Karyotype and sex chromosome evolution in Neotropical fishes. Part III.

• Grant number: 18/24235-0
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2019
• End date: May 31, 2021
• Field of knowledge: Biological Sciences - Genetics - Animal Genetics
• Principal Investigator: Luiz Antonio Carlos Bertollo
• Grantee: Luiz Antonio Carlos Bertollo
• Host Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
• Associated researchers: Eliana Feldberg ; Manoela Maria Marinho Koh ; Manolo Fernandez Perez ; Marcelo de Bello Cioffi ; Orlando Moreira Filho ; Terumi Hatanaka

Abstract

The Neotropical freshwater fish fauna is one of the most speciose in the world, in addition to several species to be described yet. In this way, such enormous diversity constitutes a challenge for scientific knowledge, offering many fundamental evolutionary questions to be solved. In this scenario, the fish cytogenetics has provided important advances by using both classical and molecular procedures. In turn, some fish families have been found as useful investigative models, enabling advances concerning our real biodiversity, evolutionary processes, and interrelationships. Some of them have been studied by our research group for years, and new and important data in relation to cryptic species complexes and the differentiation of sex chromosomes in lower vertebrates were found. In this context, the present proposal has in mind to give continuity to the former projects (FAPESP- 2014/22532-7 e FAPESP- 2016/21411-7), focusing now in some fish groups not deeply studied yet, but also promising for evolutionary studies such as the Lebiasinidae and Ctenolucidae families, emphasizing on specific genomic markers using fluorescence in situ hybridization (FISH), chromosomal microdissection, whole chromosome painting (WCP) and comparative genomic hybridization (CGH). Such procedures will be directed to itemized analyses of the karyotype organization of species from these two fish families, their probable relationships and the differentiation process of the sex chromosomes that they harbor. Partnerships with Brazilian and European researcher groups will be strengthened, as well as the scientific training of undergraduate, master, doctoral and post-doctoral students. Resolute advances in respect to the characterization of the biodiversity and relationships among our fish species are expected, on the basis of the proposals that are being presented and the robust partnerships that we have adopted and maintained in the last years in our research group. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CAVALCANTE SASSI, FRANCISCO DE MENEZES; DE OLIVEIRA, EZEQUIEL AGUIAR; CARLOS BERTOLLO, LUIZ ANTONIO; NIRCHIO, MAURO; HATANAKA, TERUMI; FERREIRA MARINHO, MANOELA MARIA; MOREIRA-FILHO, ORLANDO; AROUTIOUNIAN, ROUBEN; LIEHR, THOMAS; AL-RIKABI, AHMED B. H.; et al. Chromosomal Evolution and Evolutionary Relationships of Lebiasina Species (Characiformes, Lebiasinidae). INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 20, n. 12, JUN 2 2019. (17/09321-5, 18/22033-1, 18/24235-0)

SASSI, FRANCISCO DE M. C.; HATANAKA, TERUMI; DE MORAE, RENATA LUIZA R.; TOMA, GUSTAVO A.; DE OLIVEIRA, EZEQUIEL A.; LIEHR, THOMAS; RAB, PETR; BERTOLLO, LUIZ A. C.; VIANA, PATRIK F.; FELDBERG, ELIANA; et al. An Insight into the Chromosomal Evolution of Lebiasinidae (Teleostei, Characiformes). GENES, v. 11, n. 4, APR 2020. (17/09321-5, 18/22033-1, 18/24235-0)

TOMA, GUSTAVO AKIRA; ROSA DE MORAES, RENATA LUIZA; CAVALCANTE SASSI, FRANCISCO DE MENEZES; CARLOS BERTOLLO, LUIZ ANTONIO; DE OLIVEIRA, EZEQUIEL AGUIAR; RAB, PETR; SEMBER, ALEXANDR; LIEHR, THOMAS; HATANAKA, TERUMI; VIANA, PATRIK FERREIRA; et al. Cytogenetics of the small-sized fish, Copeina guttata (Characiformes, Lebiasinidae): Novel insights into the karyotype differentiation of the family. PLoS One, v. 14, n. 12, DEC 19 2019. (18/22033-1, 18/24235-0, 17/09321-5)

SEMBER, ALEXANDR; DE OLIVEIRA, EZEQUIEL AGUIAR; RAB, PETR; BERTOLLO, LUIZ ANTONIO CARLOS; DE FREITAS, NATALIA LOURENCO; VIANA, PATRIK FERREIRA; YANO, CASSIA FERNANDA; HATANAKA, TERUMI; MARINHO, MANOELA MARIA FERREIRA; DE MORAES, RENATA LUIZA ROSA; et al. Centric Fusions behind the Karyotype Evolution of Neotropical Nannostomus Pencilfishes (Characiforme, Lebiasinidae): First Insights from a Molecular Cytogenetic Perspective. GENES, v. 11, n. 1, JAN 2020. (17/09321-5, 18/24235-0)

CAVALCANTE SASSI, FRANCISCO DE MENEZES; DE OLIVEIRA, EZEQUIEL AGUIAR; CARLOS BERTOLLO, LUIZ ANTONIO; NIRCHIO, MAURO; HATANAKA, TERUMI; FERREIRA MARINHO, MANOELA MARIA; MOREIRA-FILHO, ORLANDO; AROUTIOUNIAN, ROUBEN; LIEHR, THOMAS; AL-RIKABI, AHMED B. H.; et al. Chromosomal Evolution and Evolutionary Relationships of Lebiasina Species (Characiformes, Lebiasinidae). INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 20, n. 12, p. 17-pg., 2019-06-02. (18/24235-0, 18/22033-1, 17/09321-5)

DE M. C. SASSI, FRANCISCO; PEREZ, MANOLO F.; OLIVEIRA, VANESSA CRISTINA S.; DEON, GEIZE A.; DE SOUZA, FERNANDO H. S.; FERREIRA, PEDRO H. N.; DE OLIVEIRA, EZEQUIEL A.; HATANAKA, TERUMI; LIEHR, THOMAS; BERTOLLO, LUIZ A. C.; et al. High Genetic Diversity despite Conserved Karyotype Organization in the Giant Trahiras from Genus Hoplias (Characiformes, Erythrinidae). GENES, v. 12, n. 2, FEB 2021. (18/24235-0, 18/22033-1, 20/02681-9)