DNA mettilation and X chromosome inactivation in women with Polycystic Ovary Syndrome submitted to different physical training protocols

• Grant number: 19/17618-3
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2020
• End date: November 30, 2022
• Field of knowledge: Health Sciences - Medicine - Maternal and Child Health
• Principal Investigator: Rosana Maria dos Reis
• Grantee: Rosana Maria dos Reis
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated researchers: Cristiana Libardi Miranda Furtado ; Gislaine Satyko Kogure

Abstract

The Polycystic Ovary Syndrome (PCOS) is the most common endocrinopathy in reproductive-age women, characterized by reproductive dysfunction, metabolic and psychological disorders. Genetic predisposition, environmental and lifestyle factors contribute to its pathogenesis poorly understood. Emerging evidence suggests that epigenetic modifications are related to PCOS development due to the phenotypic plasticity in its pathophysiology. DNA methylation is an important epigenetic mechanism that modulates gene expression without changes in DNA sequence that controls genomic imprinting and X Chromosome Inactivation (XCI). Epigenetic mechanisms are influenced by environmental stimulus and they are potentially reversible and sensitive to treatment. Knowing that physical exercise is a primary treatment for the management of PCOS and its complications and that there are notes that physical activity influences DNA methylation, the purpose of this study is to investigate the Global DNA methylation pattern and ICX in the syndrome before and after the intervention exercise. For these three training protocols will be analyzed and compared: progressive resistance training and continuous and intermittent aerobic training. This is a secondary data analysis of 120 volunteers with 4 parallel groups of women with PCOS: Group I - progressive resistance training (n = 30); Group II - continuous aerobic physical training (n = 30); Group III - intermittent aerobic physical training (n = 30); Group IV- without physical training (n = 30). Global DNA methylation will be evaluated using the 850K Infinium Methylation EPIC BeadChip; and ICX by the Flourescent Quantitative PCR methodology. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RIBEIRO, VICTOR BARBOSA; CHIELLI PEDROSO, DAIANA CRISTINA; KOGURE, GISLAINE SATYKO; LOPES, IRIS PALMA; SANTANA, BARBARA APARECIDA; DUTRA DE SOUZA, HUGO CELSO; FERRIANI, RUI ALBERTO; CALADO, RODRIGO TOCANTINS; MIRANDA FURTADO, CRISTIANA LIBARDI; DOS REIS, ROSANA MARIA. Short-Term Aerobic Exercise Did Not Change Telomere Length While It Reduced Testosterone Levels and Obesity Indexes in PCOS: A Randomized Controlled Clinical Trial Study. INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH, v. 18, n. 21, NOV 2021. (15/14031-0, 12/11069-9, 19/17618-3)