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Systemic impact of oral microbiome in periodontitis-affected subjects and their descendants

Grant number: 19/09740-3
Support type:Regular Research Grants
Duration: October 01, 2019 - September 30, 2021
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Renato Corrêa Viana Casarin
Grantee:Renato Corrêa Viana Casarin
Home Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
Assoc. researchers:Márcio Zaffalon Casati ; Mauro Pedrine Santamaria ; Melline Fontes Noronha

Abstract

Recent studies have shown that the descendatns of individuals affected by aggressive periodontitis, currently named Periodontitis Grade C Stage 3-4, present an oral microbiome with dysbiotic characteristics, with resilience to changes after treatment. In addition, it is suggested that the acquisition of part of this microbiota occurs early in the first years of life, when the intestinal microbiota is being established, essential for systemic health. Thus, the general objective of the present study is to evaluate the systemic impact of oral dysbiosis presented by those affected by Periodontitis (PerioC) as well as in their descendants, also evaluating the biofilm gene expression profile in these conditions. As specific objectives, we have: 1. As a specific objective to evaluate the impact of the oral microbiota on the intestinal microbiota and systemic health of affected by Perio4C and its descendants, 20 pairs of parents affected by Perio4c and children (6-12years), as well as 20 pairs of periodontally healthy parents and children (with gender and age matching), will be selected. From them oral biofilm samples (saliva and subgingival), stool and urine will be collected, as well as evaluation of general health parameters systemic. From the biofilm and stool samples, DNA extraction will be made and sequencing (MiSeq Illumina, regions V1-V3 and V4-V7 16S rRNA) for a microbiome analysis. From the urine and stool samples, intestinal mucosa integrity parameters will be assessed by determining the levels of Calprotectin and Lactoferrin (by the Luminex / MAGpix platform) in feces and Claudina-2, -3 and -4, as well as Zonulin, (by the ELISA platform) in the urine. The parameters of systemic health will be anthropometric data, history of diseases and recent medical data. Based on the pilot study findings of transcriptome differences in the biofilm of these individuals affected by PerioC, the second specific objective will be to evaluate the profile of genes differentially expressed in the biofilm of those affected by Perio4C and their offspring. Thus, oral biofilm samples will be collected around the 1st molars of the pairs (described above), packed in RNAlater and, from the extraction of RNA, cDNA preparation and by the PCR real time reaction, differentially expressed genes will have their specific expression and compared between the parents / children of each group. Data from all stages will be correlated and compared by specific tests, with a significance level of 5%. (AU)