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Metabolomics applied to the typification and adulteration of floral honeys produced in the Santa Catarina and São Paulo States


Honey, milk, and olive oil are among the most adulterated foods worldwide. Usually, honey adulteration results from the addition of sucrose or by supplementing bee diets with other types of sugars. In connection with the honey bee production private sector of the SC e SP states, this projects aims at to chemically profile floral honeys from representative geographic production regions over the spring and summer 2020-2021. The experimental strategy herein described takes into account a complementary approach of the analytical techniques proposed, allowing identifying a series of metabolites associated to i. adulteration (i.e., IRMS), ii. biological effects (HPLC), and iii. typification (RMN e NIR) of honey samples, as well as building robust and reliable classification models. The project is a joint-venture one, gathering three institutions with recognized experience in the investigation field, as follows: Federal University of Santa Catarina/Plant Morphogenesis and Biochemistry Laboratory, São Paulo State University - Botucatu/Plant Biochemistry Laboratory, and Brazilian Biosciences National Laboratory (LNBio/CNPEM, Campinas). The team of researches joins skills on biochemistry, metabolomics, and bioinformatics (UFSC), biochemistry and plant physiology (UNESP-Botucatu), and RMN (LNBio), giving rise to a necessary and enough interdisciplinary scenario for the accomplishment of the project's goals. The set of data, information, and knowledge afforded will allow characterizing chemically complex matrices, such as honey, and identifying typical patterns of chemical composition associated to the seasonality and geographic regions of production. Besides, from the metabolomics dataset classification models will be built enabling to detect eventual adulterants and biochemical markers in samples. The results will support the typification of honeys originated from SC and SP States, a continuous demand of the producing companies to the detection of adulterated honeys, both in the internal and abroad markets. (AU)