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Impact of co-exposure to triclosan and to phthalate DEHP on genetic stability and on expression profile of genes related to metabolism of toxicants, to antioxidant system and to DNA repair, in HepG2 cells

Abstract

Triclosan (TCS) is an antimicrobial agent widely used in personal hygiene products (PHP) and diethylhydroxyl (DEHP) is a phthalate group of chemical compounds derived from phthalic acid and is used in medical equipment and plastic products with chlorinated polyvinyls. As a result of their extensive use, these toxicants have been found in the environment and previous studies have demonstrated the relationship between their exposure and various toxic effects. However, there is scarcity in the literature of its toxic effects in mammals and especially in humans. In addition, no studies were carried out to evaluate the co-exposure of these toxicants in laboratory models. Thus, the aim of this work is to evaluate the cytotoxicity, genotoxicity, mutagenicity and expression profile of a panel of genes associated with metal metabolism, as well as genes related to the antioxidant status and DNA repair, in liver cell culture (HepG2) co-exposed to TCS and DEHP. Therefore, the project aims to increase the understanding of the mechanisms of toxicity involved in a co-exposure model, representing a much more real scenario than the evaluation per se of each toxicant. (AU)