Chronic kidney disease in dogs: identification of podocyturia and evaluation of renin-angiotensin-aldosterone system components

• Grant number: 19/03651-9
• Support Opportunities: Regular Research Grants
• Start date: May 01, 2019
• End date: September 30, 2021
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Deise Carla Almeida Leite Dellova
• Grantee: Deise Carla Almeida Leite Dellova
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
• Associated researchers: Heidge Fukumasu

Abstract

The normal glomerular function depends on the integrity, differentiation and adhesion of the podocytes to the glomerular basement membrane, both components of the glomerular filtration barrier (GFB). The detachment and loss of podocytes through the urine (podocyturia) occurs naturally, but may increase in disease, being accompanied by proteinuria. In chronic kidney disease (CKD), irreversible degeneration of nephrons occurs, and in the remaining glomeruli, podocytes are subject to mechanical and oxidative stress, in part, by the renin-angiotensin-aldosterone system (RAAS) activation. However, little is known about podocyturia and its relationship with RAAS in dogs with naturally occurring CKD. The objective of this project is to identify podocyturia, through the urinary podocyte-associated mRNA (mRNA for nephrine and podocin) in healthy dogs and with CKD, trying to characterize physiological and CKD-related podocyturia. The plasma renin activity (PRA) and plasma aldosterone concentration ([aldo]p) will be determined in the same dogs to evaluate the RAAS activity and a possible effect of this system on podocyturia. The podocyturia identification can be an important parameter to aid in the diagnosis and differentiation between the stages of CKD in dogs. In addition, the RAAS role on podocyturia and the CKD itself can be explored by the PRA and [aldo]p. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE SOUZA, CAMILLA; COELHO, MARIANA; ANTONELO, DANIEL SILVA; PASSARELLI, DANIELLE; ROCHETTI, ARINA LAZARO; FUKUMASU, HEIDGE; ALMEIDA LEITE-DELLOVA, DEISE CARLA. Nephrin and podocin mRNA detection in urine sediment of dogs with chronic kidney disease: preliminary observations. JOURNAL OF VETERINARY RESEARCH, v. 66, n. 2, p. 8-pg., 2022-04-22. (14/02493-7, 19/03651-9)