Gene expression and protein synthesis analysis of dental pulp cells from deciduous teeth after the use of Low Intensity Laser.

• Grant number: 18/20316-6
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2019
• End date: January 31, 2021
• Field of knowledge: Health Sciences - Dentistry - Pediatric Dentistry
• Principal Investigator: Thais Marchini de Oliveira Valarelli
• Grantee: Thais Marchini de Oliveira Valarelli
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

This study aimed to evaluate the effect of Low Intensity Laser on cell viability, cell proliferation, gene expression and protein synthesis of cells derived from the pulp of human deciduous teeth after the variation of the parameters of photobiomodulation. Cells samples will be obtained and grown in specific culture medium with Fetal Bovine Serum (FBS). After the fourth passage, the sample will be irradiated with low intensity laser according to the photobiomodulation protocol by varying the power and the irradiation time obtaining different energy densities (1.2 to 6.2 J / cm2). The control groups of each experiment will be treated under identical conditions, except that the laser device will be kept off during the experiment. Viability analysis (MTT assay) and cell proliferation (crystal violet assay) will be performed at 6, 12 and 24 hours after irradiation. The gene expression of angiogenic growth factors (VEGF-A, VEGF-D, VEGFR1, VEGFR2, FGF2, PLGF, PDGF, PECAM-1 and BMP-9) will be quantified in the cell lysate by RT-PCR in 6, 12, and 24 hours after irradiation. Protein synthesis of the same growth factors will be quantified in the supernatant and cell lysate by ELISA multiplex assay at 6, 12 and 24 hours post irradiation. The values in triplicate will be analyzed of each experiment. The means and standard deviation will be tested for normality test. If the data present normal distribution, intragroup and intergroup comparison will be performed by ANOVA at two criteria, followed by the Tukey test. If the data do not present normal distribution, the intragroup comparison will be performed by the Friedman Test and the intergroup comparison will be performed by the Kruskal-Wallis test. The significance level of 5% will be adopted for differences to be considered statistically significant. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

OLIVEIRA PRADO BERGAMO, MARIEL TAVARES; RIBEIRO VITOR, LUCIANA LOURENCO; LOPES, NATHALIA MARTINS; LOURENCO NETO, NATALINO; DIONISIO, THIAGO JOSE; OLIVEIRA, RODRIGO CARDOSO; RODINI, CAMILA OLIVEIRA; SANTOS, CARLOS FERREIRA; ANDRADE MOREIRA MACHADO, MARIA APARECIDA; OLIVEIRA, THAIS MARCHINI. Angiogenic protein synthesis after photobiomodulation therapy on SHED: a preliminary study. Lasers in Medical Science, v. 35, n. 9, FEB 2020. (17/11396-3, 18/20316-6)

RIBEIRO VITOR, LUCIANA LOURENCO; OLIVEIRA PRADO, MARIEL TAVARES; NETO, NATALINO LOURENCO; OLIVEIRA, RODRIGO CARDOSO; SAKAI, VIVIEN THIEMY; SANTOS, CARLOS FERREIRA; DIONISIO, THIAGO JOSE; RIOS, DANIELA; CRUVINEL, THIAGO; ANDRADE MOREIRA MACHADO, MARIA APARECIDA; et al. Does photobiomodulation change the synthesis and secretion of angiogenic proteins by different pulp cell lineages?. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 203, JAN 2020. (18/20316-6, 13/18886-5)

BERGAMO, MARIEL TAVARES; VITOR, LUCIANA LOURENCO RIBEIRO; DIONISIO, THIAGO JOSE; MARQUES, NADIA CAROLINA TEIXEIRA; OLIVEIRA, RODRIGO CARDOSO; AMBROSIO, ELOA CRISTINA PASSUCCI; SAKAI, VIVIEN THIEMY; SANTOS, CARLOS FERREIRA; LOURENCO NETO, NATALINO; MACHADO, MARIA APARECIDA ANDRADE MOREIRA; et al. Could the photobiomodulation therapy induce angiogenic growth factors expression from dental pulp cells?. Lasers in Medical Science, APR 2021. (18/20316-6, 17/11396-3)

RIBEIRO VITOR, LUCIANA LOURENCO; OLIVEIRA PRADO BERGAMO, MARIEL TAVARES; LOURENCO-NETO, NATALINO; SAKAI, VIVIEN THIEMY; OLIVEIRA, RODRIGO CARDOSO; CRUVINEL, THIAGO; RIOS, DANIELA; GARLET, GUSTAVO POMPERMAIER; SANTOS, CARLOS FERREIRA; ANDRADE MOREIRA MACHADO, MARIA APARECIDA; et al. Photobiomodulation effect on angiogenic proteins produced and released by dental pulp cells. CLINICAL ORAL INVESTIGATIONS, v. 24, n. 12, APR 2020. (18/20316-6)

BERGAMO, MARIEL TAVARES; VITOR, LUCIANA LOURENCO RIBEIRO; DIONISIO, THIAGO JOSE; MARQUES, NADIA CAROLINA TEIXEIRA; OLIVEIRA, RODRIGO CARDOSO; AMBROSIO, ELOA CRISTINA PASSUCCI; SAKAI, VIVIEN THIEMY; SANTOS, CARLOS FERREIRA; LOURENCO NETO, NATALINO; MACHADO, MARIA APARECIDA ANDRADE MOREIRA; et al. Could the photobiomodulation therapy induce angiogenic growth factors expression from dental pulp cells?. Lasers in Medical Science, v. 36, n. 8, p. 1751-1758, OCT 2021. (17/11396-3, 18/20316-6)