Research Grants 18/05038-0 - Transtornos relacionados ao uso de substâncias, Consumo de bebidas alc - BV FAPESP
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Effect of environmental enrichment on resilience to chronic unpredictable stress: epigenetic regulation of the BDNF gene and consequences on ethanol consumption

Grant number: 18/05038-0
Support Opportunities:Regular Research Grants
Start date: September 01, 2018
End date: August 31, 2021
Field of knowledge:Biological Sciences - Pharmacology - Neuropsychopharmacology
Principal Investigator:Rosana Camarini
Grantee:Rosana Camarini
Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Environmental conditions exert a great influence on the behavioral and neurochemical effects induced by drugs of abuse. Stress plays an important role in the development of dependence, as well as in relapse. On the other hand, the exposure of rodents to stimuli such as toys, exercise wheels and fellowship with other individuals, a model known as environmental enrichment (EA), is capable of producing beneficial effects to the individual and preventing or reversing pathological conditions, including behavioral/neurochemical effects induced by drugs of abuse. Data from our laboratory demonstrated that EA decreased alcohol consumption in animals exposed to acute constraint stress (Marianno et al., 2017) and decreased the anxiogenic effect induced by acute stress, mediated by a reduction in the nuclear translocation of glucocorticoid (Novaes et al., 2017). In our previous study, we showed that EA prevented and reversed the development of behavioral sensitization to ethanol (Rueda et al., 2012) and decreased the concentrations of BDNF (Brain-Derived Neurotrophic Factor) in the prefrontal cortex of animals submitted to EA, suggesting a possible role of this neurotrophic factor in the neurochemical mechanisms induced by EA (Rueda et al., 2012). In addition, we also find alterations in the expression of microRNAs that are controlled by neurotrophic factors, such as BDNF, in animals that did not express behavioral sensitization to ethanol after EA exposure (Rueda, 2017). Several molecules are commonly involved in the signaling of dependence and stress induced neuroadaptation, such as corticotrophin releasing factor (CRF) and BDNF. Relapses due to emotional states and stress play an important role in the motivation to maintain alcohol consumption, as stress axis deregulation strengthens the search for the drug. The mechanisms by which AD induces its effects on stress resilience and to dependence are not elucidated.Several environmental stimuli lead to epigenetic control of Bdnf expression. Variations in BDNF concentrations occurring between individuals in response to a stimulus or pharmacological treatment may result not only from genetic polymorphism but also from altered epigenetic programming. This project aims to demonstrate the involvement of AD in the stress resilience promoted by AD and the role of epigenetic modifications in the BDNF gene in the prefrontal cortex; besides evaluating the HPA axis participation in this process, through the expression of the nuclear and cytosolic glucocorticoid receptor and inhibition of the HPA axis. The second part of the project aims to study the effects of EA on ethanol consumption in animals submitted to chronic unpredictable stress. (AU)

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Scientific publications (9)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
HOFFMANN, LUCAS B.; RAE, MARIANA; MARIANNO, PRISCILA; PANG, TERENCE Y.; HANNAN, ANTHONY J.; CAMARINI, ROSANA. Preconceptual paternal environmental stimulation alters behavioural phenotypes and adaptive responses intergenerationally in Swiss mice. Physiology & Behavior, v. 223, . (18/05038-0, 17/11987-1, 15/02397-0)
SANTOS, ROGERIO A.; RAE, MARIANA; DARTORA, VANESSA F. M. C.; MATOS, JENYFFER K. R.; CAMARINI, ROSANA; LOPES, LUCIANA B.. Bioresponsive nanostructured systems for sustained naltrexone release and treatment of alcohol use disorder: Development and biological evaluation. International Journal of Pharmaceutics, v. 585, . (17/04174-4, 18/05038-0, 18/13877-1, 13/16617-7, 18/18813-1, 15/02397-0)
CARRARA-NASCIMENTO, PRISCILA FERNANDES; HOFFMANN, LUCAS BARBOSA; FLORIO, JORGE CAMILO; PLANETA, CLEOPATRA SILVA; CAMARINI, ROSANA. Effects of Ethanol Exposure During Adolescence or Adulthood on Locomotor Sensitization and Dopamine Levels in the Reward System. FRONTIERS IN BEHAVIORAL NEUROSCIENCE, v. 14, . (12/17228-1, 15/02397-0, 12/10260-7, 17/11987-1, 18/05038-0)
COSTA, GABRIEL ARAUJO; DE GUSMAO TAVEIROS SILVA, NIVEA KARLA; MARIANNO, PRISCILA; CHIVERS, PRITI; BAILEY, ALEXIS; CAMARINI, ROSANA. Environmental Enrichment Increased Bdnf Transcripts in the Prefrontal Cortex: Implications for an Epigenetically Controlled Mechanism. Neuroscience, v. 526, p. 13-pg., . (18/05038-0)
KIMURA, LOUISE F.; NOVAES, LEONARDO S.; PICOLO, GISELE; MUNHOZ, CAROLINA D.; CHEUNG, CHI W.; CAMARINI, ROSANA. How environmental enrichment balances out neuroinflammation in chronic pain and comorbid depression and anxiety disorders. British Journal of Pharmacology, . (19/05882-8, 18/05038-0)
CONTO, MARCOS BRANDAO; DOS SANTOS, NILTON BARRETO; MUNHOZ, CAROLINA DEMARCHI; MARCOURAKIS, TANIA; D'ALMEIDA, VANIA; CAMARINI, ROSANA. Exposure to Running Wheels Prevents Ethanol Rewarding Effects: The Role of CREB and Deacetylases SIRT-1 and SIRT-2 in the Nucleus Accumbens and Prefrontal Cortex. Neuroscience, v. 469, p. 125-137, . (18/05038-0, 12/09898-7)
CONTO, MARCOS BRANDAO; PAUTASSI, RICARDO MARCOS; CAMARINI, ROSANA. Rewarding and Antidepressant Properties of Ketamine and Ethanol: Effects on the Brain-Derived Neurotrophic Factor and TrkB and p75(NTR) Receptors. Neuroscience, v. 493, p. 14-pg., . (12/09898-7, 18/05038-0)
KIMURA, LOUISE F.; NOVAES, LEONARDO S.; PICOLO, GISELE; MUNHOZ, CAROLINA D.; CHEUNG, CHI W.; CAMARINI, ROSANA. How environmental enrichment balances out neuroinflammation in chronic pain and comorbid depression and anxiety disorders. British Journal of Pharmacology, v. 179, n. 8, p. 21-pg., . (19/05882-8, 18/05038-0)
RAE, MARIANA; LEMOS DUARTE, MARIANA; GOMES, IVONE; CAMARINI, ROSANA; DEVI, LAKSHMI A.. Oxytocin and vasopressin: Signalling, behavioural modulation and potential therapeutic effects. British Journal of Pharmacology, v. 179, n. 8, p. 21-pg., . (18/05038-0)