A multi-disciplinary approach to the study of amphibian diversification: phase 2

• Grant number: 18/15425-0
• Support type: Research Grants - Young Investigators Grants- Phase 2
• Duration: May 01, 2019 - April 30, 2024
• Field of knowledge: Biological Sciences - Zoology
• Principal researcher: Taran Grant
• Grantee: Taran Grant
• Home Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Assoc. researchers: Almut Kelber ; Carlos Alberto Gonçalves Silva Jared ; Julian Faivovich ; Marta Maria Antoniazzi ; Michele Pierotti ; Ralph Saporito
• Associated research grant: 12/10000-5 - A multi-disciplinary approach to the study of amphibian diversification, AP.JP
• Associated scholarship(s): 21/01015-8 - Technical Training (TT3) Scholarship: Specimen Collection, Preparation, Databasing, and Live Animal Husbandry, BP.TT; 19/15910-9 - Technical training (TT3) scholarship: specimen collection, preparation, databasing, and live animal husbandry, BP.TT; 19/15969-3 - Tissue management, databasing and molecular biology, BP.TT

Abstract

This project is developed along three overlapping and interrelated lines of investigation: amphibian systematics, chemical defense, and ecology and conservation biology. We will set up a cleanroom laboratory to obtain DNA sequences from historical museum specimens and will develop and implement procedures to obtain DNA sequences from a new set of 1041 nuclear DNA markers using next-generation sequencing methods. In addition to general studies of phenomic characters for phylogenetic analysis, we will study amphibian visual ecology and evolution, focusing especially on taxa that differ in key ecological traits, including diel activity and diet, and the changes the visual system undergoes during metamorphosis. We will also study the evolution of amphibian skin, focusing specifically on the morphology of glands used in breeding and chemical defense. A central analytical goal will be to understand the impact, relative to both method of DNA sequence alignment and phylogenetic optimality criterion, of comparatively small phenomic data partitions on the results of total evidence phylogenetic analyses when combined with much larger genomic data partitions. We will employ comparative and experimental approaches to document and explain variation in lipophilic alkaloid-based and tetrodotoxin-based chemical defenses at different temporal and spatial scales. Finally, we will install audio recording stations for long-term, continuous monitoring of anuran populations in stream, pond, and forest habitats within the Boracéia Biological Station, enabling us to quantify diel, seasonal, annual, and long-term variation in amphibian activity at each site. (AU)

Scientific publications

(17)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FAIVOVICH, JULIAN; PINHEIRO, PAULO D. P.; LYRA, MARIANA L.; PEREYRA, MARTIN O.; BALDO, DIEGO; MUNOZ, ARTURO; REICHLE, STEFFEN; BRANDAO, REUBER A.; GIARETTA, ARIOVALDO A.; THOME, MARIA TEREZA C.; CHAPARRO, JUAN C.; BAETA, DELIO; WIDHOLZER, RONALDO LIBARDI; BALDO, JORGE; LEHR, EDGAR; WHEELER, WARD C.; GARCIA, PAULO C. A.; HADDAD, CELIO F. B. Phylogenetic relationships of the Boana pulchella Group (Anura: Hylidae). Molecular Phylogenetics and Evolution, v. 155, FEB 2021. Web of Science Citations: 0.

NOVAES-E-FAGUNDES, GABRIEL; ARAUJO-VIEIRA, KATYUSCIA; ENTIAUSPE-NETO, OMAR M.; ROBERTO, IGOR J.; ORRICO, VICTOR G. D.; SOLE, MIRCO; HADDAD, CELIO F. B.; LOEBMANN, DANIEL. A new species of Scinax Wagler (Hylidae: Scinaxini) from the tropical forests of Northeastern Brazil. Zootaxa, v. 4903, n. 1, p. 1-41, JAN 6 2021. Web of Science Citations: 0.

MACHADO, DENIS JACOB; CASTROVIEJO-FISHER, SANTIAGO; GRANT, TARAN. Evidence of absence treated as absence of evidence: The effects of variation in the number and distribution of gaps treated as missing data on the results of standard maximum likelihood analysis. Molecular Phylogenetics and Evolution, v. 154, JAN 2021. Web of Science Citations: 1.

LYRA, MARIANA L.; LOURENCO, ANA CAROLINA C.; PINHEIRO, PAULO D. P.; PEZZUTI, TIAGO L.; BAETA, DELIO; BARLOW, AXEL; HOFREITER, MICHAEL; POMBAL JR, JOSE P.; HADDAD, CELIO F. B.; FAIVOVICH, JULIAN. High-throughput DNA sequencing of museum specimens sheds light on the long-missing species of the Bokermannohyla claresignata group (Anura: Hylidae: Cophomantini). ZOOLOGICAL JOURNAL OF THE LINNEAN SOCIETY, v. 190, n. 4, p. 1235-1255, DEC 2020. Web of Science Citations: 2.

VERA CANDIOTI, FLORENCIA; GROSSO, JIMENA; PEREYRA, MARTIN O.; HAAD, M. BELEN; LESCANO, JULIAN; SIU-TING, KAREN; AGUILAR, CESAR; BALDO, DIEGO. Larval Anatomy of Andean Toads of the Rhinella spinulosa Group (Ahura: Bufonidae). HERPETOLOGICAL MONOGRAPHS, v. 34, p. 116-130, DEC 2020. Web of Science Citations: 0.

CHAVES-ACUNA, WAGNER; CHAVES, GERARDO; KLANK, JEREMY; ARIAS, ERICK; BOLANOS, FEDERICO; SHEPACK, ALEX; LEENDERS, TWAN; COSSEL, JOHN; FAIVOVICH, JULIAN. Recent findings of Isthmohyla pictipes (Anura: Hylidae) in Costa Rica: variation and implications for conservation. Zootaxa, v. 4881, n. 3, p. 499-514, NOV 20 2020. Web of Science Citations: 0.

ORRICO, VICTOR G. D.; GRANT, TARAN; FAIVOVICH, JULIAN; RIVERA-CORREA, MAURICIO; RADA, MARCO A.; LYRA, MARIANA L.; CASSINI, CARLA S.; VALDUJO, PAULA H.; SCHARGEL, WALTER E.; MACHADO, DENIS J.; WHEELER, WARD C.; BARRIO-AMOROS, CESAR; LOEBMANN, DANIEL; MORAVEC, JIRI; ZINA, JULIANA; SOLE, MIRCO; STURARO, MARCELO J.; PELOSO, PEDRO L. V.; SUAREZ, PABLO; HADDAD, CELIO F. B. The phylogeny of Dendropsophini (Anura: Hylidae: Hylinae). CLADISTICS, v. 37, n. 1 SEP 2020. Web of Science Citations: 0.

TABOADA, CARLOS; BRUNETTI, ANDRES E.; LYRA, MARIANA L.; FITAK, ROBERT R.; FAIGON SOVERNA, ANA; RON, SANTIAGO R.; LAGORIO, MARIA G.; HADDAD, CELIO F. B.; LOPES, NORBERTO P.; JOHNSEN, SONKE; FAIVOVICH, JULIAN; CHEMES, LUCIA B.; BARI, SARA E. Multiple origins of green coloration in frogs mediated by a novel biliverdin-binding serpin. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v. 117, n. 31, p. 18574-18581, AUG 4 2020. Web of Science Citations: 2.

DIAS, PEDRO HENRIQUE DOS SANTOS. The remarkable larval anatomy ofProceratophrys minutaNapoli, Cruz, Abreu andDel-Grande,2011(Amphibia: Anura: Odontophrynidae). Journal of Morphology, v. 281, n. 9 JUL 2020. Web of Science Citations: 1.

JECKEL, ADRIANA M.; MATSUMURA, KUNIHIRO; NISHIKAWA, KEISUKE; MORIMOTO, YOSHIKI; SAPORITO, RALPH A.; GRANT, TARAN; IFA, DEMIAN R. Use of whole-body cryosectioning and desorption electrospray ionization mass spectrometry imaging to visualize alkaloid distribution in poison frogs. Journal of Mass Spectrometry, v. 55, n. 6 JUN 2020. Web of Science Citations: 0.

ARAUJO-VIEIRA, KATYUSCIA; LUNA, MARIA CELESTE; CARAMASCHI, ULISSES; HADDAD, CELIO F. B. A new genus of lime treefrogs (Anura: Hylidae: Sphaenorhynchini). ZOOLOGISCHER ANZEIGER, v. 286, p. 81-89, MAY 2020. Web of Science Citations: 0.

BLOTTO, BORIS L.; LYRA, MARIANA L.; CARDOSO, MONICA C. S.; RODRIGUES, MIGUEL TREFAUT; DIAS, IURI; MARCIANO-JR, EUVALDO; DAL VECHIO, FRANCISCO; ORRICO, VICTOR G. D.; BRANDAO, REUBER A.; DE ASSIS, CLODOALDO LOPES; LANTYER-SILVA, AMANDA S. F.; RUTHERFORD, MIKE G.; GAGLIARDI-URRUTIA, GIUSSEPE; SOLE, MIRCO; BALDO, DIEGO; NUNES, IVAN; CAJADE, RODRIGO; TORRES, AMBROSIO; GRANT, TARAN; JUNGFER, KARL-HEINZ; DA SILVA, HELIO R.; HADDAD, CELIO F. B.; FAIVOVICH, JULIAN. The phylogeny of the Casque-headed Treefrogs (Hylidae: Hylinae: Lophyohylini). CLADISTICS, v. 37, n. 1 MAR 2020. Web of Science Citations: 6.

YOVANOVICH, CAROLA A. M.; PIEROTTI, MICHELE E. R.; KELBER, ALMUT; JORGEWICH-COHEN, GABRIEL; IBANEZ, ROBERTO; GRANT, TARAN. Lens transmittance shapes ultraviolet sensitivity in the eyes of frogs from diverse ecological and phylogenetic backgrounds. PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, v. 287, n. 1918 JAN 15 2020. Web of Science Citations: 0.

KATYUSCIA ARAUJO-VIEIRA; JOSÉ P. POMBAL JR.; ULISSES CARAMASCHI; GABRIEL NOVAES-E-FAGUNDES; VICTOR G.D. ORRICO; JULIÁN FAIVOVICH. A neotype for Hyla x-signata Spix, 1824 (Amphibia, Anura, Hylidae). Papéis Avulsos de Zoologia (São Paulo), v. 60, p. -, 2020.

MAILHO-FONTANA, PEDRO LUIZ; JARED, CARLOS; ANTONIAZZI, MARTA MARIA; SCIANI, JULIANA MOZER; ARVALHO PIMENTA, DANIEL C.; STOKES, AMBER N.; GRANT, TARAN; BRODIE, III, EDMUND D.; BRODIE, JR., EDMUND D. Variations in tetrodotoxin levels in populations of Taricha granulosa are expressed in the morphology of their cutaneous glands. SCIENTIFIC REPORTS, v. 9, DEC 6 2019. Web of Science Citations: 0.

PINHEIRO, PAULO D. P.; CARRIZO, GUSTAVO R.; FAIVOVICH, JULIAN. The identity of the poorly known treefrog Hyla varelae Carrizo, 1992 (Anura: Hylidae). ZOOLOGISCHER ANZEIGER, v. 283, p. 186-191, NOV 2019. Web of Science Citations: 0.

GRANT, TARAN. Outgroup sampling in phylogenetics: Severity of test and successive outgroup expansion. JOURNAL OF ZOOLOGICAL SYSTEMATICS AND EVOLUTIONARY RESEARCH, v. 57, n. 4 JULY 2019. Web of Science Citations: 1.