Research Grants 18/11211-6 - Metaloproteinases, Implantodontia - BV FAPESP
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BISPHOSPHONATES AND IMPLANTOLOGY: EFFECT OF METALLOPROTEINASE INHIBITORS ON IN VITRO MODEL

Grant number: 18/11211-6
Support Opportunities:Regular Research Grants
Start date: June 01, 2019
End date: May 31, 2022
Field of knowledge:Health Sciences - Dentistry - Periodontology
Principal Investigator:Fernanda Gonçalves Basso
Grantee:Fernanda Gonçalves Basso
Host Institution: Universidade de Ribeirão Preto (UNAERP). Campus Ribeirão Preto. Ribeirão Preto , SP, Brazil
Associated researchers:Carlos Alberto de Souza Costa

Abstract

Bisphosphonates are indicated for treatment of bone metabolic and neoplastic diseases aiming to control the resorption of this tissue. These drugs show high affinity to mineral tissue and are released after a traumatic or inflammatory stimulus. Once released, bisphosphonates act by inducing apoptosis of osteoclasts and inhibiting the maturation of these cells. The installation of osseointegrated implants on patients under treatment with these drugs show controversial clinical outcomes. After installation of oral implants, the local trauma may result in osteonecrotic areas. Recent studies demonstrated that these outcomes might vary from improved osseointegration or even to implant loss. However, few is known about the factors that may act on these events. One of these factors is the type and administration of bisphosphonates. These factors may influence on the response of bone and mucous tissue, since these drugs may negatively affect the metabolism of these cells, inhibiting osseointegration and biological sealing formation. The super-expression of metalloproteinases may also interefere on these events. So, the modulation of these enzymes could favor these cellular and tissue events. Therefore, the aim of this study will be to evaluate the effect of two inhibitors of metalloproteinases - proanthocyanidin and naringenin, on the adhesion of osteoblasts and fibroblasts on titanium surfaces as also the genic expression, synthesis and activity of metalloproteinases by these cells subjected to treatment with zoledronic acid at different concentrations. Titanium discs will be placed on 24-well cell culture plates and cells will be seeded onto these discs. Then, after 24 hours, zoledronic acid will be added in fresh serum-free DMEM at 0,5, 1 e 5uM. AFter 24 and 48 hours cell proliferation (alamarblue), adhesion (fluorescence) and gene expression and synthesis (real time PCR and ELISA) will be determined. Gelatinolytic activity will be evaluated by in situ zymography. (AU)

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Scientific publications (11)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PANSANI, TAISA NOGUEIRA; CARDOSO, LAIS MEDEIROS; AUGUSTO, LUISA AMMIRABILE; RIBEIRO, ISABELA MASSARO; DE SOUZA COSTA, CARLOS ALBERTO; BASSO, FERNANDA GONCALVES. Effects of EGF-coated titanium surfaces on adhesion and metabolism of bisphosphonate-treated human keratinocytes and gingival fibroblasts. CLINICAL ORAL INVESTIGATIONS, . (15/19364-8, 18/11211-6)
REAL, RODRIGO PAES VILA; PANSANI, TAISA NOGUEIRA; CARDOSO, LAIS MEDEIROS; COSTA, CARLOS ALBERTO DE SOUZA; BASSO, FERNANDA GONCALVES. Titanium alkalinization improves response of osteoblasts to zoledronic acid. BIOINTERPHASES, v. 17, n. 3, p. 8-pg., . (18/11211-6)
CARDOSO, LAIS MEDEIROS; PANSANI, TAISA NOGUEIRA; DE SOUZA COSTA, CARLOS ALBERTO; BASSO, FERNANDA GONCALVES. Regulation of interleukin-6 and matrix metalloproteinases syntheses by bioflavonoids and photobiomodulation in human gingival fibroblasts. Lasers in Medical Science, v. N/A, p. 15-pg., . (18/11211-6, 19/16886-4)
PANSANI, TAISA NOGUEIRA; CARDOSO, LAIS MEDEIROS; AUGUSTO, LUISA AMMIRABILE; RIBEIRO, ISABELA MASSARO; DE SOUZA COSTA, CARLOS ALBERTO; BASSO, FERNANDA GONCALVES. Effects of EGF-coated titanium surfaces on adhesion and metabolism of bisphosphonate-treated human keratinocytes and gingival fibroblasts. CLINICAL ORAL INVESTIGATIONS, v. 25, n. 10, p. 5775-5784, . (15/19364-8, 18/11211-6)
CARDOSO, LAIS MEDEIROS; PANSANI, TAISA NOGUEIRA; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO; BASSO, FERNANDA GONCALVES. Chemotherapy drugs and inflammatory cytokines enhance matrix metalloproteinases expression by oral mucosa cells. ARCHIVES OF ORAL BIOLOGY, v. 127, p. 8-pg., . (19/16886-4, 18/11211-6)
BASSO, FERNANDA GONCALVES; CARDOSO, LAIS MEDEIROS; RIBEIRO, ISABELA MASSARO; RIZZI, ELLEN; PANSANI, TAISA NOGUEIRA; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. nfluence of bisphosphonates on oral implantology: Sodium alendronate and zoledronic acid enhance the synthesis and activity of matrix metalloproteinases by gingival fibroblasts seeded on titaniu. ARCHIVES OF ORAL BIOLOGY, v. 127, . (18/11211-6, 19/16886-4)
CARDOSO, LAIS MEDEIROS; PANSANI, TAISA NOGUEIRA; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO; BASSO, FERNANDA GONCALVES. hemotherapy drugs and inflammatory cytokines enhance matrix metalloproteinases expression by oral mucosa cell. ARCHIVES OF ORAL BIOLOGY, v. 127, . (18/11211-6, 19/16886-4)
FERNANDA G. BASSO; TAISA N. PANSANI; LAIS M. CARDOSO; JOSIMERI HEBLING; RODRIGO PAES VILA REAL; CARLOS ALBERTO DE SOUZA COSTA. Influence of Bisphosphonates on the Behavior of Osteoblasts Seeded Onto Titanium Discs. Brazilian Dental Journal, v. 31, n. 3, p. 304-309, . (18/11211-6)
CARDOSO, LAIS MEDEIROS; PANSANI, TAISA NOGUEIRA; COSTA, CARLOS ALBERTO DE SOUZA; BASSO, FERNANDA GONCALVES. Naringenin and proanthocyanidins pre-treatment decreases synthesis and activity of gelatinases induced by zoledronic acid in a dental implant surface in vitro model. ARCHIVES OF ORAL BIOLOGY, v. 151, p. 10-pg., . (19/16886-4, 18/11211-6)
BASSO, FERNANDA GONCALVES; CARDOSO, LAIS MEDEIROS; RIBEIRO, ISABELA MASSARO; RIZZI, ELLEN; PANSANI, TAISA NOGUEIRA; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. Influence of bisphosphonates on oral implantology: Sodium alendronate and zoledronic acid enhance the synthesis and activity of matrix metalloproteinases by gingival fibroblasts seeded on titanium. ARCHIVES OF ORAL BIOLOGY, v. 127, p. 6-pg., . (19/16886-4, 18/11211-6)
MOURA NETO, JOAO; CARDOSO, LAIS MEDEIROS; PANSANI, TAISA NOGUEIRA; RAUCCI, LARISSA M. S. C.; COSTA, CARLOS ALBERTO DE SOUZA; BASSO, FERNANDA G.. Influence of titanium and zirconia substrates on the synthesis of inflammatory mediators. BIOINTERPHASES, v. 18, n. 4, p. 7-pg., . (18/11211-6)

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