Abstract
The apparent incompatibility between chemical and pharmacological investigation of a plant species can be solved with the strong determination of dealing rationally with the problem. The research groups on Natural Products Chemistry from the IQ-Araraquara-Unesp, on Pharmacology, from IB-Unicamp and 1B-Botucatu-Unesp and on Biological Sciences, from FCF-Araraquara-Unesp have started a collaboration 2 years ago, and have already produced a significative amount of work, with relevant results on the investigation of plant species with anti-ulcer, anti-oxidant, analgesic, anti-inflammatory and antimicrobial activities. hese results arose from an approach that includes ethnobotanical and ethnopharmacological research, pharmacological, microbiological and mutagenicity assays with crude extracts or infusions, chemical screening to determine new chemical classes of compounds, isolation and structural determination of compounds and use the compounds or enriched fractions to determine the possible pharmacological mechanisms of the action involved with the detected properties. This project deals with the continuation of the integrated chemical and pharmacological investigation of plant extracts, this time investigating species that com poses the bioma Cerrado of the State São Paulo, comparatively to the Bioma Cerrado of the State of Tocantins, which it is also under our investigation. To perform the phytochemical step we intend to use the usual chromatographic techniques, mainly those for the analysis of polar substances (GPC, XAD2, DCCC, HSCC, HPLC, etc), and to determine the structure of the isolated compounds through spectrometric methods (NMR, IR, UV, and MS). To evaluate the biological activities, the possibility of toxicicity and genotoxicity effects of each species will be determined. Simultaneously, we will evaluate the activity of the extracts and pure substances (ar enriched fractions) against different experimental models of peptic ulcer disease which operate by distinct mechanism of ulcerogenesis in man... (AU)
Scientific publications
(12)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
HIGUCHI, C. T.;
SANNOMIYA, M.;
PAVAN, F. R.;
LEITE, S. R. A.;
SATO, D. N.;
FRANZBLAU, S. G.;
SACRAMENTO, L. V. S.;
VILEGAS, W.;
LEITE, C. Q. F.
Byrsonima fagifolia Niedenzu Apolar Compounds with Antitubercular Activity.
Evidence-based Complementary and Alternative Medicine,
2011.
Web of Science Citations: 5.
CARLI, CAMILA B. DE A.;
DE MATOS, DJAMILE C.;
LOPES, FLAVIA C. M.;
MAIA, DANIELLE C. G.;
DIAS, MARISTELA B.;
SANNOMIYA, MIRIAM;
RODRIGUES, CLENILSON M.;
ANDREO, MARCIO A.;
VILEGAS, WAGNER;
COLOMBO, LUCAS L.;
CARLOS, IRACILDA Z.
Isolated Flavonoids against Mammary Tumour Cells LM2.
ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES,
v. 64,
n. 1-2,
p. 32-36,
JAN-FEB 2009.
Web of Science Citations: 12.
SERPELONI, JULIANA MARA;
DOS REIS, MARIANA BISARRO;
RODRIGUES, JULIANA;
DOS SANTOS, LOURDES CAMPANER;
VILEGAS, WAGNER;
VARANDA, ELIANA A.;
DOKKEDAL, ANNE L.;
COLUS, ILCE MARA S.
In vivo assessment of DNA damage and protective effects of extracts from Miconia species using the comet assay and micronucleus test.
MUTAGENESIS,
v. 23,
n. 6,
p. 501-507,
NOV 2008.
Web of Science Citations: 28.
RODRIGUES, CLENILSON M.;
RINALDO, DANIEL;
SANTOS, LOURDES C. DOS;
MONTORO, PAOLA;
PIACENTE, SONIA;
PIZZA, COSIMO;
HIRUMA-LIMA, CLÉLIA A.;
BRITO, ALBA R. M. SOUZA;
VILEGAS, WAGNER.
Metabolic fingerprinting using direct flow injection electrospray ionization tandem mass spectrometry for the characterization of proanthocyanidins from the barks of Hancornia speciosa.
Rapid Communications in Mass Spectrometry,
v. 21,
n. 12,
p. 1907-1914,
June 2007.
SANNOMIYA, MIRIAM;
SANTOS, LOURDES CAMPANER DOS;
CARBONE, VIRGINIA;
NAPOLITANO, ASSUNTA;
PIACENTE, SONIA;
PIZZA, COSIMO;
SOUZA-BRITO, ALBA R. M.;
VILEGAS, WAGNER.
Liquid chromatography/electrospray ionization tandem mass spectrometry profiling of compounds from the infusion of Byrsonima fagifolia Niedenzu.
Rapid Communications in Mass Spectrometry,
v. 21,
p. 1393-1400,
2007.
HIRUMA-LIMA, CLÉLIA AKIKO;
CALVO, TAMARA ROBERTA;
RODRIGUES, CLENILSON MARTINS;
ANDRADE, FÁBIO DONIZETE PEZZUTO;
VILEGAS, WAGNER;
BRITO, ALBA REGINA MONTEIRO SOUZA.
Antiulcerogenic activity of Alchornea castaneaefolia: Effects on somatostatin, gastrin and prostaglandin.
Journal of Ethnopharmacology,
v. 104,
n. 1/2,
p. 215-224,
Mar. 2006.