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Nanostructured silica as a protective vehicle for vaccines and biomolecules

Grant number: 17/17844-8
Support type:Research Projects - Thematic Grants
Duration: February 01, 2019 - January 31, 2024
Field of knowledge:Biological Sciences - Immunology - Applied Immunology
Principal Investigator:Osvaldo Augusto Brazil Esteves Sant'Anna
Grantee:Osvaldo Augusto Brazil Esteves Sant'Anna
Home Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
Co-Principal Investigators:Marcia Carvalho de Abreu Fantini ; Tereza da Silva Martins
Assoc. researchers:Andrea Borrego ; Aryene Góes Trezena ; Cristiano Luis Pinto de Oliveira ; Denise Vilarinho Tambourgi ; Francisco Mariano Neto ; Gisele Picolo ; Heloisa Nunes Bordallo ; Jose Luiz de Souza Lopes ; Luís Carlos Cides da Silva ; Marcos Tadeu Dazeredo Orlando ; Milene Tino de Franco ; Orlando Garcia Ribeiro Filho ; Viviane Fongaro Botosso
Associated grant(s):19/08582-5 - Multi-User Equipment approved in grant 17/17844-8: thermal analyzer - TG/DTA model sta 2500 TG/DTA room temperature to 1100 °c, AP.EMU
19/07007-7 - Multi-User Equipment approved in grant 17/17844-8: SAXS detector system upgrade with sample holder and window, AP.EMU
Associated scholarship(s):20/04063-0 - Synthesis and characterization of silicas with cubic mesoporous structure: FDU-1 and SBA-16, BP.IC
19/19567-7 - Structural studies of proteins incorporated in ordered mesoporous silica, BP.MS
19/12301-1 - Synthesis of ordered mesoporous silica with different structures ánd morphologies, BP.PD


Research work on vaccines, specially administer by oral route, has been successfully developed through cooperation among Butantan Institute and Institute of Physics of the University of São Paulo, and a national pharmaceutical industry, the Cristália Laboratory. This partnership is a project of public-private responsibility, due to the characteristics of its open joint research processes and the assignment of a patent for national use, without burden to Brazil. Pioneer previous studies gave rise to an innovative scientific and technological approach, which needs to be expanded for use in poly-vaccines and new biomolecules. These studies are based on the use of nanostructured silica as adjuvant /vehicle to be made available to the society, aiming higher effectiveness to the existing products on the market and lower cost. The present multidisciplinary project purposes to synthesize, characterize and determine the efficacy of distinct nanostructured silicas with different pore structures and morphologies for the development of Diphtheria, Tetanus (DT) and Hepatitis B vaccines for oral and / or parenteral administration and also to decrease the toxicity of venoms and toxins, as already verified in preliminary studies. Vaccine product analyzes contemplate the possibility of decreased protein concentrations, reduction in the number of doses, and reevaluations over dose intervals in correlation with the induction of effective immunological memory. Preliminary studies indicate that SBA-15 makes it possible to reduce the toxicity of bacterial exotoxins and here this characteristic will be studied including venoms and toxins from snakes and spiders. In addition, silicas will be used as a protective vehicle in order to favor the effect of Crotoxin (CTX) on the control of chronic pain, its interference on the immune and inflammatory responses induced by Experimental Autoimmune Encephalomyelitis (EAE), and neuropathy models as Peripheral nerve injury (PSNL). (AU)

Matéria(s) publicada(s) na Agência FAPESP sobre o auxílio:
Novel formulation permits use of toxin extracted from rattlesnake venom to treat chronic pain 
Nanotechnology delivers hepatitis B vaccine 

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SANT'ANNA, MORENA BRAZIL; RIBEIRO LOPES, FLAVIA SOUZA; KIMURA, LOUISE FAGGIONATO; GIARDINI, ALINE CAROLINA; SANT'ANNA, OSVALDO AUGUSTO; PICOLO, GISELE. Crotoxin Conjugated to SBA-15 Nanostructured Mesoporous Silica Induces Long-Last Analgesic Effect in the Neuropathic Pain Model in Mice. TOXINS, v. 11, n. 12 DEC 2019. Web of Science Citations: 1.
LOPES, J. L. S.; OLIVEIRA, D. C. A.; UTESCHER, C. L. A.; QUINTILIO, W.; TENORIO, E. C. N.; OLIVEIRA, C. L. P.; FANTINI, M. C. A.; RASMUSSEN, M. K.; BORDALLO, H. N.; SANT'ANNA, O. A.; BOTOSSO, V. F. Antigenic and physicochemical characterization of Hepatitis B surface protein under extreme temperature and pH conditions. Vaccine, v. 37, n. 43, p. 6415-6425, OCT 8 2019. Web of Science Citations: 0.

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