Lipemia is among the main causes of rejection of samples in veterinary clinical laboratories, interfering with biochemical determinations obtained by spectrophotometry. Recently, our research group has detected that physiological postprandial lipemia causes misinterpretation in various routine canine biochemical determinations. However, little is known about the effect of lipemia on canine oxidative stress parameters, markers that have been widely used in research projects. Considering that lipemia can not always be avoided in the veterinary routine, the present study may guide acceptance or rejection criteria of lipemic samples for determinations of oxidative stress markers in dogs, especially in physiological postprandial conditions. The objective of this study was to evaluate the effect of postprandial lipemia on canine oxidative stress determinations, comparing them to changes caused by simulated lipemia in vitro with commercial lipid solution at levels similar to those obtained in vivo. In the study, 15 healthy dogs will be selected. In the in vivo assay, postprandial lipemia will be induced and compared to two non-lipemic moments: one day before and one day after the induction of postprandial lipemia, with samples always being collected at the same time of day. For the in vitro assay, non-lipemic samples of these animals after a 12 h fasting will be added commercial lipid solution simulating triglyceride concentrations similar to the minimum, medium and maximum values obtained in the in vivo study. The biochemical analysis will be carried out using an automated spectrophotometer using previously published methodology, and the total antioxidant capacity will be determined by three methods (antioxidant capacity equivalent to trolox, plasma ferric reduction capacity and reducible cupric antioxidant capacity), total oxidant capacity, lipid peroxidation by thiobarbituric acid reactive substances, in addition to the antioxidants uric acid, albumin and bilirubins determined by commercial reagents. The variables will be tested for normality and repeated measures ANOVA with Dunnet's post-test or Friedman with Dunn's post-test will be used to verify differences between groups. (AU)
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MINUCCI BONATTO, NATALIA CAMILA;
DE OLIVEIRA, PAULA LIMA;
MANCEBO, ALINE MARTINS;
COSTA, LETICIA RAMOS;
MELO BOSCULO, MARIA RAQUEL;
BOSCO, ANELISE MARIA;
CIARLINI, PAULO CESAR;
FLORIANO, BEATRIZ PEREZ;
DE BARROS, LUIZ DANIEL;
MARTINS DE ALMEIDA, BRENO FERNANDO.
Postprandial lipemia causes oxidative stress in dogs.
Research in Veterinary Science,
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