Biomarkers and tumor stem cells in the treatment of head and neck cancer

• Grant number: 18/26166-6
• Support Opportunities: Regular Research Grants
• Duration: December 01, 2019 - September 30, 2022
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Eny Maria Goloni Bertollo
• Grantee: Eny Maria Goloni Bertollo
• Host Institution: Faculdade de Medicina de São José do Rio Preto (FAMERP). Secretaria de Desenvolvimento Econômico (São Paulo - Estado). São José do Rio Preto , SP, Brazil
• Associated researchers: Erika Cristina Pavarino ; José Victor Maniglia ; Rosa Sayoko Kawasaki Oyama

Abstract

Head and neck cancer is a malignant tumor class that affects the regions of the mouth, larynx, and nasopharynx. This heterogeneity of head and neck tumors has made difficult the early diagnosis and prognosis of the disease. The analysis at the molecular level is now an indispensable tool in the fight against cancer. The identification of biomarkers can define cellular and molecular changes associated with malignant transformation and may present potential applications in oncology, including risk assessment, screening, differential diagnosis, prognosis determination, treatment response prediction and monitoring of disease progression. This project aims at the identification of biomarkers related to cancer, evaluation of chemotherapy in tumor stem cells, gene and protein expression and the evaluation of microRNAs in the development of the disease. Primary tumors and lineages of head and neck cancer will be studied and the methodology to be used includes cell culture, flow cytometry, MTS technique, cell sorting technique, transfection techniques, RNA extraction and protein from the samples, real-time PCR techniques, ELISA, and to identify and validate ligands with potential use as new inhibitors of molecular markers involved in the epithelial-mesenchymal transition process. The results will be evaluated by statistical analysis in specific programs. The data will be written in scientific articles and published in magazines of high impact in the literature, providing technological innovation, efficient biomarkers, collaborating significantly in the prognosis, diagnosis, and treatment of cancer. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CUZZIOL, CAROLINE IZAK; URBANIN CASTANHOLE-NUNES, MARCIA MARIA; PAVARINO, ERIKA CRISTINA; GOLONI-BERTOLLO, ENY MARIA. MicroRNAs as regulators of VEGFA and NFE2L2 in cancer. Gene, v. 759, OCT 30 2020. (18/26166-6)

SERAFIM JUNIOR, VILSON; DE MENDONCA FERNANDES, GLAUCIA MARIA; DE OLIVEIRA-CUCOLO, JULIANA GARCIA; PAVARINO, ERIKA CRISTINA; GOLONI-BERTOLLO, ENY MARIA. Role of Tropomyosin-related kinase B receptor and brain-derived neurotrophic factor in cancer. CYTOKINE, v. 136, DEC 2020. (18/26166-6)

FERNANDES, GLAUCIA MARIA DE MENDONCA; JUNIOR, VILSON SERAFIM; GALBIATTI-DIAS, ANA LIVIA SILVA; FERREIRA, LETICIA ANTUNES MUNIZ; CASTANHOLE-NUNES, MARCIA MARIA URBANIN; KAWASAKI-OYAMA, ROSA SAYOKO; MANIGLIA, JOSE VICTOR; PAVARINO, ERIKA CRISTINA; GOLONI-BERTOLLO, ENY MARIA. Original Treatment effects of the EGFR pathway drugs on head and neck cancer stem cells. AMERICAN JOURNAL OF CANCER RESEARCH, v. 12, n. 9, p. 16-pg., 2022-01-01. (15/04403-8, 18/26166-6)

BEZERRA, M. A. S.; FERREIRA, L. A. M.; KAWASAKI-OYAMA, R. S.; CUZZIOL, C. I.; NASCIMENTO, M. C. A.; CASTANHOLE-NUNES, M. M. U.; PAVARINO, E. C.; MANIGLIA, J. V.; GOLONI-BERTOLLO, E. M.. Hypoxia-induced c(a)over-capncer stem cell accumulation in primary cell culture of head and neck squamous cell carcinoma. European Journal of Human Genetics, v. 28, n. SUPPL 1, p. 2-pg., 2020-12-01. (16/20087-1, 18/26166-6)

CUZZIOL, CAROLINE IZAK; MARZOCHI, LUDIMILA LEITE; POSSEBON, VITORIA SCAVACINI; KAWASAKI-OYAMA, ROSA SAYOKO; MATTOS, MARLON FRAGA; SERAFIM JUNIOR, VILSON; MUNIZ FERREIRA, LETICIA ANTUNES; PAVARINO, ERIKA CRISTINA; URBANIN CASTANHOLE-NUNES, MARCIA MARIA; GOLONI-BERTOLLO, ENY MARIA. Regulation of VEGFA, KRAS, and NFE2L2 Oncogenes by MicroRNAs in Head and Neck Cancer. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 23, n. 13, p. 12-pg., 2022-07-01. (20/03209-1, 15/04403-8, 18/26166-6)