Study of the effects of progesterone and the hypercaloric diet on the interactions between pancreatic beta cell and adipose tissue

• Grant number: 18/22361-9
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2019
• End date: May 31, 2022
• Field of knowledge: Biological Sciences - Physiology - General Physiology
• Principal Investigator: Anna Karenina Azevedo Martins
• Grantee: Anna Karenina Azevedo Martins
• Host Institution: Escola de Artes, Ciências e Humanidades (EACH). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Fabiana de Sant'Anna Evangelista ; Sandra Coccuzzo Sampaio Vessoni
• Associated scholarship(s): 20/03724-3 - Study of the effects of progesterone and the hypercaloric diet on physiological aspects of female mice, BP.TT

Abstract

Obesity has been taking on alarming dimensions at the global level, which reflects changes in dietary and nutritional patterns. Associated with the pathogenesis of this condition is the increased peripheral resistance to insulin, which may be related to the increased risk that obese women have to develop gestational diabetes. Pregnancy, per se, is a condition where physiological resistance to insulin isobserved and progesterone is one of the hormones responsible for this condition.Considering this scenario, here we intend to investigate the effects of progesteroneand the hypercaloric diet on the interactions between pancreatic beta cell and adipose tissue. Two approaches will be used. In vivo, female C57B1 mice fed standard or hypercaloric diet will be used, and followed up general and metabolic parameters, such as: body weight, energy expenditure, fasting glucose, insulin and glucose tolerance tests. Immunohistochemical analyzes of adipose tissue and pancreas, study of insulin secretion by isolated islets and plasma adiponectindetermination will also be performed. In the in vitro approach, beta cell lines, RINm5Fand INS-1E, will be used to analyze the combined effects of fatty acids and steroidhormones on cell parameters such as: cell membrane integrity, percentage of cellswith fragmented DNA, cell proliferation and analysis of the production of reactivespecies. Thus, we hope to contribute with data on the effects of progesterone on thecommunication between beta cells and adipose tissue, so that at the end of this workwe will understand a little more about the relationship between obesity and DG. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

AZEVEDO-MARTINS, ANNA; BOSCHETTI, DANIELA; MULLER, CYNTHIA; AMERICO, ANNA; VECCHIATTO, BRUNO; MARTUCCI, LUIZ; PEREIRA, RENATA; OLIVEIRA, CLAUDIA; EVANGELISTA, FABIANA. Potential of Aerobic Physical Training to Minimize the Effects of Leptin Deficiency in Female Mice. FASEB JOURNAL, v. 34, p. 2-pg., 2020-04-01. (18/22361-9)

AZEVEDO-MARTINS, ANNA K.; FERREIRA, MARILIA M.; CAUDURO, LEONARDO R.; DOS SANTOS, MATHEUS P.; VECCHIATTO, BRUNO; MARTUCCI, LUIZ F. MARTUCCI; ROGERO, MARCELO M.; EVANGELISTA, FABIANA DE SANT'ANNA. Progesterone Treatment Attenuates Body Weight Gain, Adiposity and Glycaemic Metabolism Damage in Obese Female Mice. FASEB JOURNAL, v. 36, p. 1-pg., 2022-01-01. (18/22361-9)