Bee-agriculture interactions: perspectives to sustainable use

• Grant number: 17/21097-3
• Support type: Research Projects - Thematic Grants
• Duration: November 01, 2018 - October 31, 2023
• Field of knowledge: Biological Sciences - Zoology
• Principal Investigator: Osmar Malaspina
• Grantee: Osmar Malaspina
• Home Institution: Centro de Estudos de Insetos Sociais (CEIS). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil
• Co-Principal Investigators: Bruno Campos Janegitz ; Elaine Cristina Mathias da Silva Zacarin ; Ricardo Ferraz de Oliveira ; Roberta Cornélio Ferreira Nocelli ; Thaisa Cristina Roat
• Assoc. researchers: Cláudia Inês da Silva ; Patrícia Andrea Monquero
• Associated scholarship(s): 19/24428-6 - Development of Pd and Ag binary materials for modification of the glassy carbon electrode and disposable printed electrode for melatonin and nitrite determination, BP.IC; 19/23177-0 - Development of disposable devices with conductive inks and low cost substrates for the biosensing of hormonal dysfunctions and detection of Malaria, BP.DD; 19/23342-0 - Development of novel immunosensor architechtures for the deterction of Huntington and Parkinson Diseases biomarkers, BP.DD; 19/20109-3 - Metabolic route of pesticides in bees, BP.PD; 19/01844-4 - Development of nanostructured electrodes for determination of agrotoxic residues in honey, BP.PD

Abstract

Bees play a key role in pollinating services in natural ecosystems and agroecosystems. However, this indispensable service is due primarily to deforestation, fragmentation of habitat and loss of natural areas, which are replaced by urban centers, Agriculture and Livestock. In addition, subsequent activities related to Agriculture in the conventional system, such as the indiscriminate and incorrect use of agrochemicals, have contributed significantly to the mortality of native bees and more massively disseminated species of the exotic species Apis mellifera. During the collection of food resources (pollen, nectar, floral oils and resins) bees come into contact with pesticides. Whether directly or systemically via the plants, this contact with pesticides has caused lethal and sublethal effects on honey bees, and little or nothing is known about the effects on native and non-social native bees. Our general objective is to evaluate the effects of different pesticides and their mixtures on A. mellifera, considered as a model species in toxicological studies, and compare these effects with two species of native bees, Melipona scutellaris and Scaptotrigona postica; a parasocial species, Xylocopa frontalis e; two solitary species, Tetrapedia diversipes and Centris analis. The specific objectives related to the subprojects are: 1) to analyze the biological responses (endpoints) to the agrochemicals tested through laboratory bioassays; 2) to evaluate the detection of agrochemicals and hydrogen peroxide in honey using nanostructured thin films produced from beeswax; 3) to verify the effect of pesticides on plant-insect interaction by recording the electrical activity of plants. (AU)

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BRAZACA, LAIS CANNIATTI; SAMPAIO, ISABELLA; ZUCOLOTTO, VALTENCIR; JANEGITZ, BRUNO CAMPOS. Applications of biosensors in Alzheimer's disease diagnosis. Talanta, v. 210, APR 1 2020. Web of Science Citations: 0.

MAURUTO DE OLIVEIRA, GABRIELA C.; DE SOUZA CARVALHO, JEFFERSON HENRIQUE; BRAZACA, LAIS CANNIATTI; SILVA VIEIRA, NIRTON CRISTI; JANEGITZ, BRUNO CAMPOS. Flexible platinum electrodes as electrochemical sensor and immunosensor for Parkinson's disease biomarkers. BIOSENSORS & BIOELECTRONICS, v. 152, MAR 15 2020. Web of Science Citations: 0.

KALINKE, CRISTIANE; NEUMSTEIR, NAILE VACILOTTO; APARECIDO, GABRIEL DE OLIVEIRA; DE BARROS FERRAZ, THIAGO VASCONCELOS; DOS SANTOS, PAMYLA LAYENE; JANEGITZ, BRUNO CAMPOS; BONACIN, JULIANO ALVES. Comparison of activation processes for 3D printed PLA-graphene electrodes: electrochemical properties and application for sensing of dopamine. ANALYST, v. 145, n. 4, p. 1207-1218, FEB 21 2020. Web of Science Citations: 0.

PRADEL-FILHO, LAURO A.; ANDREOTTI, ISABELA A. A.; CARVALHO, JEFFERSON H. S.; ARAUJO, DIELE A. G.; ORZARI, LUIZ O.; GATTI, ALEXANDRE; TAKEUCHI, REGINA M.; SANTOS, ANDRE L.; JANEGITZ, BRUNO C. Glass varnish-based carbon conductive ink: A new way to produce disposable electrochemical sensors. SENSORS AND ACTUATORS B-CHEMICAL, v. 305, FEB 15 2020. Web of Science Citations: 0.

CAMARGO, JESSICA R.; ANDREOTTI, ISABELLA A. A.; KALINKE, CRISTIANE; HENRIQUE, JULIA M.; BONACIN, JULIANO A.; JANEGITZ, BRUNO C. Waterproof paper as a new substrate to construct a disposable sensor for the, electrochemical determination of paracetamol and melatonin. Talanta, v. 208, FEB 1 2020. Web of Science Citations: 0.

AZEVEDO, PATRICIA; BUTOLO, NICOLE PAVAN; DE ALENCAR, LUCIANO DELMONDES; SOARES-LIMA, HELLEN MARIA; SALES, VICTOR RIBEIRO; MALASPINA, OSMAR; FERREIRA NOCELLI, ROBERTA CORNELIO. Standardization of in vitro nervous tissue culture for honeybee: A high specificity toxicological approach. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, v. 189, FEB 2020. Web of Science Citations: 0.

VIDAL RAMOS, MATHEUS MESQUITA; CARVALHO, JEFFERSON H. S.; DE OLIVEIRA, PAULO ROBERTO; JANEGITZ, BRUNO CAMPOS. Determination of serotonin by using a thin film containing graphite, nanodiamonds and gold nanoparticles anchored in casein. MEASUREMENT, v. 149, JAN 2020. Web of Science Citations: 0.

GRELLA, TATIANE CAROLINE; SOARES-LIMA, HELLEN MARIA; MALASPINA, OSMAR; FERREIRA NOCELLI, ROBERTA CORNELIO. Semi-quantitative analysis of morphological changes in bee tissues: A toxicological approach. Chemosphere, v. 236, DEC 2019. Web of Science Citations: 2.

ZAMBIANCO, NAIARA ALANA; SILVA, TIAGO ALMEIDA; ZANIN, HUDSON; FATIBELLO-FILHO, ORLANDO; JANEGITZ, BRUNO CAMPOS. Novel electrochemical sensor based on nanodiamonds and manioc starch for detection of diquat in environmental samples. DIAMOND AND RELATED MATERIALS, v. 98, OCT 2019. Web of Science Citations: 0.

TADEI, RAFAELA; DOMINGUES, CAIO E. C.; MALAQUIAS, JOSE BRUNO; CAMILO, ERASNILSON VIEIRA; MALASPINA, OSMAR; SILVA-ZACARIN, ELAINE C. M. Late effect of larval co-exposure to the insecticide clothianidin and fungicide pyraclostrobin in Africanized Apis mellifera. SCIENTIFIC REPORTS, v. 9, MAR 1 2019. Web of Science Citations: 4.