Transient response of inflammatory mediators induced by lipopolysaccharide in blood and allantoic fluid of pregnant mares.

• Grant number: 19/03193-0
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2019
• End date: December 31, 2021
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Renata Gebara Sampaio Dória
• Grantee: Renata Gebara Sampaio Dória
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
• Associated researchers: Adriana Helena de Souza ; Carlos Augusto Araújo Valadão ; Fernando de Queiroz Cunha ; Juliana Regina Peiró ; Luciano Andrade Silva

Abstract

Endotoxemia is a frequent consequence of gastrointestinal diseases in horses and leads to the activation of the inflammatory cascade, including the release of proinflammatory cytokines, from pathogen recognition and pathogen-associated molecular patterns (PAMPS), through (PRR), which detect a specific ligand, such as lipopolysaccharide (LPS), the main endotoxin related to this inflammatory condition in horses. Studies have reported effects of maternal colic on the equine fetus and associated abortions with signs of endotoxemia in mares in late gestation, suggesting the influence of this systemic syndrome on the fetus. Elevated levels of endotoxins and inflammatory cytokines have been demonstrated in the amniotic fluid of women with membrane rupture and preterm labor. The fetoplacental unit is a dynamic system of exchange between maternal and fetal fluids. In human medicine, amniotic fluid evaluation is widely used to analyze fetal viability. However, in equines, the physiology of fetal fluids and their chemical composition is poorly understood and few studies have been developed for this purpose, especially with diseased pregnant mares. This work aims to determine and quantify inflammatory mediators, nuclear factor NFº-B activity and gene expression of the TLR-4 receptor in the blood and fetal fluids of mares in late gestation during maternal systemic endotoxemia induced by intravenous infusion of LPS, in order to understand the pathophysiology of endotoxemia in pregnant mares and their influence on the fetus. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GARCIA, ADRIANA F. S.; DORIA, RENATA G. S.; ARANTES, JULIA A.; REGINATO, GUSTAVO M.; NEUBAUER, FERNANDA G.; RIBEIRO, GESIANE. Intravenous Regional Limb Perfusion in Standing and Recumbent Horses: A Comparative Radiographic Study. Journal of Equine Veterinary Science, v. 98, MAR 2021. (19/03193-0, 15/21430-9)

VIVIAN C.M. PRADO; MARIA CRISTINA F.N.S. HAGE; RENATA G.S. DÓRIA. Bem-estar e afecções do aparelho locomotor de equinos de tração (carroceiros) em atividade. Pesquisa Veterinária Brasileira, v. 39, n. 12, p. 942-948, 2020-01-10. (19/03193-0, 15/12009-8)

FERREIRA, MARILIA ALVES; AGUIAR, MAYRA LISSONI; GRIGOLETTO, RENAN; PASSARELLI, DANIELLE; ARANTES, JULIA DE ASSIS; REGINATO, GUSTAVO MORANDINI; SALLES BRITO, PEDRO HENRIQUE; FERNANDES DA SILVA, THIAGO JONATHA; SAMPAIO DORIA, RENATA GEBARA. Comparison of plasma and intra-articular gentamicin levels after intravenous administration in healthy horses. ACTA VETERINARIA BRNO, v. 89, n. 3, p. 225-230, 2020. (19/03193-0, 13/20662-8)

BIANCA MOUTINHO GRIZENDI; RENATA GEBARA SAMPAIO DÓRIA; DANIELE PASSARELLI; GUSTAVO MORANDINI REGINATO; YUMI DE BARCELOS HAYASAKA; PAULO FANTINATO NETO. Correlação entre avaliação hematológica e tipo de atividade física realizada por equinos no estado de São Paulo-Brasil. Ciênc. anim. bras., v. 21, 2020-06-01. (19/03193-0, 12/12002-5)

DE SOUZA GARCIA, ADRIANA FERNANDES; RIBEIRO, GESIANE; ARANTES, JULIA DE ASSIS; REGINATO, GUSTAVO MORANDINI; XAVIER, NATHALIA VILLACA; CARREGARO, ADRIANO BONFIM; FERNANDES SILVA, THIAGO JHONATHA; GRIGOLETTO, RENAN; DE FREITAS, SILVIO HENRIQUE; SAMPAIO DORIA, RENATA GEBARA. Evaluation of totally implantable catheters in healthy horses. BMC Veterinary Research, v. 17, n. 1, OCT 26 2021. (19/03193-0, 15/21430-9)