT-MERNAK, I, MARCIA; PINHEIRO, NATHALIA M.; DA SILVA, RAFAEL C.; PONCI, VITOR; BANZATO, ROSANA; PINHEIRO, ARUANA J. M. C. R.; OLIVO, CLARICE R.; TIBERIO, IOLANDA F. L. C.; LIMA NETO, LIDIO G.; SANTANA, FERNANDA P. R.; et al. Effects of Eugenol and Dehydrodieugenol B from Nectandra leucantha against Lipopolysaccharide (LPS)-Induced Experimental Acute Lung Inflammation. Journal of Natural Products, v. 84, n. 8, p. 2282-2294, AUG 27 2021. (18/07885-1, 18/06088-0)

JESUS, JESSICA ADRIANA; FRAGOSO DA SILVA, THAYS NICOLLI; YAMAMOTO, EDUARDO SEIJI; G. LAGO, JOAO HENRIQUE; DALASTRA LAURENTI, MARCIA; PASSERO, LUIZ FELIPE DOMINGUES. Ursolic Acid Potentializes Conventional Therapy in Experimental Leishmaniasis. PATHOGENS, v. 9, n. 10, OCT 2020. (18/24077-6, 18/07885-1, 16/00468-0, 16/10324-6)

SESSA, DEBORAH P.; MENGARDA, ANA C.; SIMPLICIO, PAULA E.; ANTAR, GUILHERME M.; LAGO, JOAO HENRIQUE G.; DE MORAES, JOSUE. 15 beta-Senecioyl-oxy-ent-kaur-16-en-19-oic Acid, a Diterpene Isolated from Baccharis lateralis, as Promising Oral Compound for the Treatment of Schistosomiasis. Journal of Natural Products, v. 83, n. 12, p. 3744-3750, DEC 24 2020. (16/22488-3, 18/07885-1)

IVANILDO A. BRITO; EMERSON A. OLIVEIRA; MARIANA H. CHAVES; FERNANDA THEVENARD; ANDRÉ F. RODRIGUES-OLIVEIRA; GUSTAVO BARBOSA-REIS; PATRICIA SARTORELLI; DIOGO OLIVEIRA-SILVA; ANDRE G. TEMPONE; THAIS A. COSTA-SILVA; et al. Antileishmanial Acetylene Fatty Acid and Acetogenins from Seeds of Porcelia macrocarpa. Journal of the Brazilian Chemical Society, v. 32, n. 2, p. 447-453, 2021-02-01. (18/07885-1, 18/10279-6)

GRECCO, SIMONE S.; COSTA-SILVA, THAIS A.; SOUSA, FERNANDA S.; CARGNELUTTI, STEFANO B.; UMEHARA, ERIC; MENDONCA, POLIANA S.; TEMPONE, ANDRE G.; LAGO, JOAO HENRIQUE G.. Neolignans isolated from twigs of Nectandra leucantha Ness & Mart (Lauraceae) displayed in vitro antileishmanial activity. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 24, p. 7-pg., 2018-09-27. (15/23403-9, 18/07885-1)

SANTOS, BEATRIZ MENDES; BEZERRA-SOUZA, ADRIANA; ARAGAKI, SONIA; RODRIGUES, ELIANA; UMEHARA, ERIC; GHILARDI LAGO, JOAO HENRIQUE; LAURENTI, MARCIA DALASTRA; RIBEIRO, SUSAN PEREIRA; DOMINGUES PASSERO, LUIZ FELIPE. Ethnopharmacology Study of Plants from Atlantic Forest with Leishmanicidal Activity. Evidence-based Complementary and Alternative Medicine, v. 2019, p. 8-pg., 2019-01-01. (18/07885-1)

MENGARDA, ANA C.; SILVA, MARCOS P.; CIRINO, MARIA E.; MORAIS, THIAGO R.; CONSERVA, GEANNE A. A.; LAGO, JOAO HENRIQUE G.; DE MORAES, JOSUE. Licarin A, a neolignan isolated from Nectandra oppositifolia Nees & Mart. (Lauraceae), exhibited moderate preclinical efficacy against Schistosoma mansoni infection. Phytotherapy Research, JUN 2021. (16/22488-3, 19/25289-0, 19/25905-2, 18/07885-1)

ARAUJO, SHEILA C.; SOUSA, FERNANDA S.; COSTA-SILVA, THAIS A.; TEMPONE, ANDRE G.; LAGO, JOAO HENRIQUE G.; HONORIO, KATHIA M.. Discovery of New Hits as Antitrypanosomal Agents by In Silico and In Vitro Assays Using Neolignan-Inspired Natural Products from Nectandra leucantha. Molecules, v. 26, n. 14, JUL 2021. (18/07885-1, 21/04464-8, 16/24524-7, 21/02789-7)

REIS, ISABELLA MARY A.; UMEHARA, ERIC; CONCEICAO, RODRIGO S.; DE M. OLIVEIRA, LARISSA; DOS S. JUNIOR, MANOELITO COELHO; COSTA-SILVA, THAIS A.; AMARAL, MAIARA; TEMPONE, ANDRE G.; BRANCO, ALEXSANDRO; LAGO, JOAO HENRIQUE G.. gamma-Lactones from Persea americana and Persea fulva - in Vitro and in Silico Evaluation of Trypanosoma cruzi Activity. CHEMISTRY & BIODIVERSITY, v. 18, n. 9, JUL 2021. (18/07885-1, 21/02789-7)

PAROLIN, GIOVANA A.; GONCALVES, GIULIA E. G.; COSTA-SILVA, THAIS A.; TEMPONE, ANDRE G.; CASELI, LUCIANO; LAGO, JOAO HENRIQUE G.; PERES, LAURA O.. Evaluation of the effects in cellular membrane models of antitrypanosomal poly-thymolformaldehyde (PTF) using Langmuir monolayers. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, v. 1863, n. 2, FEB 1 2021. (18/22214-6, 14/50869-6, 18/07885-1, 18/18180-9, 18/10279-6, 19/03239-0)

GALHARDO, THALITA S.; UENO, ANDERSON K.; COSTA-SILVA, THAIS A.; TEMPONE, ANDRE G.; CARVALHO, WAGNER A.; FISCHMEISTER, CEDRIC; BRUNEAU, CHRISTIAN; MANDELLI, DALMO; LAGO, JOAO HENRIQUE G.. New derivatives from dehydrodieugenol B and its methyl ether displayed high anti-Trypanosoma cruzi activity and cause depolarization of the plasma membrane and collapse the mitochondrial membrane potential. Chemico-Biological Interactions, v. 366, p. 6-pg., 2022-09-07. (18/01258-5, 18/10279-6, 21/02789-7, 21/04464-8, 17/17044-1, 16/05006-5, 18/07885-1)

OLIVEIRA, EMERSON A.; BRITO, IVANILDO A.; LIMA, MARTA L.; ROMANELLI, MAIARA; MOREIRA-FILHO, JOSE T.; NEVES, BRUNO J.; ANDRADE, CAROLINA H.; SARTORELLI, PATRICIA; TEMPONE, ANDRE G.; COSTA-SILVA, THAIS A.; et al. Antitrypanosomal Activity of Acetogenins Isolated from the Seeds of Porcelia macrocarpa Is Associated with Alterations in Both Plasma Membrane Electric Potential and Mitochondrial Membrane Potential. Journal of Natural Products, v. 82, n. 5, p. 1177-1182, MAY 2019. (18/10279-6, 18/07885-1)

MORAIS, THIAGO R.; COSTA-SILVA, THAIS A.; FERREIRA, DAIANE D.; NOVAIS, BIANCA J.; TORRECILHAS, ANA CLAUDIA T.; TEMPONE, ANDRE G.; LAGO, JOAO HENRIQUE G.. Antitrypanosomal activity and effect in plasma membrane permeability of (-)-bornyl p-coumarate isolated from Piper cernuum (Piperaceae). BIOORGANIC CHEMISTRY, v. 89, AUG 2019. (18/10279-6, 18/07885-1)

YAMAMOTO, EDUARDO SEIJI; DE JESUS, JESSICA ADRIANA; BEZERRA-SOUZA, ADRIANA; BRITO, JULIANA R.; LAGO, JOAO HENRIQUE G.; LAURENTI, MARCIA DALASTRA; PASSERO, LUIZ FELIPE DOMINGUES. Tolnaftate inhibits ergosterol production and impacts cell viability of Leishmania sp.. BIOORGANIC CHEMISTRY, v. 102, SEP 2020. (15/17623-6, 18/07885-1, 16/00468-0)

GOMES, KAIO S.; DA COSTA-SILVA, THAIS A.; OLIVEIRA, IGOR H.; AGUILAR, ANDREA M.; OLIVEIRA-SILVA, DIOGO; UEMI, MIRIAM; SILVA, WENDER A.; MELO, LENNINE R.; ANDRADE, CARLOS KLEBER Z.; TEMPONE, ANDRE G.; et al. Structure-activity relationship study of antitrypanosomal chalcone derivatives using multivariate analysis. Bioorganic & Medicinal Chemistry Letters, v. 29, n. 12, p. 1459-1462, JUN 15 2019. (18/10279-6, 18/07885-1)

MENGARDA, ANA C.; MENDONCA, POLIANA S.; MORAIS, CRISTIANE S.; COGO, RAMON M.; MAZLOUM, SUSANA F.; SALVADORI, MARIA C.; TEIXEIRA, FERNANDA S.; MORAIS, THIAGO R.; ANTAR, GUILHERME M.; LAGO, JOAO HENRIQUE G.; et al. Antiparasitic activity of piplartine (piperlongumine) in a mouse model of schistosomiasis. Acta Tropica, v. 205, MAY 2020. (18/07885-1, 16/22488-3)

LONDERO, VINICIUS S.; COSTA-SILVA, THAIS A.; TEMPONE, ANDRE G.; NAMIYAMA, GISLENE M.; THEVENARD, FERNANDA; ANTAR, GUILHERME M.; BAITELLO, JOAO B.; LAGO, JOAO HENRIQUE G.. Anti-Trypanosoma cruzi activity of costic acid isolated from Nectandra barbellata (Lauraceae) is associated with alterations in plasma membrane electric and mitochondrial membrane potentials. BIOORGANIC CHEMISTRY, v. 95, JAN 2020. (18/10279-6, 17/17044-1, 18/07885-1)

PAROLIN, GIOVANA A.; PASSERO, LUIZ FELIPE D.; LAGO, JOAO HENRIQUE G.; PERES, LAURA OLIVEIRA. Antileishmanial activity evaluation of poly(thymolformaldehyde)-A biobased material from thymol. INDUSTRIAL CROPS AND PRODUCTS, v. 171, NOV 1 2021. (18/07885-1, 18/24077-6, 18/18180-9, 14/50869-6)

BARBOSA, HENRIQUE; COSTA-SILVA, THAIS A.; ALVES CONSERVA, GEANNE A.; ARAUJO, ADELSON J.; LORDELLO, ANA LUISA L.; ANTAR, GUILHERME M.; AMARAL, MAIARA; SOARES, MARISI G.; TEMPONE, ANDRE G.; LAGO, JOAO HENRIQUE G.. Aporphine Alkaloids from Ocotea puberula with Anti-Trypanosoma Cruzi Potential - Activity of Dicentrine-beta-N-Oxide in the Plasma Membrane Electric Potentials. CHEMISTRY & BIODIVERSITY, v. 18, n. 4, MAR 2021. (18/10279-6, 18/07885-1)

GRECCO, SIMONE DOS SANTOS; LETSYO, EMMANUEL; TEMPONE, ANDRE GUSTAVO; GHILARDI LAGO, JOAO HENRIQUE; JERZ, GEROLD. Electrospray mass-spectrometry guided target isolation of neolignans from Nectandra leucantha (Lauraceae) by high performance- and spiral-coil countercurrent chromatography. Journal of Chromatography A, v. 1608, DEC 20 2019. (18/07885-1, 18/09083-0)

DE SOUSA, FERNANDA S.; BALDIM, JOAO L.; AZEVEDO, RICARDO A.; FIGUEIREDO, CARLOS R.; PIEPER, PAULINE; SEAR, CLAIRE E.; ANDERSON, EDWARD A.; LAGO, JOAO HENRIQUE G.. Structure-activity relationship study of cytotoxic neolignan derivatives using multivariate analysis and computation-aided drug design. Bioorganic & Medicinal Chemistry Letters, v. 30, n. 16, AUG 15 2020. (15/11936-2, 15/04143-6, 16/19269-8, 18/07885-1)

FERREIRA, BRUNO ANTONIO; NORTON FILHO, ANDERSON FERRAZ; DECONTE, SIMONE RAMOS; TOMIOSSO, TATIANA CARLA; THEVENARD, FERNANDA; ANDRADE, SILVIA PASSOS; GHILARDI LAGO, JOAO HENRIQUE; ARAUJO, FERNANDA DE ASSIS. Sesquiterpene Polygodial from Drimys brasiliensis (Winteraceae) Down-Regulates Implant-Induced Inflammation and Fibrogenesis in Mice. Journal of Natural Products, v. 83, n. 12, p. 3698-3705, DEC 24 2020. (18/07885-1)

GONCALVES, GIULIA ELISA G.; MORAIS, THIAGO R.; LAGO, JOAO HENRIQUE G.; CASELI, LUCIANO. Incorporation of polygodial in Langmuir films of selected lipids. Thin Solid Films, v. 669, p. 19-28, JAN 1 2019. (15/23446-0, 18/07885-1)

GUIMARAES GONCALVES, GIULIA ELISA; MORAIS, THIAGO RAHAL; GOMES, KAIO DE SOUZA; COSTA-SILVA, THAIS ALVES; TEMPONE, ANDRE GUSTAVO; GHILARDI LAGO, JOAO HENRIQUE; CASELI, LUCIANO. Antitrypanosomal activity of epi-polygodial from Drimys brasiliensis and its effects in cellular membrane models at the air-water interface. BIOORGANIC CHEMISTRY, v. 84, p. 186-191, MAR 2019. (15/23446-0, 13/14262-7, 18/07885-1, 15/23403-9)

UMEHARA, ERIC; SILVA, THAIS A. COSTA; MENDES, VIVIANE M.; GUADAGNIN, RAFAEL C.; SARTORELLI, PATRICIA; TEMPONE, ANDRE G.; LAGO, JOAO HENRIQUE G.. Differential lethal action of C17:2 and C17:0 anacardic acid derivatives in Trypanosoma cruzi - A mechanistic study. BIOORGANIC CHEMISTRY, v. 102, SEP 2020. (18/10279-6, 18/07885-1)

LONDERO, VINICIUS S.; COSTA-SILVA, THAIS A.; ANTAR, GUILHERME M.; BAITELLO, JOAO B.; DE OLIVEIRA, LARISSA V. F.; CAMILO, FERNANDA F.; BATISTA, ANDREA N. L.; BATISTA, JR., JOAO M.; TEMPONE, ANDRE G.; LAGO, JOAO HENRIQUE G.. Antitrypanosomal Lactones from Nectandra barbellata. Journal of Natural Products, v. 84, n. 5, p. 1489-1497, MAY 28 2021. (14/25222-9, 18/07885-1, 21/02789-7, 18/10279-6, 17/17044-1)

TOTINI, CARLOS H.; UMEHARA, ERIC; REIS, ISABELLA M. A.; LAGO, JOAO HENRIQUE G.; BRANCO, ALEXSANDRO. Chemistry and Bioactivity of the Genus Persea - A Review. CHEMISTRY & BIODIVERSITY, v. N/A, p. 18-pg., 2023-08-22. (21/02789-7, 18/07885-1)

DA SILVA, FLAVIA T.; GOMES, KAIO S.; MACHADO, FABRICIO C.; LOUREIRO, LETICIA L.; TRAVASSOS, LUIZ R.; MARTINS, TEREZA S.; LAGO, JOAO HENRIQUE G.; CAMILO, FERNANDA F.. Electrochemical Preparation and Evaluation of Cytotoxic Activity of Methoxyl-oxo-biseugenol, a New Oxidized Derivative of Biseugenol. Journal of the Brazilian Chemical Society, v. 32, n. 5, p. 1002-1008, MAY 2021. (18/20826-4, 18/07885-1)

TEMPONE, ANDRE G.; PIEPER, PAULINE; BORBOREMA, SAMANTA E. T.; THEVENARD, FERNANDA; LAGO, JOAO HENRIQUE G.; CROFT, SIMON L.; ANDERSON, EDWARD A.. Marine alkaloids as bioactive agents against protozoal neglected tropical diseases and malaria. NATURAL PRODUCT REPORTS, v. 38, n. 12, p. 2214-2235, DEC 15 2021. (18/10279-6, 19/10434-4, 18/07885-1)

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