Photobiomodulation with light-emitting diode (LED) can improve the efficiency of mesenquimial stem cell therapy in post-ischemic heart failure?

• Grant number: 18/06865-7
• Support Opportunities: Regular Research Grants
• Duration: October 01, 2018 - June 30, 2021
• Field of knowledge: Biological Sciences - Physiology - Physiology of Organs and Systems
• Principal Investigator: Andrey Jorge Serra
• Grantee: Andrey Jorge Serra
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated researchers: Jose Renato Dias Oliveira Pinto ; Paulo de Tarso Camillo de Carvalho ; Paulo Jose Ferreira Tucci

Abstract

Myocardial infarction causes irreversible loss of cardiomyocytes, culminating in negative cardiac remodeling and heart failure (HF). Considering the limited intrinsic repair ability of myocardial, and a high morbidity and mortality due to HF even with the optimized therapeutic arsenal, searches for more effective alternatives to attenuate the cardiac remodeling post-infarction reigns in the literature. In this regard, mesenchymal stem cells (MSCs) transplantation is a promising approach to restore cardiac function. The MSCs expand easily ex vivo and show differentiation for endothelial cells and cardiomyocytes in vitro. Moreover, the paracrine benefits of MSCs arising from the secretion of growth factors and immunomodulatory (e.g., cytokines). Although experimental and clinical studies have illustrated benefits of MSCs in infarcted hearts, there are no singular findings. Main reasons for these findings are reduced cell adhesion in the organ and cell death linked to inhospitable microenvironment in the infarcted myocardium. Therefore, new approaches are needed to enhance cellular home and to improve the resistance as well as efficiency of CTMs. Using a preclinical myocardial infarction model in rats, we will investigate: 1) the hypothesis that light emitting diode (LED) stimulus can improve the viability and proliferation and, potentiate the secretion of cytokines and growth factors of mesenchymal stem cells of adipose tissue (CTMA). Additionally, we will test different irradiation parameters, targeting to determine the LED dose with more evident biological effects; 2) whether in vitro pre-irradiation with LED improves the therapeutic CTMA action in the infarcted heart. (AU)

Scientific publications

(12)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GRANDINETTI, VANESSA; CARLOS, FERNANDO P. EREIRA; ANTONIO, EDNEI LUIZ; DE OLIVEIRA, HELENITA ANTONIA; NEVES DOS SANTOS, LUIS FELIPE; YOSHIZAKI, AMANDA; DIAS MARTINS MANSANO, BARBARA SAMPAIO; SILVA, FLAVIO ANDRE; PORTE, LESLIE ANDREWS; ALBUQUERQUE-PONTES, GIANNA MOES; et al. Photobiomodulation therapy combined with carvedilol attenuates post-infarction heart failure by suppressing excessive inflammation and oxidative stress in rats. SCIENTIFIC REPORTS, v. 9, JUL 1 2019. (18/06865-7, 15/11028-9)

DIAS MARTINS MANSANO, BARBARA SAMPAIO; DA ROCHA, VITOR POCANI; ANTONIO, EDNEI LUIZ; PERON, DANIELE FERNANDA; DE LIMA, RAFAEL DO NASCIMENTO; FERREIRA TUCCI, PAULO JOSE; SERRA, ANDREY JORGE. Enhancing the Therapeutic Potential of Mesenchymal Stem Cells with Light-Emitting Diode: Implications and Molecular Mechanisms. OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, v. 2021, FEB 3 2021. (18/06865-7)

MANSANO, BARBARA SAMPAIO DIAS MARTINS; DA ROCHA, VITOR POCANI; TEIXEIRA, IGHOR LUIZ AZEVEDO; DE OLIVEIRA, HELENITA ANTONIA; VIEIRA, STELLA SOUZA; ANTONIO, EDNEI LUIZ; TUCCI, PAULO JOSE FERREIRA; SERRA, ANDREY JORGE. Light-emitting Diode Can Enhance the Metabolism and Paracrine Action of Mesenchymal Stem Cells. Photochemistry and Photobiology, v. N/A, p. 9-pg., 2023-03-05. (18/06865-7)

SERRA, ANDREY JORGE; PINTO, JOSE RENATO; PROKIC, MARKO D.; ARSA, GISELA; VASCONSUELO, ANDREA. Oxidative Stress in Muscle Diseases: Current and Future Therapy 2019. OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, v. 2020, p. 4-pg., 2020-04-22. (18/06865-7)

OLIVEIRA, CLAUDIA DE SOUZA; DE OLIVEIRA, HELENITA ANTONIA; AZEVEDO TEIXEIRA, IGHOR LUIZ; ANTONIO, EDNEI LUIZ; SILVA, FLAVIO ANDRE; SUNEMI, SIMONE; LEAL-JUNIOR, ERNESTO CESAR; DE CARVALHO, PAULO DE TARSO CAMILLO; FERREIRA TUCCI, PAULO JOSE; SERRA, ANDREY JORGE. Low-level laser therapy prevents muscle apoptosis induced by a high-intensity resistance exercise in a dose-dependent manner. Lasers in Medical Science, FEB 2020. (18/06865-7)

DE SOUZA VIEIRA, STELLA; DE MELO, BRUNNO LEMES; DOS SANTOS, LUIS FELIPE; CUMMINGS, CHARLES O.; TUCCI, PAULO JOSE FERREIRA; SERRA, ANDREY JORGE. Exercise Training in Boosting Post-Mi Mesenchymal Stem Cell Therapy. STEM CELL REVIEWS AND REPORTS, OCT 2021. (18/06865-7)

DE LIMA, RAFAEL DO NASCIMENTO; VIEIRA, STELLA SOUSA; ANTONIO, EDNEI LUIZ; CAMILLO DE CARVALHO, PAULO DE TARSO; VIEIRA, RODOLFO DE PAULA; DIAS MARTINS MANSANO, BARBARA SAMPAIO; DE ARRUDA JUNIOR, DANIEL FERREIRA; COSTA GIRARDI, ADRIANA CASTELLO; FERREIRA TUCCI, PAULO JOSE; SERRA, ANDREY JORGE. Low-level laser therapy alleviates the deleterious effect of doxorubicin on rat adipose tissue-derived mesenchymal stem cells. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 196, JUL 2019. (18/06865-7, 15/14239-0)

SUNEMI, SIMONE MITIE; TEIXEIRA, IGHOR LUIZ AZEVEDO; MANSANO, BARBARA SAMPAIO DIAS MARTINS; DE OLIVEIRA, HELENITA ANTONIA; ANTONIO, EDNEI LUIZ; DE SOUZA OLIVEIRA, CLAUDIA; LEAL-JUNIOR, ERNESTO CESAR PINTO; TUCCI, PAULO JOSE FERREIRA; SERRA, ANDREY JORGE. Post-resistance exercise photobiomodulation therapy has a more effective antioxidant effect than pre-application on muscle oxidative stress. PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, v. 20, n. 4, APR 2021. (18/06865-7)

PERON, DANIELE; PRATES, RENATO ARAUJO; ANTONIO, EDNEI LUIZ; AZEVEDO TEIXEIRA, IGHOR LUIZ; DE OLIVEIRA, HELENITA ANTONIA; DIAS MARTINS MANSANO, BARBARA SAMPAIO; BERGAMO, ALEXANDRE; ALMEIDA, DIRCEU RODRIGUES; DARIOLLI, RAFAEL; FERREIRA TUCCI, PAULO JOSE; et al. A common oral pathogen Porphyromonas gingivalis induces myocarditis in rats. JOURNAL OF CLINICAL PERIODONTOLOGY, v. 49, n. 5, p. 12-pg., 2022-02-27. (18/06865-7)

VIEIRA, STELLA SOUZA; ANTONIO, EDNEI LUIZ; DE MELO, BRUNNO LEMES; NEVES DOS SANTOS, LUIS FELIPE; SANTANA, EDUARDO TADEU; FELICIANO, REGIANE; NAVARRO MARQUES, FABIO LUIZ; FARIA, DANIELE DE PAULA; BUCHPIGUEL, CARLOS ALBERTO; SILVA JR, JOSE ANTONIO; et al. Increased Myocardial Retention of Mesenchymal Stem Cells Post-MI by Pre-Conditioning Exercise Training. STEM CELL REVIEWS AND REPORTS, v. 16, n. 4, APR 2020. (09/54225-8, 18/06865-7, 15/11028-9)

VIEIRA, STELLA SOUZA; ANTONIO, EDNEI LUIZ; DE MELO, BRUNNO LEMES; NEVES DOS SANTOS, LUIS FELIPE; SANTANA, EDUARDO TADEU; FELICIANO, REGIANE; NAVARRO MARQUES, FABIO LUIZ; FARIA, DANIELE DE PAULA; BUCHPIGUEL, CARLOS ALBERTO; SILVA JR, JOSE ANTONIO; et al. Increased Myocardial Retention of Mesenchymal Stem Cells Post-MI by Pre-Conditioning Exercise Training. STEM CELL REVIEWS AND REPORTS, v. 16, n. 4, p. 12-pg., 2020-04-18. (18/06865-7, 09/54225-8, 15/11028-9)

OLIVEIRA, CLAUDIA DE SOUZA; DE OLIVEIRA, HELENITA ANTONIA; AZEVEDO TEIXEIRA, IGHOR LUIZ; ANTONIO, EDNEI LUIZ; SILVA, FLAVIO ANDRE; SUNEMI, SIMONE; LEAL-JUNIOR, ERNESTO CESAR; DE CARVALHO, PAULO DE TARSO CAMILLO; FERREIRA TUCCI, PAULO JOSE; SERRA, ANDREY JORGE. Low-level laser therapy prevents muscle apoptosis induced by a high-intensity resistance exercise in a dose-dependent manner. Lasers in Medical Science, v. 35, n. 8, p. 4-pg., 2020-02-06. (18/06865-7)