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Transcriptoma analysis of the imune system genes through RT-PCR in cultivated macrophages in vitro of tilapia feeded with immunostimulants


The immunology of aquatic organisms is a recent issue of huge global importance, since the intensification of aquatic organisms production have led to the diseases outbreaks and hence the growing interest in the defense mechanisms in order to prevent and treat, avoiding the fish mortality and huge economic losses. Immunostimulants may be a viable alternative to avoid economic losses caused by diseases, among them, ²-glucan is one of the most applied and studied compound in fish. Thus, the present proposal aims to standardize and establish methodology of transcriptome analysis of RT-PCR method, in cultured macrophages, technology that has not been assessed in the Laboratory of Immunology and Molecular Biology, and was not contemplated by JP, in which this proposal is linked. As well as, including the clarification of the possible so-called "trained immunity" (the prolonged innate immunity) in tilapias, and evaluating the effects of ²-glucan on the genes expression of the immune system, and their influence on the intestinal microbiota, in order to determine if the immunomodulatory effects on the organism is due to its direct action on the leukocytes or indirectly on the microbiological populations in the intestine. And to test pre-defined ²-glucan immunomodulation protocols in tilapia. And to evaluate if the immunostimulant has systemic action on the mucosal immunity (skin, gills and intestine). In order to understand the mechanism of action of ²-glucan, and to analyze its immunostimulant potential to the development of new feed protocols and possibly new vaccines. (AU)

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