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Use of ozone gas as an angiogenic agent in the equine endometrium

Grant number: 19/22728-2
Support type:Regular Research Grants
Duration: July 01, 2020 - June 30, 2022
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Reproduction
Principal Investigator:Jair Camargo Ferreira
Grantee:Jair Camargo Ferreira
Home Institution: Pró-Reitoria Adjunta de Pesquisa e Pós-Graduação. Universidade de Franca (UNIFRAN). Franca , SP, Brazil
Assoc. researchers:Jean Guilherme Fernandes Joaquim ; Marcela Aldrovani Rodrigues ; Raquel Alves dos Santos


The present study will describe for the first time the effect of ozone gas on the vascularity and ultrastructure of the equine endometrium using optical and scanning electron microscopy. In addition, potential changes in the modulation of endometrial angiogenesis will be characterized by RT-PCR. Initially, 21 females adult mares will be assigned into one of three groups according to the treatment (n = 7 mares/group): O3, ½O3, and Control groups. Mares from O3 and ½O3 groups will receive intrauterine treatment with an ozone-oxygen gas mixture containing 42¼g and 21¼g O3 mL-1, respectively. The uterus of mares from the Control group will be insufflated with oxygen (0 ¼g O3 mL-1). A last group of old mares (e18 years of age, n=7 mares) will be treated with 42¼g or 21¼g O3 mL-1, according to the initial resuts. The treatments will be performed every three days for six days (D0, D3, and D6). In each treatment, a flow of 0.25 mL gas min-1 will be infused for three consecutive minutes. Endometrial fragments will be collected immediately before the first treatment and 24 hours after the third insufflation by uterine biopsy. Posteriorly, histopathological and ultrastructural evaluation of the endometrium will be performed, besides the mRNA quantification of uterine angiogenic growth factors. Our findings will be of great value in finding alternative therapies for the treatment of infertility in mares. (AU)