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The use of ferritin nanoparticles as a basis for the development of antitumor vaccine delivery systems

Abstract

In the new era of subunit vaccines made from proteins, peptides or DNA antigens, there is a greater need for potent adjuvants that increase the immunogenicity. In this context, the nanotechnology and materials science appear as promising opportunities to meet this challenge(MAMO; POLAND, 2012). Ferritin nanoparticles are protein-based supramolecular complexes that have highly symmetric and uniformly sized hollow spherical architectures. They are precisely self-assembled from 24 copies of identical 20 kDa subunits which can be easily and accurately manipulated by genetic modifications and chemical bioconjugations (HAN et al., 2014). Thus, the recent discovery of the ferritin ability to encapsulate different types of molecules, having a role as nanostructured release system, has identified a new platform for use in bionanotechnology (HE; MARLES-WRIGHT, 2015). The development of this project has as main objective to contribute to the establishment of a new infrastructure and technology platform for R&D of biological nanostructured release systems for vaccine delivery. To this end, it proposes to evaluate ferritin-based delivery systems of a DNA vaccine for melanoma treatment. The development of the present proposal will have as specific objectives: I) Obtainment and characterization of the DNA vaccine encoding EGFRvIII-incorporated nanostructured delivery systems; and II) Evaluation of the vaccine formulations immunogenicity by the determination of the humoral and cellular immune responses in previously immunized animals. (AU)

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VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)