Sustainable use of the Brazilian biodiversity: chemical and pharmacological prospection on higher plants

• Grant number: 02/05503-6
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
• Start date: August 01, 2003
• End date: December 31, 2008
• Field of knowledge: Physical Sciences and Mathematics - Chemistry
• Principal Investigator: Wagner Vilegas
• Grantee: Wagner Vilegas
• Host Institution: Instituto de Química (IQ). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Pesquisadores principais: Alba Regina Monteiro Souza Brito ; Clarice Queico Fujimura Leite ; Eliana Aparecida Varanda
• Associated research grant(s): 06/55994-7 - In vitro evaluation of byrsonima crassa to treat helicobacter pylori infeciton., AR.EXT
• Associated scholarship(s): 08/01682-0 - Sustentable use of the Brazilian biodiversity: Evaluation of chemical-pharmacological of the hight plants:Indigofera hirsuta (Fabaceae), BP.MS; 08/05921-9 - Sustainable Use of the Brazilian Biodiversity: Chemical and Pharmacological Prospection on Higher Plants: Chemical study of infuses from Byrsonima coccolobifolia and Byrsonima ligustrifolia (Malpighiaceae), BP.IC; 07/53362-6 - Mechanisms of action involved in antiulcerogenic activity of Anacardium humile St. Hil. (Anacardiaceae), BP.MS; + associated scholarships 07/51048-2 - Sustainable use of Brazilian biodiversity: chemical-pharmacological evaluation of higher plants Serjania grandiflora Cambess (Sapindaceae), BP.IC; 06/61772-7 - Sustainable use of Brazilian biodiversity: chemical pharmacological prospection in higher plants: development of chromatographic methodologies for establishment of qualitative and quantitative profiles, BP.PD; 06/51453-1 - Sustainable use of Brazilian biodiversity: chemical-pharmacological prospection in higher plants: methodology for the establishment of qualitative and quantative profiles for vegetal extracts, BP.DR; 06/57512-0 - Sustainable use of Brazilian biodiversity: chemical-pharmacological evaluation of higher plants: Guapira spp., BP.DR; 06/60139-9 - Sustainable use of Brazilian biodiversity: chemical pharmacological evaluation of higher plants: Indigofera suffruticosa (Fabaceae), BP.IC; 06/56469-3 - Sustainable use of Brazilian biodiversity: chemical pharmacological prospection in higher plants: Serjania (Sapindaceae) Will., BP.PD; 05/60835-2 - Sustainable use of Brazilian biodiversity: chemical-pharmacological evaluation of higher plants: Mouriri elliptica (Melastomataceae), BP.IC; 05/54984-5 - Morphoanatomy of the vegetative organs and chemical profile of species of Smilax L. (Smilacaceae) genus, BP.DR; 04/10736-5 - Sustainable use of Brazilian biodiversity: Chemical-pharmacological prospection in higher plants: Neea spp. (Nyctaginaceae), BP.MS; 04/12967-4 - Sustainable use of Brazilian biodiversity: chemical-pharmacological evaluation of higher plants: Guapira noxia, BP.MS; 04/13660-0 - Determination of the antioxidant activity involved in antiulcerogenic mechanisms of Anacardium humile St. Hill. (Anacardiaceae), BP.IC; 04/07898-3 - Determination of the antioxidant activity of plants of the Indigofera and Vernonia genus, BP.PD; 04/07255-5 - Sustainable use of Brazilian biodiversity: chemical pharmacological prospection in higher plants, BP.DR; 04/03101-3 - Sustainable use of Brazilian biodiversity: Chemical-pharmacological prospection in higher plants: Alchornea (Euphorbiaceae) and Indigofera (Fabaceae), BP.DD; 03/09748-6 - Chemical-pharmacological prospection in higher plants: activity of Mouriri ssp. on the gastrointestinal system, BP.DR; 04/03289-2 - Chemical-pharmacological prospection in higher plants: activity of Mangifera indica Linn. on the gastrointestinal system, BP.IC; 04/01436-8 - Study of the antimycobacterial activity of vegetable extracts, BP.IC; 04/01786-9 - Evaluation of the mutagenic activity of the plants Ananas ananassoides and Hancornia speciosa through trials of reverse genetic mutation with S. typhimurium, BP.IC; 04/01787-5 - Trials of reverse genetic mutation with Salmonella typhimurium for evaluation of mutagenic activity of the plants Davilla nitida e D. elliptica, BP.IC; 04/00943-3 - Medicinal vivarium project: ethnopharmacology as a tool for a program of environmental education in the town of Sumaré, SP, BP.IC; 03/10631-6 - Sustainable use of Brazilian biodiversity: Chemical-pharmacological prospection in higher plants: Davilla elliptica St. Hill, BP.IC; 03/07809-8 - Sustainable use of Brazilian biodiversity: chemical-pharmacological prospection in higher plants: chemical study of Qualea, BP.IC; 03/03110-0 - Antiulcerogenic activity of native plants of the cerrado in the State of São Paulo belonging to the Indigofera genus, BP.DD; 02/09493-5 - Sustainable use of Brazilian biodiversity: pharmacological chemical prospection in higher plants: Byrsonima (Malpighiaceae), BP.PD - associated scholarships

Abstract

The apparent incompatibility between chemical and pharmacological investigation of a plant species can be solved with the strong determination of dealing rationally with the problem. The research groups on Natural Products Chemistry from the IQ-Araraquara-Unesp, on Pharmacology, from IB-Unicamp and 1B-Botucatu-Unesp and on Biological Sciences, from FCF-Araraquara-Unesp have started a collaboration 2 years ago, and have already produced a significative amount of work, with relevant results on the investigation of plant species with anti-ulcer, anti-oxidant, analgesic, anti-inflammatory and antimicrobial activities. hese results arose from an approach that includes ethnobotanical and ethnopharmacological research, pharmacological, microbiological and mutagenicity assays with crude extracts or infusions, chemical screening to determine new chemical classes of compounds, isolation and structural determination of compounds and use the compounds or enriched fractions to determine the possible pharmacological mechanisms of the action involved with the detected properties. This project deals with the continuation of the integrated chemical and pharmacological investigation of plant extracts, this time investigating species that com poses the bioma Cerrado of the State São Paulo, comparatively to the Bioma Cerrado of the State of Tocantins, which it is also under our investigation. To perform the phytochemical step we intend to use the usual chromatographic techniques, mainly those for the analysis of polar substances (GPC, XAD2, DCCC, HSCC, HPLC, etc), and to determine the structure of the isolated compounds through spectrometric methods (NMR, IR, UV, and MS). To evaluate the biological activities, the possibility of toxicicity and genotoxicity effects of each species will be determined. Simultaneously, we will evaluate the activity of the extracts and pure substances (ar enriched fractions) against different experimental models of peptic ulcer disease which operate by distinct mechanism of ulcerogenesis in man... (AU)

Scientific publications

(14)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

HIGUCHI, C. T.; SANNOMIYA, M.; PAVAN, F. R.; LEITE, S. R. A.; SATO, D. N.; FRANZBLAU, S. G.; SACRAMENTO, L. V. S.; VILEGAS, W.; LEITE, C. Q. F.. Byrsonima fagifolia Niedenzu Apolar Compounds with Antitubercular Activity. Evidence-based Complementary and Alternative Medicine, 2011. (02/05503-6)

FERNANDO ROGÉRIO PAVAN; CLARICE QUEICO FUJIMURA LEITE; ROBERTA GOMES COELHO; ISABEL DUARTE COUTINHO; NELI KIKA HONDA; CLAUDIA ANDRÉA LIMA CARDOSO; WAGNER VILEGAS; SERGIO ROBERTO DE ANDRADE LEITE; DAISY NAKAMURA SATO. Evaluation of anti-Mycobacterium tuberculosis activity of Campomanesia adamantium (Myrtaceae). Química Nova, v. 32, n. 5, p. 1222-1226, 2009-00-00. (02/05503-6, 08/10390-2)

CARLI, CAMILA B. DE A.; DE MATOS, DJAMILE C.; LOPES, FLAVIA C. M.; MAIA, DANIELLE C. G.; DIAS, MARISTELA B.; SANNOMIYA, MIRIAM; RODRIGUES, CLENILSON M.; ANDREO, MARCIO A.; VILEGAS, WAGNER; COLOMBO, LUCAS L.; et al. Isolated Flavonoids against Mammary Tumour Cells LM2. ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES, v. 64, n. 1-2, p. 32-36, JAN-FEB 2009. (02/05503-6)

HIRUMA-LIMA, CLÉLIA AKIKO; CALVO, TAMARA ROBERTA; RODRIGUES, CLENILSON MARTINS; ANDRADE, FÁBIO DONIZETE PEZZUTO; VILEGAS, WAGNER; BRITO, ALBA REGINA MONTEIRO SOUZA. Antiulcerogenic activity of Alchornea castaneaefolia: Effects on somatostatin, gastrin and prostaglandin. Journal of Ethnopharmacology, v. 104, n. 1/2, p. 215-224, Mar. 2006. (02/05503-6)

RODRIGUES, CLENILSON M.; RINALDO, DANIEL; SANTOS, LOURDES C. DOS; MONTORO, PAOLA; PIACENTE, SONIA; PIZZA, COSIMO; HIRUMA-LIMA, CLÉLIA A.; BRITO, ALBA R. M. SOUZA; VILEGAS, WAGNER. Metabolic fingerprinting using direct flow injection electrospray ionization tandem mass spectrometry for the characterization of proanthocyanidins from the barks of Hancornia speciosa. Rapid Communications in Mass Spectrometry, v. 21, n. 12, p. 1907-1914, June 2007. (02/05503-6)

LOPES, FLAVIA C. M.; CALVO, TAMARA R.; COLOMBO, LUCAS L.; VILEGAS, WAGNER; CARLOS, IRACILDA Z.. Immunostimulatory and cytotoxic activities of Indigofera suffruticosa (Fabaceae). NATURAL PRODUCT RESEARCH, v. 25, n. 19, p. 1796-1806, 2011. (02/05503-6)

SANNOMIYA, MIRIAM; SANTOS, LOURDES CAMPANER DOS; CARBONE, VIRGINIA; NAPOLITANO, ASSUNTA; PIACENTE, SONIA; PIZZA, COSIMO; SOUZA-BRITO, ALBA R. M.; VILEGAS, WAGNER. Liquid chromatography/electrospray ionization tandem mass spectrometry profiling of compounds from the infusion of Byrsonima fagifolia Niedenzu. Rapid Communications in Mass Spectrometry, v. 21, p. 1393-1400, 2007. (02/05503-6)

SANNOMIYA, MIRIAM; MONTORO, PAOLA; PIACENTE, SONIA; PIZZA, COSIMO; BRITO, ALBA R. M. S.; VILEGAS, WAGNER. Application of liquid chromatography/electrospray ionization tandem mass spectrometry to the analysis of polyphenolic compounds from an infusion of Byrsonima crassa Niedenzu. Rapid Communications in Mass Spectrometry, v. 19, p. 2244-2250, 2005. (02/05503-6)

HIGUCHI, C. T.; SANNOMIYA, M.; PAVAN, F. R.; LEITE, S. R. A.; SATO, D. N.; FRANZBLAU, S. G.; SACRAMENTO, L. V. S.; VILEGAS, W.; LEITE, C. Q. F.. Byrsonima fagifolia Niedenzu Apolar Compounds with Antitubercular Activity. Evidence-based Complementary and Alternative Medicine, v. 2011, p. 5-pg., 2011-01-01. (02/05503-6)

DE PAULA VASCONCELOS, PAULO CESAR; SEITO, LEONARDO NOBORU; DI STASI, LUIZ CLAUDIO; HIRUMA-LIMA, CLELIA AKIKO; PELLIZZON, CLAUDIA HELENA. Epicatechin Used in the Treatment of Intestinal Inflammatory Disease: An Analysis by Experimental Models. Evidence-based Complementary and Alternative Medicine, 2012. (10/08536-9, 02/05503-6)

SERPELONI, JULIANA MARA; DOS REIS, MARIANA BISARRO; RODRIGUES, JULIANA; DOS SANTOS, LOURDES CAMPANER; VILEGAS, WAGNER; VARANDA, ELIANA A.; DOKKEDAL, ANNE L.; COLUS, ILCE MARA S.. In vivo assessment of DNA damage and protective effects of extracts from Miconia species using the comet assay and micronucleus test. MUTAGENESIS, v. 23, n. 6, p. 501-507, NOV 2008. (02/05503-6)

FLÁVIA CRISTINE MASCIA LOPES; MARISA CAMPOS POLESI PLACERES; CLESO MENDONÇA JORDÃO JUNIOR; CÉLIO TAKASHI HIGUCHI; DANIEL RINALDO; WAGNER VILEGAS; CLARICE QUEICO FUJIMURA LEITE; IRACILDA ZEPPONE CARLOS. Immunological and microbiological activity of Davilla elliptica St. Hill. (Dilleniaceae) against Mycobacterium tuberculosis. Memórias do Instituto Oswaldo Cruz, v. 102, n. 6, p. 769-772, 2007-08-16. (02/05503-6)

RODRIGUES‚ C.M.; RINALDO‚ D.; SANNOMIYA‚ M.; SANTOS‚ L.C.; MONTORO‚ P.; PIACENTE‚ S.; PIZZA‚ C.; VILEGAS‚ W.. High-performance liquid chromatographic separation and identification of polyphenolic compounds from the infusion of Davilla elliptica St. Hill. Phytochemical Analysis, v. 19, n. 1, p. 17-24, 2008. (02/05503-6)

DE PAULA VASCONCELOS, PAULO CESAR; SEITO, LEONARDO NOBORU; DI STASI, LUIZ CLAUDIO; HIRUMA-LIMA, CLELIA AKIKO; PELLIZZON, CLAUDIA HELENA. Epicatechin Used in the Treatment of Intestinal Inflammatory Disease: An Analysis by Experimental Models. Evidence-based Complementary and Alternative Medicine, v. 2012, p. 12-pg., 2012-01-01. (02/05503-6, 10/08536-9)