Plasticity at the myotendinous junction of adult rats subjected to stretching, associated with hypertrophy and swimming training: morphological, molecular and functional analyzes, with new perspectives about sarcomerogenesis

• Grant number: 17/12525-1
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2019
• End date: January 31, 2021
• Field of knowledge: Biological Sciences - Morphology - Anatomy
• Principal Investigator: Adriano Polican Ciena
• Grantee: Adriano Polican Ciena
• Host Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil

Abstract

The myotendinous junction (MTJ) is the main anatomical area of the locomotor apparatus, which comprises the region of interface between muscles and tendons. The main function of MTJ is to transmit the forces generated in muscle contractions by the contractile proteins present in myofibrils to the connective tissue proteins of the tendon during movement. Stretching is a physical activity that, through the longitudinal passive tensional force, causes a mechanical stimulus that determines muscular hypertrophy. Resistance training is recognized both in scientific and clinical practice, due to its health benefits, functional capacity and increase in quality of life. Aerobic exercise has the capacity to promote morphological changes in the MTJ and its applicability in specific pathological situations with a preventive and rehabilitation character. The present study will analyze the possible alterations of the morphological, molecular and functional characteristics in the myotendinous junction of the tibialis anterior and soleus of adult Wistar rats that will be submitted to static stretching previously and associated to different protocols: muscular hypertrophy training and swimming training. Ninety adult Wistar rats, 90 days aged, allocated in 6 different groups will be used: Sedentary Group; Stretched Group; Stretched / hypertrophy Trained Group; Stretched / Trained Swimming Group; Hypertrophy Training Group; Group Trained Swimming. These samples will be processed for morphological and molecular analyzes for the use of light microscopy, immunofluorescence, Western blot assays, transmission electron microscopy, PCR - Real Time, and functional analysis. The results of the present study may contribute to elucidate the possible alterations in the MTJ morphology of Wistar rats, with future purpose of actions in the prevention of injuries and in applications of new rehabilitation protocols. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

JACOB, CAROLINA DOS S.; BARBOSA, GABRIELA K.; RODRIGUES, MARIANA P.; PIMENTEL NETO, JURANDYR; ROCHA-BRAGA, LARA C.; DE OLIVEIRA, CAMILLA G.; CHACUR, MARUCIA; CIENA, ADRIANO P.. Ultrastructural and Molecular Development of the Myotendinous Junction Triggered by Stretching Prior to Resistance Exercise. Microscopy and Microanalysis, v. 28, n. 2, p. 6-pg., 2022-03-08. (17/12525-1)

JACOB, CAROLINA DOS SANTOS; ROCHA, LARA CAETANO; PIMENTEL NETO, JURANDYR; WATANABE, II-SEI; CIENA, ADRIANO POLICAN. Effects of physical training on sarcomere lengths and muscle-tendon interface of the cervical region in an experimental model of menopause. EUROPEAN JOURNAL OF HISTOCHEMISTRY, v. 63, n. 3, p. 131-135, 2019. (17/12525-1, 17/21764-0)

BARBOSA, GABRIELA K.; JACOB, CAROLINA DOS S.; RODRIGUES, MARIANA P.; ROCHA, LARA C.; PIMENTEL NETO, JURANDYR; CIENA, ADRIANO P.. Morphological Changes in the Motor Endplate and in the Belly Muscle Induced by Previous Static Stretching to the Climbing Protocol. Microscopy and Microanalysis, v. 27, n. 5, p. 1183-1191, OCT 2021. (17/12525-1)

NETO, JURANDYR PIMENTEL; ROCHA, LARA CAETANO; JACOB, CAROLINA DOS SANTOS; BARBOSA, GABRIELA KLEIN; CIENA, ADRIANO POLICAN. ostsynaptic cleft density changes with combined exercise protocols in an experimental model of muscular hypertroph. EUROPEAN JOURNAL OF HISTOCHEMISTRY, v. 65, n. 1, SI, 2021. (17/12525-1, 17/21977-3)

NETO, JURANDYR PIMENTEL; ROCHA, LARA CAETANO; BARBOSA, GABRIELA KLEIN; JACOB, CAROLINA DOS SANTOS; NETO, WALTER KRAUSE; WATANABE, II-SEI; CIENA, ADRIANO POLICAN. Myotendinous junction adaptations to ladder-based resistance training: identification of a new telocyte niche. SCIENTIFIC REPORTS, v. 10, n. 1, AUG 24 2020. (17/12525-1, 17/21977-3, 18/09199-8)

ROCHA, LARA CAETANO; BARBOSA, GABRIELA KLEIN; PIMENTEL NETO, JURANDYR; JACOB, CAROLINA DOS SANTOS; KNUDSEN, ANDREAS B.; WATANABE, II-SEI; CIENA, ADRIANO POLICAN. Aquatic Training after Joint Immobilization in Rats Promotes Adaptations in Myotendinous Junctions. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 22, n. 13, JUL 2021. (18/09199-8, 17/12525-1)

ROCHA, LARA CAETANO; JACOB, CAROLINA DOS SANTOS; BARBOSA, GABRIELA KLEIN; PIMENTEL NETO, JURANDYR; KRAUSE NETO, WALTER; GAMA, ELIANE FLORENCIO; CIENA, ADRIANO POLICAN. Remodeling of the skeletal muscle and postsynaptic component after short-term joint immobilization and aquatic training. Histochemistry and Cell Biology, v. 154, n. 6, AUG 2020. (17/12525-1)

REGINATO, GABRIELA DE SOUZA; BARBOSA, GABRIELA KLEIN; FERREIRA, AMANDA OLIVOTTI; VASCONCELOS, BRUNO GOMES; GRASSI RICI, ROSE ELI; WATANABE, II-SEI; CIENA, ADRIANO POLICAN. Morphological and ultrastructural characteristics of the tongue of wild boar. EUROPEAN JOURNAL OF HISTOCHEMISTRY, v. 64, n. 2, p. 156-163, 2020. (17/12525-1)