Bee-agriculture interactions: perspectives to sustainable use

• Grant number: 17/21097-3
• Support type: Research Projects - Thematic Grants
• Duration: November 01, 2018 - October 31, 2023
• Field of knowledge: Biological Sciences - Zoology - Applied Zoology
• Principal researcher: Osmar Malaspina
• Grantee: Osmar Malaspina
• Home Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil
• Principal researchers: Bruno Campos Janegitz ; Bruno Campos Janegitz ; Elaine Cristina Mathias da Silva Zacarin ; Ricardo Ferraz de Oliveira ; Roberta Cornélio Ferreira Nocelli ; Thaisa Cristina Roat
• Assoc. researchers: Cláudia Inês da Silva ; Patrícia Andrea Monquero
• Associated scholarship(s): 21/01359-9 - Effects of the insecticide thiamethoxam on Scaptotrigona postica Latreille, 1807 (Hymenoptera, Meliponina): determination of the average lethal concentration and analysis of enzyme activity, BP.IC; 20/12639-0 - Effects of the neonicotinoid insecticide Imidacloprid and the fungicide Pyraclostrobin on the solitary bee Tetrapedia diversipes: evaluation of energy metabolism, cellular responses and composition of the microbiome, BP.PD; 20/13455-0 - Characterization of tomato electrical activity in interaction with social, parasocial and solitary bees after exposure to neonicotinoids, BP.PD; + associated scholarships 20/03527-3 - Differential gene expression of Melipona scutellaris Malpighi tubule transcriptomes exposed to thiamethoxam, BP.MS; 19/27863-5 - Can pesticide mixing harm the health, behavior and reproduction of native solitary bees?, BP.DR; 18/24245-6 - Genomic, transcriptomic and metagenomic in populations of native stingless bees aiming the search for detoxification genes, BP.DR; 19/23177-0 - Development of disposable devices with conductive inks and low cost substrates for the biosensing of hormonal dysfunctions and detection of Malaria, BP.DD; 19/23342-0 - Development of novel immunosensor architechtures for the deterction of Huntington and Parkinson Diseases biomarkers, BP.DD; 19/24428-6 - Development of Pd and Ag binary materials for modification of the glassy carbon electrode and disposable printed electrode for melatonin and nitrite determination, BP.IC; 19/20109-3 - Metabolic route of pesticides in bees, BP.PD; 19/01844-4 - Development of nanostructured electrodes for determination of agrotoxic residues in honey, BP.PD - associated scholarships

Abstract

Bees play a key role in pollinating services in natural ecosystems and agroecosystems. However, this indispensable service is due primarily to deforestation, fragmentation of habitat and loss of natural areas, which are replaced by urban centers, Agriculture and Livestock. In addition, subsequent activities related to Agriculture in the conventional system, such as the indiscriminate and incorrect use of agrochemicals, have contributed significantly to the mortality of native bees and more massively disseminated species of the exotic species Apis mellifera. During the collection of food resources (pollen, nectar, floral oils and resins) bees come into contact with pesticides. Whether directly or systemically via the plants, this contact with pesticides has caused lethal and sublethal effects on honey bees, and little or nothing is known about the effects on native and non-social native bees. Our general objective is to evaluate the effects of different pesticides and their mixtures on A. mellifera, considered as a model species in toxicological studies, and compare these effects with two species of native bees, Melipona scutellaris and Scaptotrigona postica; a parasocial species, Xylocopa frontalis e; two solitary species, Tetrapedia diversipes and Centris analis. The specific objectives related to the subprojects are: 1) to analyze the biological responses (endpoints) to the agrochemicals tested through laboratory bioassays; 2) to evaluate the detection of agrochemicals and hydrogen peroxide in honey using nanostructured thin films produced from beeswax; 3) to verify the effect of pesticides on plant-insect interaction by recording the electrical activity of plants. (AU)

Scientific publications

(33)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

KALINKE, CRISTIANE; DE OLIVEIRA, PAULO R.; BONACIN, JULIANO A.; JANEGITZ, BRUNO C.; MANGRICH, ANTONIO S.; MARCOLINO-JUNIOR, LUIZ H.; BERGAMINI, MARCIO F. State-of-the-art and perspectives in the use of biochar for electrochemical and electroanalytical applications. GREEN CHEMISTRY, JUN 2021. Web of Science Citations: 0.

BRAZACA, LAIS CANNIATTI; DOS SANTOS, PAMYLA LAYENE; DE OLIVEIRA, PAULO ROBERTO; ROCHA, DIEGO PESSOA; STEFANO, JESSICA SANTOS; KALINKE, CRISTIANE; MUNOZ, RODRIGO ALEJANDRO ABARZA; BONACIN, JULIANO ALVES; JANEGITZ, BRUNO CAMPOS; CARRILHO, EMANUEL. Biosensing strategies for the electrochemical detection of viruses and viral diseases e A review. Analytica Chimica Acta, v. 1159, MAY 15 2021. Web of Science Citations: 0.

CAMARGO, JESSICA ROCHA; ORZARI, LUIZ OTAVIO; ARAUJO, DIELE APARECIDA GOUVEIA; DE OLIVEIRA, PAULO ROBERTO; KALINKE, CRISTIANE; ROCHA, DIEGO PESSOA; DOS SANTOS, ANDRE LUIZ; TAKEUCHI, REGINA MASSAKO; MUNOZ, RODRIGO ALEJANDRO ABARZA; BONACIN, JULIANO ALVES; JANEGITZ, BRUNO CAMPOS. Development of conductive inks for electrochemical sensors and biosensors. Microchemical Journal, v. 164, MAY 2021. Web of Science Citations: 0.

PACHU, JESSICA K. S.; MACEDO, FRANCYNES C. O.; MALAQUIAS, JOSE B.; RAMALHO, FRANCISCO S.; OLIVEIRA, RICARDO F.; FRANCO, FLAVIA PEREIRA; GODOY, WESLEY A. C. Electrical signalling on Bt and non-Bt cotton plants under stress by Aphis gossypii. PLoS One, v. 16, n. 4 APR 8 2021. Web of Science Citations: 0.

DOS SANTOS, FABRICIO A.; VIEIRA, NIRTON C. S.; ZAMBIANCO, NAIARA A.; JANEGITZ, BRUNO C.; ZUCOLOTTO, VALTENCIR. The layer-by-layer assembly of reduced graphene oxide films and their application as solution-gated field-effect transistors. Applied Surface Science, v. 543, MAR 30 2021. Web of Science Citations: 0.

FERNANDES-JUNIOR, WILSON SILVA; ZACCARIN, LETICIA FERNANDA; OLIVEIRA, GEISER GABRIEL; DE OLIVEIRA, PAULO ROBERTO; KALINKE, CRISTIANE; BONACIN, JULIANO ALVES; PRAKASH, JAI; JANEGITZ, BRUNO CAMPOS. Electrochemical Sensor Based on Nanodiamonds and Manioc Starch for Detection of Tetracycline. JOURNAL OF SENSORS, v. 2021, MAR 9 2021. Web of Science Citations: 0.

GUSTANI, FLAVIA MARIA; SILVA, TIAGO ALMEIDA; CAMARGO, JESSICA ROCHA; DE SOUZA CARVALHO, JEFFERSON HENRIQUE; JANEGITZ, BRUNO CAMPOS; FUJIHARA, RICARDO TOSHIO. Synthesis, Attractiveness and Effectiveness of Chitosan-Tapioca Encapsulates in Atta Sexdens (Hymenoptera: Formicidae). JOURNAL OF POLYMERS AND THE ENVIRONMENT, FEB 2021. Web of Science Citations: 0.

KALINKE, CRISTIANE; NEUMSTEIR, NAILE VACIONOTTO; DE OLIVEIRA, PAULO ROBERTO; JANEGITZ, BRUNO CAMPOS; BONACIN, JULIANO ALVES. Sensing of L-methionine in biological samples through fully 3D-printed electrodes. Analytica Chimica Acta, v. 1142, p. 135-142, JAN 15 2021. Web of Science Citations: 0.

PACHU, JESSICA K. S.; MACEDO, FRANCYNES C. O.; DA SILVA, FABIA B.; MALAQUIAS, JOSE B.; RAMALHO, FRANCISCO S.; OLIVEIRA, RICARDO F.; GODOY, WESLEY A. C. Imidacloprid-mediated stress on non-Bt and Bt cotton, aphid and ladybug interaction: Approaches based on insect behaviour, fluorescence, dark respiration and plant electrophysiology. Chemosphere, v. 263, JAN 2021. Web of Science Citations: 0.

DA COSTA DOMINGUES, CAIO EDUARDO; BELLO INOUE, LAIS VIEIRA; MATHIAS DA SILVA-ZACARIN, ELAINE CRISTINA; MALASPINA, OSMAR. Fungicide pyraclostrobin affects midgut morphophysiology and reduces survival of Brazilian native stingless bee Melipona scutellaris. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, v. 206, DEC 15 2020. Web of Science Citations: 2.

SILVA, VINICIUS A. O. P.; FERNANDES-JUNIOR, WILSON S.; ROCHA, DIEGO P.; STEFANO, JESSICA S.; MUNOZ, RODRIGO A. A.; BONACIN, JULIANO A.; JANEGITZ, BRUNO C. 3D-printed reduced graphene oxide/polylactic acid electrodes: A new prototyped platform for sensing and biosensing applications. BIOSENSORS & BIOELECTRONICS, v. 170, DEC 15 2020. Web of Science Citations: 1.

RAYMUNDO-PEREIRA, PAULO A.; SILVA, TIAGO A.; CAETANO, FABIO R.; RIBOVSKI, LAIS; ZAPP, EDUARDO; BRONDANI, DANIELA; BERGAMINI, MARCIO F.; MARCOLINO, JR., LUIZ H.; BANKS, CRAIG E.; OLIVEIRA, JR., OSVALDO N.; JANEGITZ, BRUNO C.; FATIBELLO-FILHO, ORLANDO. Polyphenol oxidase-based electrochemical biosensors: A review. Analytica Chimica Acta, v. 1139, p. 198-221, DEC 1 2020. Web of Science Citations: 2.

OLIVEIRA, GEISER GABRIEL; AZZI, DEBORAH CHRISTINE; SILVA, TIAGO ALMEIDA; DE OLIVEIRA, PAULO ROBERTO; FATIBELLO-FILHO, ORLANDO; JANEGITZ, BRUNO CAMPOS. Sensitive Voltammetric Detection of Chloroquine Drug by Applying a Boron-Doped Diamond Electrode. C-JOURNAL OF CARBON RESEARCH, v. 6, n. 4 DEC 2020. Web of Science Citations: 0.

ZAMBIANCO, NAIARA A.; DA SILVA, VINICIUS A. O. P.; ORZARI, LUIZ O.; CORAT, EVALDO J.; ZANIN, HUDSON G.; SILVA, TIAGO A.; BULLER, GARY A.; KEEFE, EDMUND M.; BANKS, CRAIG E.; JANEGITZ, BRUNO C. Determination of tadalafil in pharmaceutical samples by vertically oriented multi-walled carbon nanotube electrochemical sensing device. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, v. 877, NOV 15 2020. Web of Science Citations: 0.

ROSA-FONTANA, ANNELISE DE SOUZA; DORIGO, ADNA SUELEN; SOARES-LIMA, HELLEN MARIA; FERREIRA NOCELLI, ROBERTA CORNELIO; MALASPINA, OSMAR. Is the Water Supply a Key Factor in Stingless Bees' Intoxication?. JOURNAL OF INSECT SCIENCE, v. 20, n. 6 NOV 12 2020. Web of Science Citations: 0.

DA COSTA DOMINGUES, CAIO EDUARDO; BELLO INOUE, LAIS VIEIRA; MATHIAS DA SILVA-ZACARIN, ELAINE CRISTINA; MALASPINA, OSMAR. Foragers of Africanized honeybee are more sensitive to fungicide pyraclostrobin than newly emerged bees. Environmental Pollution, v. 266, n. 2 NOV 2020. Web of Science Citations: 2.

ARAUJO, DIELE A. G.; CAMARGO, JESSICA R.; PRADELA-FILHO, LAURO A.; LIMA, ANA P.; MUNOZ, RODRIGO A. A.; TAKEUCHI, REGINA M.; JANEGITZ, BRUNO C.; SANTOS, ANDRE L. A lab-made screen-printed electrode as a platform to study the effect of the size and functionalization of carbon nanotubes on the voltammetric determination of caffeic acid. Microchemical Journal, v. 158, NOV 2020. Web of Science Citations: 4.

OLIANI PEDRO DA SILVA, VINICIUS APARECIDO; DE FREITAS, RAFAELA CRISTINA; DE OLIVEIRA, PAULO ROBERTO; MOREIRA, ROGER CARDOSO; MARCOLINO-JUNIOR, LUIZ HUMBERTO; BERGAMINI, MARCIO FERNANDO; COLTRO, WENDELL K. T.; JANEGITZ, BRUNO CAMPOS. Microfluidic paper-based device integrated with smartphone for point-of-use colorimetric monitoring of water quality index. MEASUREMENT, v. 164, NOV 2020. Web of Science Citations: 0.

DE SOUZA, DANIEL CARDOSO; ORZARI, LUIZ OTAVIO; DE OLIVEIRA, PAULO ROBERTO; KALINKE, CRISTIANE; BONACIN, JULIANO ALVES; MALASPINA, OSMAR; FERREIRA NOCELLI, ROBERTA CORNELIO; JANEGITZ, BRUNO CAMPOS. Electrochemical Sensor Based on Beeswax and Carbon Black Thin Biofilms for Determination of Paraquat in Apis mellifera Honey. FOOD ANALYTICAL METHODS, v. 14, n. 3 OCT 2020. Web of Science Citations: 0.

ROSA-FONTANA, ANNELISE; DORIGO, ADNA SUELEN; GALASCHI-TEIXEIRA, JULIANA STEPHANIE; NOCELLI, ROBERTA C. F.; MALASPINA, OSMAR. What is the most suitable native bee species from the Neotropical region to be proposed as model-organism for toxicity tests during the larval phase?. Environmental Pollution, v. 265, n. B OCT 2020. Web of Science Citations: 0.

BUTOLO, NICOLE PAVAN; AZEVEDO, PATRICIA; DE ALENCAR, LUCIANO DELMONDES; DOMINGUES, CAIO E. C.; MIOTELO, LUCAS; MALASPINA, OSMAR; FERREIRA NOCELLI, ROBERTA CORNELIO. A high quality method for hemolymph collection from honeybee larvae. PLoS One, v. 15, n. 6 JUN 18 2020. Web of Science Citations: 0.

DE OLIVEIRA, GABRIELA CAROLINA MAURUTO; CAMARGO, JESSICA ROCHA; VIEIRA, NIRTON CRISTI SILVA; JANEGITZ, BRUNO CAMPOS. A new disposable electrochemical sensor on medical adhesive tape. Journal of Solid State Electrochemistry, v. 24, n. 10 JUN 2020. Web of Science Citations: 0.

BRAZACA, LAIS CANNIATTI; SAMPAIO, ISABELLA; ZUCOLOTTO, VALTENCIR; JANEGITZ, BRUNO CAMPOS. Applications of biosensors in Alzheimer's disease diagnosis. Talanta, v. 210, APR 1 2020. Web of Science Citations: 0.

MAURUTO DE OLIVEIRA, GABRIELA C.; DE SOUZA CARVALHO, JEFFERSON HENRIQUE; BRAZACA, LAIS CANNIATTI; SILVA VIEIRA, NIRTON CRISTI; JANEGITZ, BRUNO CAMPOS. Flexible platinum electrodes as electrochemical sensor and immunosensor for Parkinson's disease biomarkers. BIOSENSORS & BIOELECTRONICS, v. 152, MAR 15 2020. Web of Science Citations: 0.

KALINKE, CRISTIANE; NEUMSTEIR, NAILE VACILOTTO; APARECIDO, GABRIEL DE OLIVEIRA; DE BARROS FERRAZ, THIAGO VASCONCELOS; DOS SANTOS, PAMYLA LAYENE; JANEGITZ, BRUNO CAMPOS; BONACIN, JULIANO ALVES. Comparison of activation processes for 3D printed PLA-graphene electrodes: electrochemical properties and application for sensing of dopamine. ANALYST, v. 145, n. 4, p. 1207-1218, FEB 21 2020. Web of Science Citations: 0.

PRADEL-FILHO, LAURO A.; ANDREOTTI, ISABELA A. A.; CARVALHO, JEFFERSON H. S.; ARAUJO, DIELE A. G.; ORZARI, LUIZ O.; GATTI, ALEXANDRE; TAKEUCHI, REGINA M.; SANTOS, ANDRE L.; JANEGITZ, BRUNO C. Glass varnish-based carbon conductive ink: A new way to produce disposable electrochemical sensors. SENSORS AND ACTUATORS B-CHEMICAL, v. 305, FEB 15 2020. Web of Science Citations: 0.

CAMARGO, JESSICA R.; ANDREOTTI, ISABELLA A. A.; KALINKE, CRISTIANE; HENRIQUE, JULIA M.; BONACIN, JULIANO A.; JANEGITZ, BRUNO C. Waterproof paper as a new substrate to construct a disposable sensor for the, electrochemical determination of paracetamol and melatonin. Talanta, v. 208, FEB 1 2020. Web of Science Citations: 0.

AZEVEDO, PATRICIA; BUTOLO, NICOLE PAVAN; DE ALENCAR, LUCIANO DELMONDES; SOARES-LIMA, HELLEN MARIA; SALES, VICTOR RIBEIRO; MALASPINA, OSMAR; FERREIRA NOCELLI, ROBERTA CORNELIO. Standardization of in vitro nervous tissue culture for honeybee: A high specificity toxicological approach. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, v. 189, FEB 2020. Web of Science Citations: 0.

VIDAL RAMOS, MATHEUS MESQUITA; CARVALHO, JEFFERSON H. S.; DE OLIVEIRA, PAULO ROBERTO; JANEGITZ, BRUNO CAMPOS. Determination of serotonin by using a thin film containing graphite, nanodiamonds and gold nanoparticles anchored in casein. MEASUREMENT, v. 149, JAN 2020. Web of Science Citations: 0.

VINICIUS A. O. P. DA SILVA; VINICIUS A. P. TARTARE; CRISTIANE KALINKE; PAULO ROBERTO DE OLIVEIRA; DANIEL CARDOSO DE SOUZA; JULIANO A. BONACIN; BRUNO C. JANEGITZ. CONSTRUÇÃO DE UM SUPORTE AJUSTÁVEL LAB-MADE IMPRESSO EM 3D PARA MEDIÇÃO DE ÂNGULO DE CONTATO. Química Nova, v. 43, n. 9, p. 1312-1319, Set. 2020.

GRELLA, TATIANE CAROLINE; SOARES-LIMA, HELLEN MARIA; MALASPINA, OSMAR; FERREIRA NOCELLI, ROBERTA CORNELIO. Semi-quantitative analysis of morphological changes in bee tissues: A toxicological approach. Chemosphere, v. 236, DEC 2019. Web of Science Citations: 2.

ZAMBIANCO, NAIARA ALANA; SILVA, TIAGO ALMEIDA; ZANIN, HUDSON; FATIBELLO-FILHO, ORLANDO; JANEGITZ, BRUNO CAMPOS. Novel electrochemical sensor based on nanodiamonds and manioc starch for detection of diquat in environmental samples. DIAMOND AND RELATED MATERIALS, v. 98, OCT 2019. Web of Science Citations: 0.

TADEI, RAFAELA; DOMINGUES, CAIO E. C.; MALAQUIAS, JOSE BRUNO; CAMILO, ERASNILSON VIEIRA; MALASPINA, OSMAR; SILVA-ZACARIN, ELAINE C. M. Late effect of larval co-exposure to the insecticide clothianidin and fungicide pyraclostrobin in Africanized Apis mellifera. SCIENTIFIC REPORTS, v. 9, MAR 1 2019. Web of Science Citations: 4.