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MicroRNA profile in the mesenteric adipose tissue of Crohn's disease patients

Grant number: 18/05584-4
Support Opportunities:Regular Research Grants
Duration: September 01, 2018 - November 30, 2020
Field of knowledge:Biological Sciences - Immunology - Immunogenetics
Principal Investigator:Raquel Franco Leal
Grantee:Raquel Franco Leal
Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Associated researchers:Adriana Souza Torsoni ; Licio Augusto Velloso ; Marciane Milanski Ferreira ; Maria de Lourdes Setsuko Ayrizono
Associated scholarship(s):20/03418-0 - MicroRNA profile in the mesenteric adipose tissue of Crohn's disease patients, BP.TT

Abstract

Crohn's disease (CD) is a chronic intestinal disorder with a multifactorial etiology, whose incidence has increased during the last three decades. Intestinal involvement is transmural, so there is a possibility of developing fistulas and strictures. The role of mesenteric adipose tissue (MAT) in the development of these complications, as well as in the disease's etiology is not well established. The characteristic macroscopic increase of intestinal MAT near the affected intestinal area, with circumferential involvement of the intestinal loop, was noticed since the first description of CD, but there are few studies that address this issue. In this study, we intend to evaluate MAT samples from 20 patients with ileocecal CD, and from 10 control subjects. Validation of the transcriptional study (RNA-sequencing) will be performed focusing on microRNA expression in a cohort independent of the one in which RNAseq was performed. To achieve this goal, this validation will be performed by RT-PCR. In addition, we will identify the targets of interest of these microRNAs through in silico analysis in a free microRNAs database, miRWalk 2.0. From this analysis, transcripts will be selected and validated by RT-PCR. We will also correlate the findings of microRNA expression with the epidemiological and clinical data of the patients. Thus, this study may identify tissue-specific differences that could be involved in the susceptibility to the disease, and may also identify potential biomarkers and targets of pharmacological agents, individualizing therapies. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
KARINE, STEIGLEDER; LIVIA, PASCOAL; LAIS, SIMINO; FRANCESCA, SILVA; NATALIA, SIQUEIRA; DE LOURDES, AYRIZONO MARIA; JOAO, FAGUNDES; ADRIANA, TORSONI; RAQUEL, LEAL. Corticosteroids, Aminosalicylates and Gastrointestinal Symptoms Are Associated With the Need of Hospitalization in Patients With Inflammatory Bowel Diseases and COVID-19. AMERICAN JOURNAL OF GASTROENTEROLOGY, v. 115, p. 2-pg., . (18/05584-4)

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Filed patent(s) as a result of this research project

BIOMARCADOR PARA PROGNÓSTICO DA DOENÇA DE CROHN NO PÓSOPERATÓRIO - BIOPROG_CROHN BR512021001121-0 - Universidade Estadual de Campinas (UNICAMP) . Raquel Franco Leal ; Karine Mariane Steigleder ; Anibal Tavares De Azevedo ; Adriana Souza Torsoni ; Livia Aparecida D'avilla Bitencourt Pascoal Biazzo ; Laís Angélica De Paula Simino ; Marciane Milanski Ferreira ; Maria De Lourdes Setsuko Ayrizono ; João José Fagundes - May 2021, 26