Advanced search
Start date
Betweenand

Genomic, transcriptomic and phenotypic characterization of Campylobacter jejuni strains isolated from diverse source during 20 years in Brazil

Abstract

Campylobacteriosis is a zoonosis with a large distribution worldwide, and Campylobacter jejuni has been an important cause of gastroenteritis in humans in many European countries, as well as in the United States, Canada, Australia, among others. However, in Brazil the study and isolation of C. jejuni are less frequently reported, which makes it difficult to evaluate the importance and dimension of this pathogen as a cause of disease in humans in our country. The aims of this project are to analyze and to compare, through whole genome sequencing, transcriptome sequencing and virulence-related phenotypic tests, C. jejuni strains isolated from humans, animals, the environment and food during 20 years in Brazil. The comparative analysis of the whole genome sequencing will be performed in 116 C. jejuni strains isolated from humans (47), animals (35), food (32) and the environment (02). The phenotypic tests of tolerance of temperature variations, acid stress and oxidative stress, virulence in Galleria mellonella, assay in vivo using the chick embryo chorioallantoic membrane, invasion of CACO-2 cells and multiplication and survival in U937 human macrophages will be performed in 46 C. jejuni strains isolated from humans (18), animals (14) and food (14). Furthermore, the transcriptome analysis of six C. jejuni strains that present significant differences against the phenotypic tests of acid and oxidative stress will be performed, and the RNAs will be extracted after submitting the strains to each stress and also in ideal conditions of growth. Due to the paucity of studies involving C. jejuni strains in our country, studies that aim to analyse the whole genome sequencing and to elucidate the possible mechanisms of pathogenicity and virulence in such strains are innovative and extremely important. Therefore, the results to be obtained should contribute for a better characterization of C. jejuni strains isolated during 20 years in Brazil, collaborating to the understanding of this pathogen of great importance in the world. (AU)

Articles published in Agência FAPESP Newsletter about the research grant:
Articles published in other media outlets (0 total):
More itemsLess items
VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)

Scientific publications (8)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SERIBELLI, AMANDA AP.; DA SILVA, PATRICK; DA CRUZ, MARCELO FERREIRA; DE ALMEIDA, FERNANDA; FRAZAO, MILIANE R.; MEDEIROS, MARTA I. C.; RODRIGUES, DALIA DOS P.; KICH, JALUSA D.; DE JESUS BENEVIDES, LEANDRO; SOARES, SIOMAR DE C.; et al. nsights about the epidemiology of Salmonella Typhimurium isolates from different sources in Brazil using comparative genomic. GUT PATHOGENS, v. 13, n. 1, . (17/06633-6, 16/24716-3, 19/19338-8)
VILELA, FELIPE PINHEIRO; RODRIGUES, DALIA DOS PRAZERES; ALLARD, MARC WILLIAM; FALCAO, JULIANA PFRIMER. Genomic characterization and antimicrobial resistance profiles of Salmonella enterica serovar Infantis isolated from food, humans and veterinary-related sources in Brazil. Journal of Applied Microbiology, . (19/19338-8, 19/06947-6)
VILELA, FELIPE PINHEIRO; PRIBUL, BRUNO ROCHA; RODRIGUES, DALIA DOS PRAZERES; BALKEY, MARIA; ALLARD, MARC; FALCAO, JULIANA PFRIMER. Draft Genome Sequences of 80 Salmonella enterica Serovar Infantis Strains Isolated from Food, Environmental, Human, and Veterinary Sources in Brazil. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 10, n. 24, . (19/06947-6, 19/19338-8)
VILELA, FELIPE PINHEIRO; FALCAO, JULIANA PFRIMER. Analysis of the antimicrobial resistance gene frequency in whole-genome sequenced Vibrio from Latin American countries. Journal of Medical Microbiology, v. 70, n. 9, . (19/06947-6, 19/19338-8)
GOMES, CAROLINA NOGUEIRA; BARKER, DILLON OLIVER REESE; DUQUE, SHEILA DA SILVA; CHE, EMILY VICTORIA; JAYAMANNA, VASENA; TABOADA, EDUARDO NAPOLEON; FALCAO, JULIANA PFRIMER. Campylobacter coli isolated in Brazil typed by core genome Multilocus Sequence Typing shows high genomic diversity in a global context. INFECTION GENETICS AND EVOLUTION, v. 95, . (18/26043-1, 16/24716-3, 15/23408-0, 19/19338-8)
FRAZAO, MILIANE RODRIGUES; CAO, GUOJIE; MEDEIROS, MARTA INES CAZENTINI; DUQUE, SHEILA DA SILVA; ALLARD, MARC WILLIAM; FALCAO, JULIANA PFRIMER. Antimicrobial Resistance Profiles and Phylogenetic Analysis ofCampylobacter jejuniStrains Isolated in Brazil by Whole Genome Sequencing. MICROBIAL DRUG RESISTANCE, v. 27, n. 5, p. 660-669, . (18/06904-2, 14/13029-0, 16/24716-3, 19/19338-8)
SERIBELLI, AMANDA APARECIDA; MACHADO RIBEIRO, TAMARA R.; DA SILVA, PATRICK; MARTINS, ISABELA MANCINI; VILELA, FELIPE PINHEIRO; CAZENTINI MEDEIROS, I, MARTA; PERONNI, KAMILA CHAGAS; DA SILVA JUNIOR, WILSON ARAUJO; MOREIRA, CRISTIANO GALLINA; FALCAO, JULIANA PFRIMER. Salmonella Typhimurium ST313 isolated in Brazil revealed to be more invasive and inflammatory in murine colon compared to ST19 strains. JOURNAL OF MICROBIOLOGY, v. 59, n. 9, . (17/06633-6, 16/12744-2, 19/19338-8, 19/03049-7, 16/24716-3)
SERIBELLI, AMANDA APARECIDA; DA SILVA, PATRICK; FRAZAO, MILIANE RODRIGUES; KICH, JALUSA DEON; ALLARD, MARC W.; FALCAO, JULIANA PFRIMER. Phylogenetic relationship and genomic characterization of Salmonella Typhimurium strains isolated from swine in Brazil. INFECTION GENETICS AND EVOLUTION, v. 93, . (16/24716-3, 17/06633-6, 19/19338-8)

Please report errors in scientific publications list by writing to: cdi@fapesp.br.