Molecular aspects on host response to persistent apical periodontitis

• Grant number: 18/21130-3
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2019
• End date: November 30, 2022
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Manoel Damiao de Sousa Neto
• Grantee: Manoel Damiao de Sousa Neto
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• City of the host institution: Ribeirão Preto
• Associated researchers: Jardel Francisco Mazzi Chaves

Abstract

Genetic polymorphisms are differences in the human DNA sequence that can alter geneexpression and influence the organism's susceptibility to disease as well as its response to theenvironment. Thus, in addition to microbial and mechanical factors related to the root canalitself, intrinsic factors to the host may interfere with the success of endodontic treatment andthe need for reintervention. This study will investigate molecular aspects of periapical lesionsby means of histopathological and immunohistochemical evaluation, gene expression analysisby RT-PCR, as well as allelic discrimination by RT-PCR of saliva and cytokine dosing bymeans of an enzyme-linked immunosorbent assay (ELISA). The imaging aspects observed inthe development and progression of apical periodontitis using cone beam computedtomography (CBCT) will be used as phenotypic parameters to understand the molecularaspects involved in the etiology of persistent endodontic infection. Patients diagnosed withpulpal necrosis who, after endodontic treatment, still present persistent periapical lesions willbe selected for the study. Samples of saliva will be collected as a source of genomic DNAand, at the surgical moment, the periapical lesion will be collected. After the processing of thesamples, genotyping for RANK, RANKL, OPG, SOCS-1, SOCS-2, SOCS-3 and TNF-± willbe performed by RT-PCR, as well as histopathological classification andimmunohistochemical analysis, as well as the cytokine dosage by ELISA and gene expressionby RT-PCR. Parameters of shape, volume, area, perimeter, location and extension of thelesions will be obtained through the CBCT images. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ANTUNES, L. S.; CARVALHO, L.; PETEAN, I. B. F.; ANTUNES, L. A.; FREITAS, J. V.; SALLES, A. G.; OLEJ, B.; OLIVEIRA, D. S. B.; KUCHLER, E. C.; SOUSA-NETO, M. D.. Association between genetic polymorphisms in the promoter region of the defensin beta 1 gene and persistent apical periodontitis. International Endodontic Journal, v. 54, n. 1, p. 38-45, JAN 2021. (18/21130-3)

RAMIREZ, IAGO; KIRSCHNECK, CHRISTIAN; SILVA-SOUSA, ALICE CORREA; PROFF, PETER; ANTUNES, LEONARDO S.; GABBARDO, MARILISA C. L.; DE OLIVEIRA, DANIELA SILVA BARROSO; SOUSA-NETO, MANOEL D. D.; BARATTO-FILHO, FLARES; KUCHLER, ERIKA C. C.. The investigation of WNT6 and WNT10A single nucleotide polymorphisms as potential biomarkers for dental pulp calcification in orthodontic patients. PLoS One, v. 18, n. 8, p. 13-pg., 2023-08-11. (18/21130-3)

MEYFARTH, SANDRA REGINA SANTOS; ANTUNES, LIVIA AZEREDO ALVES; DA SILVA TAVARES, JHENYFER; GUIMARAES, LUDMILA DA SILVA; DA SILVA, ERLANGE ANDRADE BORGES; BARATTO-FILHO, FLARES; KUECHLER, ERIKA CALVANO; SILVA-SOUSA, ALICE CORREA; SOUSA-NETO, MANOEL DAMIAO; ANTUNES, LEONARDO SANTOS. Single nucleotide polymorphisms in inducible nitric oxide synthase gene are not associated with persistent apical periodontitis. AUSTRALIAN ENDODONTIC JOURNAL, v. N/A, p. 9-pg., 2023-09-19. (18/21130-3)

KUCHLER, ERIKA CALVANO; HANNEGRAF, NATASCHA DOUAT; LARA, RAFAELA MARIANA; BITENCOURT REIS, CAIO LUIZ; BARROSO DE OLIVEIRA, DANIELA SILVA; MAZZI-CHAVES, JARDEL FRANCISCO; RIBEIRO ANDRADES, KESLY MARY; DE LIMA, LORENA FERREIRA; SALLES, ALESSANDRO GUIMARAES; ALVES ANTUNES, LIVIA AZEREDO; et al. Investigation of Genetic Polymorphisms in BMP2, BMP4, SMAD6, and RUNX2 and Persistent Apical Periodontitis. JOURNAL OF ENDODONTICS, v. 47, n. 2, p. 278-285, FEB 2021. (15/06866-5, 18/21130-3)

PETEAN, IGOR BASSI FERREIRA; SILVA-SOUSA, ALICE CORREA; MARANON-VASQUEZ, GUIDO ARTEMIO; DE PAULA-SILVA, FRANCISCO WANDERLEY GARCIA; KUECHLER, ERIKA CALVANO; ANTUNES, LEONARDO SANTOS; SEGATO, RAQUEL ASSED BEZERRA; DA SILVA, LEA ASSED BEZERRA; MAZZI-CHAVES, JARDEL FRANCISCO; LOPES-OLHE, FABIANE CARNEIRO; et al. Interaction between polymorphisms in TNF-α and RANKL genes is associated with the development of persistent apical periodontitis, in Brazilian subjects. ARCHIVES OF ORAL BIOLOGY, v. 169, p. 8-pg., 2024-10-19. (18/21130-3)

GRACIANE E. R. QUEIROZ; THUANNY CASTILHO; LUDMILA SILVA GUIMARÃES; VANIA GOMES MORAES; ERLANGE ANDRADE BORGES DA SILVA; ERIKA CALVANO KÜCHLER; ALICE CORRÊA SILVA-SOUSA; MANOEL DAMIÃO SOUSA-NETO; LÍVIA AZEREDO ALVES ANTUNES; LEONARDO SANTOS ANTUNES. Influence of genetic polymorphisms on oral health-related quality of life after root canal treatment. Brazilian Dental Journal, v. 35, 2024-03-22. (18/21130-3)

PETEAN, IGOR BASSI FERREIRA; GAETA-ARAUJO, HUGO; MAZZI-CHAVES, JARDEL FRANCISCO; SILVA-SOUSA, ALICE CORREA; LOPES-OLHE, FABIANE CARNEIRO; DE PAULA-SILVA, FRANCISCO WANDERLEY GARCIA; DE SOUSA-NETO, MANOEL DAMIAO. Clinical and imaging aspects associated with persistent apical periodontitis: subsides for the treatment decision-making process. CLINICAL ORAL INVESTIGATIONS, v. 29, n. 1, p. 9-pg., 2025-01-21. (18/21130-3)