COMPARATIVE ANALYSIS OF VIRULENCE CHARACTERISTICS OF DIARRHEIOGENIC Escherichia coli IN E. coli STRAINS ISOLATED FROM PATIENTS WITH DIFFERENT TYPES OF URINARY INFECTION

• Grant number: 18/17353-7
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2019
• End date: January 31, 2021
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Tânia Aparecida Tardelli Gomes do Amaral
• Grantee: Tânia Aparecida Tardelli Gomes do Amaral
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated researchers: Ana Carolina de Mello Santos Gheller ; Ivan Nicolau Falsetti ; Mônica Aparecida Midolli Vieira ; Rosa Maria Silva

Abstract

Some adapted clones of Escherichia coli carry genes that encode properties that allow them to colonize different host sites, favoring the establishment of intestinal or extraintestinal infections. Intestinal infections are caused by different diarrheogenic E. coli (DEC) pathotypes, while the extraintestinal infections are caused by strains of extraintestinal pathogenic E. coli (ExPEC). ExPEC strains isolated from urinary tract infection (UTI) (cystitis and/or pyelonephritis) are also called uropathogenic E. coli (UPEC). Recent reports have shown that certain virulence genes, located on Pathogenicity Islands or plasmids, can be transferred from DEC strains to ExPEC or vice versa, leading to the formation of hybrid strains, which have the potential to cause more severe extraintestinal infections or even intestinal infections. However, we are unaware of studies that evaluated the potential of colonization of the hybrid UPECs and determined their genotypic and phenotypic diversity. In this study, the occurrence of hybrid UPEC strains will be evaluated in patients with cystitis, pyelonephritis and asymptomatic urinary infections. Hybrid and non-hybrid strains will be compared for serotypes, phylogenetic and clonal groups, presence of plasmids, biofilm production and adherence capacity in vitro. As part of UTIs occurs due to colonization of the urethra by strains from the feces, hybrid UPEC strains will also be screened in the feces of the patients studied and evaluated for a potential relationship with occurrence of diarrhea. A comparative analysis of the genome of selected hybrid strains will also be performed. The knowledge generated in this study will contribute to the elucidation of the pathogenesis of hybrid UPECs in UTIs and their potential specificity regarding colonization sites and the capacity to generate intestinal infections in the same host, contributing to a more adequate management of these diseases of extreme global relevance. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE MELLO SANTOS, ANA CAROLINA; FUGA, BRUNA; ESPOSITO, FERNANDA; CARDOSO, BRENDA; SANTOS, FERNANDA FERNANDES; VALIATTI, TIAGO BARCELOS; SANTOS-NETO, JOSE FRANCISCO; GALES, ANA CRISTINA; LINCOPAN, NILTON; SILVA, ROSA MARIA; et al. Unveiling the Virulent Genotype and Unusual Biochemical Behavior of Escherichia coli ST59. Applied and Environmental Microbiology, v. 87, n. 16, AUG 2021. (19/15578-4, 09/00402-6, 18/17353-7)

VALIATTI, TIAGO B.; SANTOS, FERNANDA F.; SANTOS, ANA C. M.; NASCIMENTO, JULLIA A. S.; SILVA, ROSA M.; CARVALHO, ENEAS; SINIGAGLIA, RITA; GOMES, TANIA A. T.. Genetic and Virulence Characteristics of a Hybrid Atypical Enteropathogenic and UropathogenicEscherichia coli(aEPEC/UPEC) Strain. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 10, SEP 29 2020. (18/17353-7, 17/21947-7)

SANTOS, ANA CAROLINA M.; SILVA, ROSA M.; VALIATTI, TIAGO B.; SANTOS, FERNANDA E.; SANTOS-NETO, JOSE E.; CAYO, RODRIGO; STRELING, ANA P.; NODARI, CAROLINA S.; GALES, ANA C.; NISHIYAMA-JR, MILTON Y.; et al. Virulence Potential of a Multidrug-ResistantEscherichia coliStrain Belonging to the Emerging Clonal Group ST101-B1 Isolated from Bloodstream Infection. MICROORGANISMS, v. 8, n. 6, JUN 2020. (16/01656-5, 17/21947-7, 09/00402-6, 18/17353-7)

NASCIMENTO, JULLIA A. S.; SANTOS, FERNANDA F.; VALIATTI, TIAGO B.; SANTOS-NETO, JOSE F.; SANTOS, ANA CAROLINA M.; CAYO, RODRIGO; GALES, ANA C.; GOMES, TANIA A. T.. Frequency and Diversity of Hybrid Escherichia coli Strains Isolated from Urinary Tract Infections. MICROORGANISMS, v. 9, n. 4, APR 2021. (17/21947-7, 18/17353-7, 19/14553-8, 19/21685-8)

SANTOS, ANA CAROLINA M.; SANTOS-NETO, JOSE F.; TROVAO, LIANA O.; ROMANO, RICARDO F. T.; SILVA, ROSA MARIA; GOMES, TANIA A. T.. Characterization of unconventional pathogenic Escherichia coli isolated from bloodstream infection: virulence beyond the opportunism. Brazilian Journal of Microbiology, v. N/A, p. 14-pg., 2022-12-08. (19/21685-8, 17/14821-7, 18/17353-7)

VALIATTI, TIAGO BARCELOS; SANTOS, FERNANDA FERNANDES; DE MELLO SANTOS, ANA CAROLINA; SILVA, ROSA MARIA; CARVALHO, ENEAS; TARDELLI GOMES, TANIA APARECIDA. Draft Whole-Genome Sequence of a Uropathogenic Escherichia coli Strain Carrying the eae Gene. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 8, n. 43, OCT 2019. (17/21947-7, 18/17353-7)

NASCIMENTO, JULLIA A. S.; SANTOS, FERNANDA F.; SANTOS-NETO, JOSE F.; TROVAO, LIANA O.; VALIATTI, TIAGO B.; PINAFFI, ISABEL C.; VIEIRA, MONICA A. M.; SILVA, ROSA M.; FALSETTI, IVAN N.; SANTOS, ANA C. M.; et al. Molecular Epidemiology and Presence of Hybrid Pathogenic Escherichia coli among Isolates from Community-Acquired Urinary Tract Infection. MICROORGANISMS, v. 10, n. 2, p. 17-pg., 2022-02-01. (19/14553-8, 19/21685-8, 17/14821-7, 18/17353-7)

TANABE, RODRIGO H. S.; DIAS, REGIANE C. B.; ORSI, HENRIQUE; DE LIRA, DAIANY R. P.; VIEIRA, MELISSA A.; DOS SANTOS, LUIS F.; FERREIRA, ADRIANO M.; RALL, VERA L. M.; MONDELLI, ALESSANDRO L.; GOMES, TANIA A. T.; et al. Characterization of Uropathogenic Escherichia coli Reveals Hybrid Isolates of Uropathogenic and Diarrheagenic (UPEC/DEC) E. coli. MICROORGANISMS, v. 10, n. 3, p. 15-pg., 2022-03-01. (15/26207-6, 18/17353-7)