Enzymatic biotechnology for the xylo-oligosaccharides production from lignocellulosic agroindustrial residues aimed the production of symbiotics

• Grant number: 19/08542-3
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2019
• End date: July 31, 2022
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Engineering
• Principal Investigator: Rosana Goldbeck Coelho
• Grantee: Rosana Goldbeck Coelho
• Host Institution: Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated researchers: Ana Carla Kawazoe Sato

Abstract

Lignocellulosic materials represent a source of under-exploited raw material in biotechnological processes, since a large surplus of waste from the industrial processing of agricultural raw materials is generated annually. Thus, the production of xylo-oligosaccharides (XOs) from the enzymatic hydrolysis of xylan present in the hemicellulose from these residues presents a smart and sustainable alternative for their valorization, as well as the production of industrial inputs with high added value . Xylo-oligosaccharides (XOs) are distinguished by their prebiotic activities that favor an improvement in intestinal functions, immunological action, antimicrobial and other health benefits. In this sense, the present proposal aims to produce xylo-oligosaccharides from the enzymatic hydrolysis of agroindustrial lignocellulosic residues, aiming at the association with probiotic bacteria to obtain symbiotic microcapsules. In this work two agroindustrial residues from state of São Paulo will be tested: sugar cane straw and coffee husk. In a first stage the agroindustrial residues will be characterized as to the content of xylan present. Afterwards, experimental design will be carried out in order to select the enzymes that in fact have a significant impact on the release of XOs with polymerization degree (GP) of 2 to 6, as well as to optimize optimal concentrations of enzymes for the production of XOs. In parallel, a study will be carried out on the symbiotic association of XOs and probiotic bacteria, in order to obtain symbiotic microcapsules. By means of in vitro assays, the digestibility of the microcapsules obtained will be analyzed, which will be evaluated the resistance to the hydrolysis of enzymes of the digestive tract, as well as the stimulation of the growth of the probiotic bacteria. Finally, it is expected at the end of the project that the microcapsules produced from the xylo-oligosaccharides from lignocellulosic agroindustrial residues present symbiotic potential so that they can be applied industrially as functional ingredients in food products. It is important to mention that this line of research was initiated in the post-doctoral study of the researcher in question (responsible for the proposal) that was funded by FAPESP (12 / 18859-5) and is currently strengthening this line of research at School of Food Engineering - UNICAMP) where she is professor. (AU)

Scientific publications

(28)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

AZAMBUJA, SUELLEN P. H.; GOLDBECK, ROSANA. Butanol production by Saccharomyces cerevisiae: perspectives, strategies and challenges. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, v. 36, n. 3, MAR 9 2020. (19/08542-3, 15/20630-4)

BARBOSA, FERNANDO CESAR; KENDRICK, EMANUELE; BRENELLI, LIVIA BEATRIZ; ARRUDA, HENRIQUE SILVANO; PASTORE, GLAUCIA MARIA; RABELO, SARITA CANDIDA; DAMASIO, ANDRE; FRANCO, TELMA TEIXEIRA; LEAK, DAVID; GOLDBECK, ROSANA. Optimization of cello-oligosaccharides production by enzymatic hydrolysis of hydrothermally pretreated sugarcane straw using cellulolytic and oxidative enzymes. BIOMASS & BIOENERGY, v. 141, OCT 2020. (17/16159-0, 17/22669-0, 15/50612-8, 19/08542-3)

SANTOS, BRUNO A. S.; AZAMBUJA, SUELLEN P. H.; AVILA, PATRICIA F.; PACHECO, MARIA TERESA B.; GOLDBECK, ROSANA. n-Butanol production bySaccharomyces cerevisiaefrom protein-rich agro-industrial by-products. Brazilian Journal of Microbiology, v. 51, n. 4, SEP 2020. (19/08542-3, 16/04602-3, 15/50612-8, 15/20630-4)

OLIVEIRA, T. C. G.; INTERLANDI, M. A.; HANLON, K. E.; TORRES-MAYANGA, P. C.; SILVELLO, M. A. C.; TIMKO, M. T.; ROSTAGNO, M. A.; GOLDBECK, R.; FORSTER-CARNEIRO, T.. Integration of Subcritical Water and Enzymatic Hydrolysis to Obtain Fermentable Sugars and Second-Generation Ethanol from Sugarcane Straw. BioEnergy Research, v. 15, n. 2, p. 12-pg., 2021-04-17. (19/08542-3, 18/05999-0, 18/14582-5, 13/04304-4, 18/14938-4)

MONTEIRO, CARLA R. M.; AVILA, PATRICIA F.; PEREIRA, MARIA ANGELICA F.; PEREIRA, GABRIELA N.; BORDIGNON, SIDNEI E.; ZANELLA, EDUARDO; STAMBUK, BORIS U.; DE OLIVEIRA, DEBORA; GOLDBECK, ROSANA; POLETTO, PATRICIA. Hydrothermal treatment on depolymerization of hemicellulose of mango seed shell for the production of xylooligosaccharides. Carbohydrate Polymers, v. 253, FEB 1 2021. (19/08542-3)

MARTINS, MANOELA; SILVA, MARCOS FELLIPE; DINAMARCO, TAISA MAGNANI; GOLDBECK, ROSANA. Novel bi-functional thermostable chimeric enzyme for feasible xylo-oligosaccharides production from agro-industrial wastes. Process Biochemistry, v. 122, p. 10-pg., 2022-11-01. (20/02871-2, 19/08542-3)

SGANZERLA, WILLIAM GUSTAVO; DA SILVA, MARCOS FELLIPE; ZABOT, GIOVANI L.; GOLDBECK, ROSANA; MUSSATTO, SOLANGE I.; FORSTER-CARNEIRO, TANIA. Techno-economic assessment of subcritical water hydrolysis of brewer?s spent grains to recover xylo-oligosaccharides. JOURNAL OF SUPERCRITICAL FLUIDS, v. 196, p. 12-pg., 2023-02-26. (19/26925-7, 19/08542-3, 18/14938-4, 21/12762-9)

SILVELLO, MARIA AUGUSTA DE CARVALHO; GASPAROTTO, GIOVANNA ALVES; FERREIRA, GABRIELA F.; SANTOS, LUCIELEN OLIVEIRA; FREGOLENTE, LEONARDO V.; GOLDBECK, ROSANA. Nutrient Optimization Strategy to Increase the Carbohydrate Content of Chlorella vulgaris and Evaluation of Hydrolysis and Fermentation Performance. BioEnergy Research, v. N/A, p. 10-pg., 2023-09-06. (19/08542-3, 18/20787-9, 15/20630-4)

MARTINS, MANOELA; TRAMONTINA, ROBSON; SQUINA, FABIO MARCIO; DINAMARCO, TAISA MAGNANI; GOLDBECK, ROSANA. Synergism for xylo-oligosaccharides, p-coumaric and ferulic acid production, and thermostability modulation of GH 62 alpha-L-arabinofuranosidase. BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, v. 44, p. 11-pg., 2022-08-26. (20/02871-2, 15/50590-4, 19/08542-3)

AVILA, PATRICIA F.; DE MELLO, ALLAN H. F.; GOLDBECK, ROSANA. Cello-oligosaccharides production from multi-stage enzymatic hydrolysis by lignocellulosic biomass and evaluation of prebiotic potential. INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES, v. 85, p. 8-pg., 2023-03-21. (19/08542-3, 17/24503-2)

MARTINS, MANOELA; SGANZERLA, WILLIAM GUSTAVO; FORSTER-CARNEIRO, TANIA; GOLDBECK, ROSANA. Recent advances in xylo-oligosaccharides production and applications: A comprehensive review and bibliometric analysis. BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, v. 47, p. 16-pg., 2023-01-09. (19/26925-7, 19/08542-3, 18/14938-4, 20/02871-2)

DA SILVA, AURENICE MARIA MOTA; ALMEIDA, FLAVIA SOUZA; DA SILVA, MARCOS FELLIPE; GOLDBECK, ROSANA; SATO, ANA CARLA KAWAZOE. How do pH and temperature influence extraction yield, physicochemical, functional, and rheological characteristics of brewer spent grain protein concentrates?. FOOD AND BIOPRODUCTS PROCESSING, v. 139, p. 12-pg., 2023-05-01. (19/08542-3, 18/21987-1, 11/06083-0, 09/54137-1, 15/11984-7, 19/27354-3)

AVILA, PATRICIA F.; DE MELLO, ALLAN H. F.; FORTE, MARCUS B. S.; COSTA, FATIMA A. DE ALMEIDA; GOLDBECK, ROSANA. Fractionation of functional oligosaccharides produced from sugarcane straw using serial nanofiltration membranes and their influence on prebiotic potential. Food Research International, v. 172, p. 7-pg., 2023-06-23. (19/08542-3, 17/24503-2)

AVILA, PATRICIA F.; MARTINS, MANOELA; GOLDBECK, ROSANA. Enzymatic Production of Xylooligosaccharides from Alkali-Solubilized Arabinoxylan from Sugarcane Straw and Coffee Husk. BioEnergy Research, SEP 2020. (19/08542-3, 15/20630-4, 15/50612-8, 17/24503-2)

BARBOSA, FERNANDO CESAR; SILVELLO, MARIA AUGUSTA; GOLDBECK, ROSANA. Cellulase and oxidative enzymes: new approaches, challenges and perspectives on cellulose degradation for bioethanol production. Biotechnology Letters, v. 42, n. 6, p. 875-884, JUN 2020. (18/20787-9, 19/08542-3, 17/16159-0)

MARTINS, MANOELA; SATO, ANA CARLA KAWAZOE; OGINO, KENJI; GOLDBECK, ROSANA. valuating the addition of xylooligosaccharides into alginate-gelatin hydrogel. Food Research International, v. 147, SEP 2021. (18/14223-5, 19/08542-3, 15/20630-4)

SILVELLO, MARIA AUGUSTA DE CARVALHO; CAMARGO, ALINE FRUMI; SCAPINI, THAMARYS; PAUDEL, SHUKRA RAJ; TREICHEL, HELEN; GOLDBECK, ROSANA. Enzymatic Hydrolysis Intensification of Lignocellulolytic Enzymes Through Ultrasonic Treatment. BioEnergy Research, SEP 2021. (19/08542-3, 15/20630-4, 18/20787-9)

AVILA, PATRICIA F.; MARTINS, MANOELA; GOLDBECK, ROSANA. Enzymatic Production of Xylooligosaccharides from Alkali-Solubilized Arabinoxylan from Sugarcane Straw and Coffee Husk. BioEnergy Research, v. 14, n. 3, p. 739-751, SEP 2021. (17/24503-2, 19/08542-3, 15/50612-8, 15/20630-4)

PEREIRA, MARIA ANGELICA F.; MONTEIRO, CARLA ROANA M.; PEREIRA, GABRIELA N.; JUNIOR, SIDNEI E. BORDIGNON; ZANELLA, EDUARDO; AVILA, PATRICIA F.; STAMBUK, BORIS U.; GOLDBECK, ROSANA; DE OLIVEIRA, DEBORA; POLETTO, PATRICIA. Deconstruction of banana peel for carbohydrate fractionation. Bioprocess and Biosystems Engineering, v. 44, n. 2, p. 297-306, FEB 2021. (19/08542-3)

SAMPAIO, ULLIANA MARQUES; DA SILVA, MARCOS FELLIPE; GOLDBECK, ROSANA; CLERICI, MARIA TERESA PEDROSA SILVA. Technological and prebiotic aspects of young bamboo culm flour (Dendrocalamus latiflorus) combined with rice flour to produce healthy extruded products. Food Research International, v. 165, p. 12-pg., 2023-01-27. (19/08542-3)

MACIEL-SILVA, FRANCISCO W.; LACHOS-PEREZ, DANIEL; BULLER, LUZ SELENE; SGANZERLA, WILLIAM G.; PEREZ, MONTSERRAT; ROSTAGNO, MAURICIO A.; FORSTER-CARNEIRO, TANIA. Green Extraction Processes for Complex Samples from Vegetable Matrices Coupled with On-Line Detection System: A Critical Review. Molecules, v. 27, n. 19, p. 21-pg., 2022-10-01. (18/14582-5, 20/10323-5, 19/08542-3, 18/14938-4)

OLIVEIRA, T. C. G.; HANLON, K. E.; INTERLANDI, M. A.; TORRES-MAYANGA, P. C.; SILVELLO, M. A. C.; LACHOS-PEREZ, D.; TIMKO, M. T.; ROSTAGNO, M. A.; GOLDBECK, R.; FORSTER-CARNEIRO, T.. Subcritical water hydrolysis pretreatment of sugarcane bagasse to produce second generation ethanol. JOURNAL OF SUPERCRITICAL FLUIDS, v. 164, OCT 1 2020. (18/14582-5, 18/14938-4, 18/05999-0, 19/08542-3)

LOPES, ALBERTO MOURA MENDES; MARTINS, MANOELA; GOLDBECK, ROSANA. Heterologous Expression of Lignocellulose-Modifying Enzymes in Microorganisms: Current Status. MOLECULAR BIOTECHNOLOGY, v. 63, n. 3, p. 184-199, MAR 2021. (19/08542-3)

AVILA, PATRICIA F.; SILVA, MARCOS F.; MARTINS, MANOELA; GOLDBECK, ROSANA. Cello-oligosaccharides production from lignocellulosic biomass and their emerging prebiotic applications. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, v. 37, n. 5, MAY 2021. (19/08542-3, 15/20630-4, 15/50612-8, 17/24503-2)

MARTINS, MANOELA; DINAMARCO, TAISA MAGNANI; GOLDBECK, ROSANA. Recombinant chimeric enzymes for lignocellulosic biomass hydrolysis. Enzyme and Microbial Technology, v. 140, OCT 2020. (19/08542-3)

MARTINS, MANOELA; AVILA, PATRICIA FELIX; PAIM DE ANDRADE, CRISTIANE CONTE; GOLDBECK, ROSANA. Synergic recombinant enzyme association to optimize xylo-oligosaccharides production from agricultural waste. BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, v. 28, p. 8-pg., 2020-09-01. (15/20630-4, 18/14223-5, 15/50612-8, 19/08542-3)

MORETTI, LETICIA KORIN; RAMOS, KAZUMI KAWASAKI; AVILA, PATRICIA FELIX; GOLDBECK, ROSANA; VIEIRA, JOSELIO BATISTA; EFRAIM, PRISCILLA. Influence of cocoa varieties on carbohydrate composition and enzymatic activity of cocoa pulp. Food Research International, v. 173, p. 8-pg., 2023-09-12. (19/08542-3)

BARBOSA, FERNANDO CESAR; MARTINS, MANOELA; BRENELLI, LIVIA BEATRIZ; FERRARI, FELIPE AUGUSTO; SOARES FORTE, MARCUS BRUNO; RABELO, SARITA CANDIDA; FRANCO, TELMA TEIXEIRA; GOLDBECK, ROSANA. Screening of potential endoglucanases, hydrolysis conditions and different sugarcane straws pretreatments for cello-oligosaccharides production. Bioresource Technology, v. 316, p. 8-pg., 2020-11-01. (18/14223-5, 19/08542-3, 17/15477-8, 15/50612-8, 19/19976-4)