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BISPHOSPHONATES AND IMPLANTOLOGY: EFFECT OF METALLOPROTEINASE INHIBITORS ON IN VITRO MODEL

Grant number: 18/11211-6
Support type:Regular Research Grants
Duration: June 01, 2019 - May 31, 2022
Field of knowledge:Health Sciences - Dentistry - Periodontology
Principal researcher:Fernanda Gonçalves Basso
Grantee:Fernanda Gonçalves Basso
Home Institution: Universidade de Ribeirão Preto (UNAERP). Campus Ribeirão Preto. Ribeirão Preto , SP, Brazil
Assoc. researchers:Carlos Alberto de Souza Costa

Abstract

Bisphosphonates are indicated for treatment of bone metabolic and neoplastic diseases aiming to control the resorption of this tissue. These drugs show high affinity to mineral tissue and are released after a traumatic or inflammatory stimulus. Once released, bisphosphonates act by inducing apoptosis of osteoclasts and inhibiting the maturation of these cells. The installation of osseointegrated implants on patients under treatment with these drugs show controversial clinical outcomes. After installation of oral implants, the local trauma may result in osteonecrotic areas. Recent studies demonstrated that these outcomes might vary from improved osseointegration or even to implant loss. However, few is known about the factors that may act on these events. One of these factors is the type and administration of bisphosphonates. These factors may influence on the response of bone and mucous tissue, since these drugs may negatively affect the metabolism of these cells, inhibiting osseointegration and biological sealing formation. The super-expression of metalloproteinases may also interefere on these events. So, the modulation of these enzymes could favor these cellular and tissue events. Therefore, the aim of this study will be to evaluate the effect of two inhibitors of metalloproteinases - proanthocyanidin and naringenin, on the adhesion of osteoblasts and fibroblasts on titanium surfaces as also the genic expression, synthesis and activity of metalloproteinases by these cells subjected to treatment with zoledronic acid at different concentrations. Titanium discs will be placed on 24-well cell culture plates and cells will be seeded onto these discs. Then, after 24 hours, zoledronic acid will be added in fresh serum-free DMEM at 0,5, 1 e 5uM. AFter 24 and 48 hours cell proliferation (alamarblue), adhesion (fluorescence) and gene expression and synthesis (real time PCR and ELISA) will be determined. Gelatinolytic activity will be evaluated by in situ zymography. (AU)

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Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PANSANI, TAISA NOGUEIRA; CARDOSO, LAIS MEDEIROS; AUGUSTO, LUISA AMMIRABILE; RIBEIRO, ISABELA MASSARO; DE SOUZA COSTA, CARLOS ALBERTO; BASSO, FERNANDA GONCALVES. Effects of EGF-coated titanium surfaces on adhesion and metabolism of bisphosphonate-treated human keratinocytes and gingival fibroblasts. CLINICAL ORAL INVESTIGATIONS, v. 25, n. 10, p. 5775-5784, OCT 2021. Web of Science Citations: 0.
BASSO, FERNANDA GONCALVES; CARDOSO, LAIS MEDEIROS; RIBEIRO, ISABELA MASSARO; RIZZI, ELLEN; PANSANI, TAISA NOGUEIRA; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. Influence of bisphosphonates on oral implantology: Sodium alendronate and zoledronic acid enhance the synthesis and activity of matrix metalloproteinases by gingival fibroblasts seeded on titanium. ARCHIVES OF ORAL BIOLOGY, v. 127, JUL 2021. Web of Science Citations: 0.
CARDOSO, LAIS MEDEIROS; PANSANI, TAISA NOGUEIRA; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO; BASSO, FERNANDA GONCALVES. Chemotherapy drugs and inflammatory cytokines enhance matrix metalloproteinases expression by oral mucosa cells. ARCHIVES OF ORAL BIOLOGY, v. 127, JUL 2021. Web of Science Citations: 0.
PANSANI, TAISA NOGUEIRA; CARDOSO, LAIS MEDEIROS; AUGUSTO, LUISA AMMIRABILE; RIBEIRO, ISABELA MASSARO; DE SOUZA COSTA, CARLOS ALBERTO; BASSO, FERNANDA GONCALVES. Effects of EGF-coated titanium surfaces on adhesion and metabolism of bisphosphonate-treated human keratinocytes and gingival fibroblasts. CLINICAL ORAL INVESTIGATIONS, APR 2021. Web of Science Citations: 0.
FERNANDA G. BASSO; TAISA N. PANSANI; LAIS M. CARDOSO; JOSIMERI HEBLING; RODRIGO PAES VILA REAL; CARLOS ALBERTO DE SOUZA COSTA. Influence of Bisphosphonates on the Behavior of Osteoblasts Seeded Onto Titanium Discs. Brazilian Dental Journal, v. 31, n. 3, p. 304-309, Jun. 2020.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.