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Transcriptome of the bacterial chemical signaling in Salmonella Typhimurium, emergent global bacterial enteropathogens investigation in São Paulo, and alternative infection model employed to study pathogenesis


Bacterial chemical signaling is a mechanism employed by several pathogenic species to interact with the host and surrounding microbiota. Upon this interaction the pathogenic bacteria regulate their virulence traits such as QseBC and VisP/YgiV. The 2-component system QseBC and the chemical signaling of the Autoinducer-3, epinephrine, and norepinephrine in Salmonella enterica serovar Typhimurium have opened a novel perspective to understand pathogenic mechanisms. Amongst these mechanisms the protein VisP (Virulence and stress-related Periplasmic protein) was described, interacting with LpxO enzyme (Dioxygenase Fe2+/ ±-ketoglutarate-dependent), and its role during the O-antigen assembly via Wzz system in S. Typhimurium was studied and elucidated by our group, and YgiV is a potential AraC-like regulator, but its function is not completely defined. Mechanism details still need to be assessed, the transcriptomic analysis of YgiV, QseC, and VisP as well as the intriguing genic regulation of qseB/qseC and visP/ygiV operons will help to unravel novel mechanisms during S. Typhimurium pathogenesis and other correlated pathogens. In parallel, the study will explore the novel pathogens such as the clonal type ST313 to elucidate details of these highly infective diarrheagenic strains in São Paulo, and its transcriptomic is going to address the gastrointestinal barrier break to reach bloodstream of these patients. The study will also employ innovative and alternate models to study infection in vivo, such as Galleria mellonella to better evaluate the pathogenic bacterial processes.The full comprehension of the complex relationship and the regulation is essential among bacteria in complex communities such as human gut, that albeit a vast and complex microbiome. The chemical signaling among bacteria and the host is the central aspect of this association, the project will help to understand their relationship and future development of novel technologies and therapies in benefit to human life. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BRAZ, VANIA SANTOS; MELCHIOR, KARINE; MOREIRA, CRISTIANO GALLINA. Escherichia coli as a Multifaceted Pathogenic and Versatile Bacterium. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 10, DEC 21 2020. Web of Science Citations: 0.
SIMEI AQUARONI, NAYARA AP.; NAKAHATA, DOUGLAS H.; LAZARINI, SILMARA C.; RESENDE, FLAVIA A.; CANDIDO, AMANDA L. P.; BARUD, HERNANE DA SILVA; CLARO, AMANDA MARIA; DE CARVALHO, JOAO ERNESTO; RIBEIRO, CAMILA M.; PAVAN, FERNANDO R.; LUSTRI, BRUNA C.; MACHADO RIBEIRO, TAMARA RENATA; MOREIRA, CRISTIANO G.; CANDIDO, TUANY ZAMBROTI; PASSOS LIMA, CARMEN SILVIA; RUIZ, ANA LUCIA T. G.; CORBI, PEDRO P.; LUSTRI, WILTON R. Antibacterial activities and antiproliferative assays over a tumor cells panel of a silver complex with 4-aminobenzoic acid: Studies in vitro of sustained release using bacterial cellulose membranes as support. Journal of Inorganic Biochemistry, v. 212, NOV 2020. Web of Science Citations: 0.

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