Photosensitization of oropharyngeal (HEp-2) cancer cells induced by the action of ...
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Uncovering the PDT mechanism of new organic/inorganic hybrid photosensitizers
| Grant number: | 17/50412-4 |
| Support type: | Regular Research Grants |
| Duration: | October 01, 2018 - September 30, 2020 |
| Field of knowledge: | Biological Sciences - Biophysics |
| Cooperation agreement: | Leiden University |
| Mobility Program: | SPRINT - Projetos de pesquisa - Mobilidade |
| Principal Investigator: | Mauricio da Silva Baptista |
| Grantee: | Mauricio da Silva Baptista |
| Principal investigator abroad: | Sylvestre Bonnet |
| Institution abroad: | Universiteit Leiden, Netherlands |
| Home Institution: | Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil |
| Associated research grant: | 13/07937-8 - Redoxome - Redox Processes in Biomedicine, AP.CEPID |
Abstract
The use of light as a therapeutic modality has recently become an attractive mode of therapy due to the development of efficient and affordable light sources, together with its low invasiveness and low systemic toxicity. Consequently, photo-activated drugs (photosensitizers-PS), are playing key roles in the present clinical portfolio (in a modality called Photodynamic Therapy-PDT). Although the majority of PDT drugs available are based in organic compounds the amount of research dedicated to the development of photosensitizers based in inorganic/organic hybrids has increased recently. Several groups have shown improved efficiency, probably because of a multimode of action but the mechanistic works are still missing. The major aim of this proposal is to bring together the expertise from the Bonnet and Baptista groups for the design and development of new ruthenium-based photosensitizers tailored for the maximizing photosensitizer efficiency by modulating type I and type II mechanisms. The specific aims of this proposal are: i) synthesis and characterization of new inorganic/organic hybrid complexes; ii) Quantification of the ability of the complexes to undergo photoinduced sensitization of singlet oxygen and type I reaction in solution and membrane mimic; iii)Testing in cell culture (monolayer and 3D). (AU)