Screening of the amino acid hypusine molecular actions

• Grant number: 19/06951-3
• Support Opportunities: Regular Research Grants
• Duration: March 01, 2020 - February 28, 2023
• Field of knowledge: Biological Sciences - Biology
• Principal Investigator: Augusto Ducati Luchessi
• Grantee: Augusto Ducati Luchessi
• Host Institution: Faculdade de Ciências Aplicadas (FCA). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil
• Associated researchers: Karina Danielle Pereira ; Leticia Tamborlin

Abstract

The amino acid hypusine was first identified in 1971 from bovine brain extract. Later, this amino acid was found in liver, kidney, muscle and blood of other mammals. In addition, hypusine is a substrate to synthesise the ±-(³-aminobutyryl)-hypusine (GABA-hypusine) dipeptide. The GABA is the most representative inhibitory neurotransmitter of the central nervous system. It is noted that the dipeptide GABA-hypusine has partial structural relationship with the dipeptide GABA-histidine (homocarnosine). Homocarnosine is widely studied and presents antioxidant, neurotransmitter and metal chelating activities, being considered an important modulator of neuronal excitability. The hypusine amino acid is formed from a post-translational modification that occurs at the eukaryotic translation initiation factor 5A (eIF5A) and the free form is derived from the degradation of this protein. The eIF5A protein is the only one described as having the amino acid residue hypusine, which is essential for its activity. In this context, although hypusine and GABA-hypusine were discovered in the 70's and 80's, respectively, their biological activities are still unknown. The present project aims to characterize the distribution of hypusine and GABA-hypusine in different tissues of mice and to evaluate the cellular effects of the amino acid hypusine in cerebral cells, aiming at the identification of potential biotechnological properties. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TAMBORLIN, LETICIA; SUMERE, BEATRIZ ROCCHETTI; DE SOUZA, MARIANA CORREA; PESTANA, NATHALIE FORTES; AGUIAR, ANA CAROLINA; EBERLIN, MARCOS NOGUEIRA; SIMABUCO, FERNANDO MOREIRA; ROSTAGNO, MAURICIO ARIEL; LUCHESSI, AUGUSTO DUCATI. Characterization of pomegranate peel extracts obtained using different solvents and their effects on cell cycle and apoptosis in leukemia cells. FOOD SCIENCE & NUTRITION, v. 8, n. 10, AUG 2020. (18/17089-8, 15/18119-0, 19/06951-3, 10/18095-0, 18/14818-9, 17/21914-1, 13/04304-4)

PEREIRA, KARINA D.; TAMBORLIN, LETICIA; DE LIMA, I, TANES; CONSONNI, SILVIO R.; SILVEIRA, LEONARDO R.; LUCHESSI, AUGUSTO D.. Alternative human eIF5A protein isoform plays a critical role in mitochondria. Journal of Cellular Biochemistry, v. 122, n. 5, JAN 2021. (17/21914-1, 13/23620-4, 19/06951-3, 10/18095-0)

TAIPINA, MARCIA O.; DE MELLO, MARIANA G.; TAMBORLIN, LETICIA; PEREIRA, KARINA D.; LUCHESSI, AUGUSTO D.; CREMASCO, ALESSANDRA; CARAM, RUBENS. A novel Ag doping Ti alloys route: Formation and antibacterial effect of the TiO2 nanotubes. Materials Chemistry and Physics, v. 261, MAR 1 2021. (19/06951-3, 16/24693-3, 17/21914-1, 14/00159-2)