Investigating the evolution of satellite DNAs in holocentric chromosomes of pest species

• Grant number: 19/19069-7
• Support Opportunities: Regular Research Grants
• Duration: May 01, 2020 - May 31, 2022
• Field of knowledge: Biological Sciences - Genetics - Animal Genetics
• Principal Investigator: Diogo Cavalcanti Cabral de Mello
• Grantee: Diogo Cavalcanti Cabral de Mello
• Host Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil

Abstract

The satellite DNAs (satDNA) are repetitive sequences organized in tandem, forming in general large arrays and comprising large portion of eukaryote genomes. They are peculiarly enriched in heterochromatic regions, mainly in centromeres. These sequences play role in chromosomal structure and recently functionality for them has been described. The genomic and chromosomal organization and the evolutionary patterns for this DNA class are well known in species with monocentric chromosomes (located centromeres). The theories about satDNA evolution, like the library hypothesis and concerted evolution were postulated in monocentric chromosomes. On the other hand, among species with holocentric chromosomes (diffuse centromere) almost no knowledge about molecular evolution of this DNA class was obtained. This group is interesting for analysis due to the disperse organization for heterochromatin that could influence the evolution of satDNAs. The study of satDNA in species with holocentric chromosomes will allow the test of hypothesis about satDNA evolution and their impact in the karyotypic organization and variability. Moreover, it will be possible the generation of broader theories about satDNA biology. In this way, in this proposal we intend to advance in the knowledge about structure and evolution of satDNAs, using as experimental models pest insects (Hemiptera and Lepidoptera) with holocentric chromosomes. Finally, we will contribute with the genome assembling of pest species that could have applicability in the future. For this aim we will use an integrative analysis combining cytogenetics, genomics (next generation sequencing) and bioinformatics. (AU)

Scientific publications

(12)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARTI, EMILIANO; MILANI, DIOGO; BARDELLA, VANESSA B.; ALBUQUERQUE, LUCAS; SONG, HOJUN; PALACIOS-GIMENEZ, OCTAVIO M.; CABRAL-DE-MELLO, DIOGO C.. Cytogenomic analysis unveils mixed molecular evolution and recurrent chromosomal rearrangements shaping the multigene families on Schistocerca grasshopper genomes. Evolution, JUL 2021. (14/11763-8, 19/19069-7)

GASPAROTTO, ANA E.; MILANI, DIOGO; MARTI, EMILIANO; FERRETTI, ANA BEATRIZ S. M.; BARDELLA, VANESSA B.; HICKMANN, FREDERICO; ZRZAVA, MAGDA; MAREC, FRANTISEK; CABRAL-DE-MELLO, DIOGO C.. A step forward in the genome characterization of the sugarcane borer, Diatraea saccharalis: karyotype analysis, sex chromosome system and repetitive DNAs through a cytogenomic approach. CHROMOSOMA, v. 131, n. 4, p. 15-pg., 2022-10-11. (21/05908-7, 20/06188-5, 19/19069-7, 18/21772-5, 18/20351-6)

BARDELLA, VANESSA BELLINI; MILANI, DIOGO; CABRAL-DE-MELLO, DIOGO CAVALCANTI. Analysis ofHolhymenia histriogenome provides insight into the satDNA evolution in an insect with holocentric chromosomes. Chromosome Research, v. 28, n. 3-4, p. 369-380, DEC 2020. (19/19069-7, 18/21772-5)

MARTI, EMILIANO; MILANI, DIOGO; BARDELLA, VANESSA B.; ALBUQUERQUE, LUCAS; SONG, HOJUN; PALACIOS-GIMENEZ, OCTAVIO M.; CABRAL-DE-MELLO, DIOGO C.. Cytogenomic analysis unveils mixed molecular evolution and recurrent chromosomal rearrangements shaping the multigene families on Schistocerca grasshopper genomes. Evolution, v. 75, n. 8, p. 2027-2041, AUG 2021. (14/11763-8, 19/19069-7)

CABRAL-DE-MELLO, DIOGO CAVALCANTI; MAREC, FRANTISEK. Universal fluorescence in situ hybridization (FISH) protocol for mapping repetitive DNAs in insects and other arthropods. Molecular Genetics and Genomics, v. 296, n. 3, FEB 2021. (19/19069-7)

MENEZES, RODOLPHO S. T.; CABRAL-DE-MELLO, DIOGO C.; MILANI, DIOGO; BARDELLA, VANESSA B.; ALMEIDA, EDUARDO A. B.. The relevance of chromosome fissions for major ribosomal DNA dispersion in hymenopteran insects. JOURNAL OF EVOLUTIONARY BIOLOGY, v. 34, n. 9, AUG 2021. (16/21098-7, 19/19069-7, 15/02432-0, 18/09666-5, 14/11763-8)

VICARI, MARCELO RICARDO; BRUSCHI, DANIEL PACHECO; CAVALCANTI CABRAL-DE-MELLO, DIOGO; NOGAROTO, VIVIANE. Telomere organization and the interstitial telomeric sites involvement in insects and vertebrates chromosome evolution. GENETICS AND MOLECULAR BIOLOGY, v. 45, n. 3, p. 22-pg., 2022-01-01. (19/19069-7, 17/24695-9)

HICKMANN, FREDERICO; CORREA, ALBERTO SOARES; BARDELLA, VANESSA B.; MILANI, DIOGO; CLARINDO, WELLINGTON R.; SOARES, FERNANDA A. F.; CARVALHO, RENATA F.; MONDIN, MATEUS; CABRAL-DE-MELLO, DIOGO C.. Cytogenomic characterization of Euschistus (Heteroptera: Pentatomidae) species and strains reveals low chromosomal and repetitive DNAs divergences. Biological Journal of the Linnean Society, v. N/A, p. 18-pg., 2023-09-01. (18/21772-5, 19/19069-7, 18/20351-6, 21/05908-7)

CABRAL-DE-MELLO, DIOGO C.; ZRZAVA, MAGDA; KUBICKOVA, SVATAVA; RENDON, PEDRO; MAREC, FRANTISEK. The Role of Satellite DNAs in Genome Architecture and Sex Chromosome Evolution in Crambidae Moths. FRONTIERS IN GENETICS, v. 12, MAR 30 2021. (19/19069-7)

PEREIRA, JAQUELINE A.; MILANI, DIOGO; FERRETTI, ANA BEATRIZ S. M.; BARDELLA, VANESSA B.; CABRAL-DE-MELLO, DIOGO C.; LOPES, DENILCE M.. The extensive amplification of heterochromatin in Melipona bees revealed by high throughput genomic and chromosomal analysis. CHROMOSOMA, v. 130, n. 4, NOV 2021. (20/06188-5, 19/19069-7, 18/21772-5)

CABRAL-DE-MELLO, DIOGO C.; MORA, PABLO; RICO-PORRAS, JOSE M.; FERRETTI, ANA B. S. M.; PALOMEQUE, TERESA; LORITE, PEDRO. The spread of satellite DNAs in euchromatin and insights into the multiple sex chromosome evolution in Hemiptera revealed by repeatome analysis of the bug Oxycarenus hyalinipennis. Insect Molecular Biology, v. N/A, p. 13-pg., 2023-08-24. (19/19069-7)

CASTILLO, ELIO RODRIGO D.; CELESTE SCATTOLINI, M.; PALACIOS-GIMENEZ, OCTAVIO M.; MARTI, DARDO A.; CABRAL-DE-MELLO, DIOGO C.; CIGLIANO, MARIA M.. Karyotype evolution in Ronderosia grasshoppers (Orthoptera: Acrididae). ZOOLOGICAL JOURNAL OF THE LINNEAN SOCIETY, v. 198, n. 2, p. 17-pg., 2023-02-16. (19/19069-7)