Nanostructured silica as a protective vehicle for vaccines and biomolecules

• Grant number: 17/17844-8
• Support Opportunities: Research Projects - Thematic Grants
• Duration: February 01, 2019 - January 31, 2025
• Field of knowledge: Biological Sciences - Immunology - Applied Immunology
• Principal Investigator: Osvaldo Augusto Brazil Esteves Sant'Anna
• Grantee: Osvaldo Augusto Brazil Esteves Sant'Anna
• Host Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
• Pesquisadores principais: Marcia Carvalho de Abreu Fantini ; Tereza da Silva Martins
• Associated researchers: Andrea Borrego ; Aryene Góes Trezena ; Cristiano Luis Pinto de Oliveira ; Daniela Gomes da Silva ; Denise Vilarinho Tambourgi ; Francisco Mariano Neto ; Gisele Picolo ; Helio Jose Montassier ; Heloisa Nunes Bordallo ; Jose Luiz de Souza Lopes ; Luís Carlos Cides da Silva ; Luís Guilherme de Oliveira ; Marcelo de Franco ; Marcos Tadeu Dazeredo Orlando ; Matheus Carlos Romeiro Miranda ; Milene Tino De Franco ; Newton Andréo Filho ; Orlando Garcia Ribeiro Filho ; Patricia Antonia Estima Abreu de Aniz ; Patricia Santos Lopes ; Paulo Lee Ho ; Pedro Leonidas Oseliero Filho ; Solange Massa ; Viviane Fongaro Botosso ; Wafa Hanna Koury Cabrera
• Associated grant(s): 19/08582-5 - Multi-User Equipment approved in grant 17/17844-8: thermal analyzer - TG/DTA model sta 2500 TG/DTA room temperature to 1100 °c, AP.EMU; 19/07007-7 - Multi-User Equipment approved in grant 17/17844-8: SAXS detector system upgrade with sample holder and window, AP.EMU
• Associated scholarship(s): 24/06183-4 - Synthesis and characterization of new mesoporous silica, BP.TT; 22/08360-5 - Study of the influence of the morphological properties of SBA-15 on the efficacy of molecules with therapeutic potential and vaccines, BP.PD; 21/13055-4 - Structure swelling agents in the synthesis of mesoporous silica, BP.IC; + associated scholarships 20/04063-0 - Synthesis and characterization of silicas with cubic mesoporous structure: FDU-1 and SBA-16, BP.IC; 19/19567-7 - Structural studies of proteins incorporated in ordered mesoporous silica, BP.MS; 19/12301-1 - Synthesis of ordered mesoporous silica with different structures and morphologies, BP.PD - associated scholarships

Abstract

Research work on vaccines, specially administer by oral route, has been successfully developed through cooperation among Butantan Institute and Institute of Physics of the University of São Paulo, and a national pharmaceutical industry, the Cristália Laboratory. This partnership is a project of public-private responsibility, due to the characteristics of its open joint research processes and the assignment of a patent for national use, without burden to Brazil. Pioneer previous studies gave rise to an innovative scientific and technological approach, which needs to be expanded for use in poly-vaccines and new biomolecules. These studies are based on the use of nanostructured silica as adjuvant /vehicle to be made available to the society, aiming higher effectiveness to the existing products on the market and lower cost. The present multidisciplinary project purposes to synthesize, characterize and determine the efficacy of distinct nanostructured silicas with different pore structures and morphologies for the development of Diphtheria, Tetanus (DT) and Hepatitis B vaccines for oral and / or parenteral administration and also to decrease the toxicity of venoms and toxins, as already verified in preliminary studies. Vaccine product analyzes contemplate the possibility of decreased protein concentrations, reduction in the number of doses, and reevaluations over dose intervals in correlation with the induction of effective immunological memory. Preliminary studies indicate that SBA-15 makes it possible to reduce the toxicity of bacterial exotoxins and here this characteristic will be studied including venoms and toxins from snakes and spiders. In addition, silicas will be used as a protective vehicle in order to favor the effect of Crotoxin (CTX) on the control of chronic pain, its interference on the immune and inflammatory responses induced by Experimental Autoimmune Encephalomyelitis (EAE), and neuropathy models as Peripheral nerve injury (PSNL). (AU)

Scientific publications

(16)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MECHLER-DREIBI, MARINA L.; ALMEIDA, HENRIQUE M. S.; SONALIO, KARINA; MARTINES, MARIELA A. C.; PETRI, FERNANDO A. M.; ZAMBOTTI, BEATRIZ B.; FERREIRA, MARCELA M.; STORINO, GABRIEL Y.; MARTINS, TEREZA S.; MONTASSIER, HELIO J.; et al. Oral vaccination of piglets against Mycoplasma hyopneumoniae using silica SBA-15 as an adjuvant effectively reduced consolidation lung lesions at slaughter. SCIENTIFIC REPORTS, v. 11, n. 1, NOV 17 2021. (18/12742-5, 17/17844-8, 19/19710-4)

DONADON, LARISSA GABRIELLA FUSCO; SALATA, GIOVANNA CASSONE; GONCALVES, THALITA PEDRALINO; MATOS, LISA DE CARVALHO; EVANGELISTA, MARIA CLARA PAIVA; DA SILVA, NICOLE SAMPAIO; MARTINS, TEREZA SILVA; MACHADO-NETO, JOAO AGOSTINHO; LOPES, LUCIANA BIAGINI; GARCIA, MARIA TERESA JUNQUEIRA. Monoolein-based nanodispersions for cutaneous co-delivery of methylene blue and metformin: Thermal and structural characterization and effects on the cutaneous barrier, skin penetration and cytotoxicity. International Journal of Pharmaceutics, v. 633, p. 18-pg., 2023-02-01. (17/18239-0, 18/13877-1, 17/17844-8, 19/08582-5, 19/26048-6)

ZAMBELLI, VANESSA O.; HOSCH, NAT PRIME ALIA GABRIELE; FAROM, SARAH; ZYCHAR, BIANCA C.; SPADACCI-MORENA, DIVA D.; CARVALHO, LUCIANA VIEIRA; CURI, RUI; LEPSCH, LUCILIA B.; SCAVONE, CRISTOFORO; SANT'ANNA, OSVALDO AUGUSTO; et al. Formyl peptide receptors are involved in CTX-induced impairment of lymphocyte functions. Toxicon, v. 222, p. 10-pg., 2023-01-15. (08/57898-0, 17/17844-8)

DO PRADO, ALICE HADDAD; DUARTE, JONATAS LOBATO; DI FILIPPO, LEONARDO DELELLO; VICTORELLI, FRANCESCA DAMIANI; DE ABREU FANTINI, MARCIA CARVALHO; PECCININI, ROSANGELA GONACALVES; CHORILLI, MARLUS. Bioadhesive liquid crystal systems for octyl methoxycinnamate skin delivery. JOURNAL OF MOLECULAR LIQUIDS, v. 345, JAN 1 2022. (16/24531-3, 14/22426-2, 17/17844-8)

LOSITO, DANILO W.; LOPES, PATRICIA S.; UEOKA, ANDREZA R.; FANTINI, MARCIA C. A.; OSELIERO FILHO, PEDRO LEONIDAS; ANDREO-FILHO, NEWTON; MARTINS, TEREZA S.. Biocomposites based on SBA-15 and papain: Characterization, enzymatic activity and cytotoxicity evaluation. Microporous and Mesoporous Materials, v. 325, OCT 2021. (19/12301-1, 17/26618-1, 17/17844-8, 16/22916-5, 20/13204-7, 19/08582-5)

RASMUSSEN, MARTIN KJAERULF; BORDALLO, HELOISA N.; BORDENALLI, MARCELA APARECIDA; AKAMATSU, MILENA APETITO; TREZENA, ARYENE GOES; TINO-DE-FRANCO, MILENE; SANT'ANNA, OSVALDO A.; MARTINS, TEREZA DA SILVA; DE SOUZA LOPES, JOSE LUIZ; DE ABREU FANTINI, MARCIA CARVALHO; et al. Assessing the efficiency of SBA-15 as a nanocarrier for diphtheria anatoxin. Microporous and Mesoporous Materials, v. 312, JAN 2021. (17/17844-8, 18/19546-7)

TAKAMORI, DANTE YUGO; ALVES, RAFAELA TEIXEIRA; GOUVEIA-NETO, ARTUR DA SILVA; BUENO, LUCIANO AVALLONE; MANZANI, DANILO; MOLINA, CELSO; MARTINS, TEREZA SILVA. Synthesis and luminescence investigation of SBA-15/NaYF4:Yb/Er composites. Journal of Sol-Gel Science and Technology, v. 97, n. 1, p. 167-177, JAN 2021. (14/50869-6, 17/17844-8)

TREZENA, ARYENE GOES; OSELIERO FILHO, PEDRO LEONIDAS; DA SILVA, LUIS CARLOS CIDES; OLIVEIRA, CRISTIANO LUIS PINTO; LOPES, JOSE LUIZ DE SOUZA; ANTONIO, NAYARA DA SILVA; DETTMANN, VIKTOR FONSECA BARBOSA; AKAMATSU, MILENA APETITO; MARTINS, TEREZA DA SILVA; RIBEIRO, ORLANDO GARCIA; et al. Adjuvant effect of mesoporous silica SBA-15 on anti-diphtheria and anti-tetanus humoral immune response. BIOLOGICALS, v. 80, p. 9-pg., 2022-10-01. (17/17844-8, 20/13204-7, 19/12301-1)

OLIVEIRA, C. L. P.; LOPES, J. L. S.; SANT'ANNA, O. A.; BOTOSSO, V. F.; BORDALLO, H. N.; FANTINI, M. C. A.. The development of new oral vaccines using porous silica. JOURNAL OF PHYSICS-CONDENSED MATTER, v. 34, n. 26, p. 10-pg., 2022-06-29. (17/17844-8, 18/19546-7)

LOSITO, DANILO WAISMANN; DE ARAUJO, DANIELE RIBEIRO; NONATO BEZZON, VINICIUS DANILO; OSELIERO FILHO, PEDRO LEONIDAS; AFFONSO FONSECA, FERNANDO LUIZ; CHAGAS, CAMILA DOS SANTOS; BARBOSA, EMERSON; PINTO OLIVEIRA, CRISTIANO LUIS; DE ABREU FANTINI, MARCIA CARVALHO; FERREIRA, FABIO FURLAN; et al. esoporous Silica-Fe3O4 Nanoparticle Composites as Potential Drug Carrier. ACS APPLIED NANO MATERIALS, v. 4, n. 12, p. 13363-13378, DEC 24 2021. (20/13204-7, 14/50869-6, 17/17844-8, 18/11990-5, 19/12301-1)

SANT'ANNA, MORENA BRAZIL; RIBEIRO LOPES, FLAVIA SOUZA; KIMURA, LOUISE FAGGIONATO; GIARDINI, ALINE CAROLINA; SANT'ANNA, OSVALDO AUGUSTO; PICOLO, GISELE. Crotoxin Conjugated to SBA-15 Nanostructured Mesoporous Silica Induces Long-Last Analgesic Effect in the Neuropathic Pain Model in Mice. TOXINS, v. 11, n. 12, DEC 2019. (15/01254-1, 14/19397-0, 17/17844-8, 08/57898-0)

SANT'ANNA, MORENA BRAZIL; GIARDINI, ALINE C.; RIBEIRO, MARCIO A. C.; LOPES, FLAVIA S. R.; TEIXEIRA, NATHALIA B.; KIMURA, LOUISE F.; BUFALO, MICHELLE C.; RIBEIRO, ORLANDO G.; BORREGO, ANDREA; CABRERA, WAFA H. K.; et al. The Crotoxin:SBA-15 Complex Down-Regulates the Incidence and Intensity of Experimental Autoimmune Encephalomyelitis Through Peripheral and Central Actions. FRONTIERS IN IMMUNOLOGY, v. 11, OCT 28 2020. (17/17844-8, 15/01254-1, 14/19397-0, 13/07467-1)

FERREIRA, GEOVANA C.; SANCHES, THAINARA V. C.; MECHLER-DREIBI, MARINA L.; ALMEIDA, HENRIQUE M. S.; STORINO, GABRIEL Y.; SONALIO, KARINA; PETRI, FERNANDO A. M.; MARTINS, TEREZA S.; DA SILVA, LUIS CARLOS CIDES; MONTASSIER, HELIO J.; et al. Efficacy evaluation of a novel oral silica-based vaccine in inducing mucosal immunity against Mycoplasma hyopneumoniae. Research in Veterinary Science, v. 158, p. 10-pg., 2023-03-31. (20/04707-5, 18/12742-5, 17/17844-8, 19/19710-4, 20/08028-5, 21/11914-0)

SANT'ANNA, M. B.; GIARDINI, A. C.; LOPES, F. S.; KIMURA, L. F.; CHACUR, M.; PAGANO, R. L.; ZAMBELLI, V. O.; SANT'ANNA, O. A.; PICOLO, G.. RATTLESNAKE VENOM COMPONENTS IN THE CONTROL OF EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS: IMMUNOMODULATORY PROPERTIES POTENTIATED BY SILICA SBA-15. Toxicon, v. 182, p. 1-pg., 2020-07-01. (13/07467-1, 17/17844-8)

LOPES, J. L. S.; OLIVEIRA, D. C. A.; UTESCHER, C. L. A.; QUINTILIO, W.; TENORIO, E. C. N.; OLIVEIRA, C. L. P.; FANTINI, M. C. A.; RASMUSSEN, M. K.; BORDALLO, H. N.; SANT'ANNA, O. A.; et al. Antigenic and physicochemical characterization of Hepatitis B surface protein under extreme temperature and pH conditions. Vaccine, v. 37, n. 43, p. 6415-6425, OCT 8 2019. (17/17844-8, 18/19546-7)

DE ABREU FANTINI, MARCIA CARVALHO; PINTO OLIVEIRA, CRISTIANO LUIS; DE SOUZA LOPES, JOSE LUIZ; MARTINS, TEREZA DA SILVA; AKAMATSU, MILENA APETITO; TREZENA, ARYENE GOES; TINO-DE-FRANCO, MILENE; BOTOSSO, VIVIANE FONGARO; BRAZIL ESTEVES SANT'ANNA, OSVALDO AUGUSTO; KARDJILOV, NIKOLAY; et al. sing crystallography tools to improve vaccine formulation. IUCRJ, v. 9, n. 1, p. 11+, JAN 2022. (17/17844-8, 18/19546-7)