Metabolomics applied to the typification and adulteration of floral honeys produced in the Santa Catarina and São Paulo States

• Grant number: 19/27227-1
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2020
• End date: September 30, 2023
• Field of knowledge: Agronomical Sciences - Agronomy - Crop Science
• Agreement: CONFAP - National Council of State Research Support Foundations
• Principal Investigator: Giuseppina Pace Pereira Lima
• Grantee: Giuseppina Pace Pereira Lima
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated researchers: Cristine Vanz Borges ; Maurício Luís Sforça ; Ricardo de Oliveira Orsi ; Silvana Aparecida Rocco ; Vladimir Eliodoro Costa
• Associated scholarship(s): 21/01905-3 - Metabolomics applied to the typification and adulteration of floral honeys produced in the Santa Catarina and São Paulo States, BP.TT

Abstract

Honey, milk, and olive oil are among the most adulterated foods worldwide. Usually, honey adulteration results from the addition of sucrose or by supplementing bee diets with other types of sugars. In connection with the honey bee production private sector of the SC e SP states, this projects aims at to chemically profile floral honeys from representative geographic production regions over the spring and summer 2020-2021. The experimental strategy herein described takes into account a complementary approach of the analytical techniques proposed, allowing identifying a series of metabolites associated to i. adulteration (i.e., IRMS), ii. biological effects (HPLC), and iii. typification (RMN e NIR) of honey samples, as well as building robust and reliable classification models. The project is a joint-venture one, gathering three institutions with recognized experience in the investigation field, as follows: Federal University of Santa Catarina/Plant Morphogenesis and Biochemistry Laboratory, São Paulo State University - Botucatu/Plant Biochemistry Laboratory, and Brazilian Biosciences National Laboratory (LNBio/CNPEM, Campinas). The team of researches joins skills on biochemistry, metabolomics, and bioinformatics (UFSC), biochemistry and plant physiology (UNESP-Botucatu), and RMN (LNBio), giving rise to a necessary and enough interdisciplinary scenario for the accomplishment of the project's goals. The set of data, information, and knowledge afforded will allow characterizing chemically complex matrices, such as honey, and identifying typical patterns of chemical composition associated to the seasonality and geographic regions of production. Besides, from the metabolomics dataset classification models will be built enabling to detect eventual adulterants and biochemical markers in samples. The results will support the typification of honeys originated from SC and SP States, a continuous demand of the producing companies to the detection of adulterated honeys, both in the internal and abroad markets. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NUNES, ALINE; AZEVEDO, GADIEL ZILTO; SANTOS, BEATRIZ ROCHA DOS; BORGES, CRISTINE VANZ; LIMA, GIUSEPPINA PACE PEREIRA; CROCOLI, LUANA CONTE; MOURA, SIDNEI; MARASCHIN, MARCELO. Characterization of Brazilian floral honey produced in the states of Santa Catarina and Sao Paulo through ultraviolet-visible (UV-vis), near-infrared (NIR), and nuclear magnetic resonance (NMR) spectroscopy. Food Research International, v. 162, p. 12-pg., 2022-10-01. (16/22665-2, 19/27227-1)

BASILIO, LETICIA SILVA PEREIRA; BORGES, CRISTINE VANZ; MINATEL, IGOR OTAVIO; VARGAS, PABLO FORLAN; TECCHIO, MARCO ANTONIO; VIANCELLO, FABIO; LIMA, GIUSEPPINA PACE PEREIRA. New beverage based on grapes and purple-fleshed sweet potatoes: Use of non-standard tubers. FOOD BIOSCIENCE, v. 47, p. 10-pg., 2022-06-01. (19/27227-1, 16/22665-2)

RODRIGUES DA SILVA, MARLON JOCIMAR; MAIA PAIVA, ANA PAULA; DE SOUZA, JOYCE FAGUNDES; DA SILVA PADILHA, CARLA VALERIA; PEREIRA BASILIO, LETICIA SILVA; LIMA, MARCOS DOS SANTOS; PEREIRA, GIULIANO ELIAS; CORREA, LUIZ CLAUDIO; VIANELLO, FABIO; PEREIRA LIMA, GIUSEPPINA PACE; et al. Phytochemical profile of Brazilian grapes (Vitis labrusca and hybrids) grown on different rootstocks. PLoS One, v. 17, n. 10, p. 15-pg., 2022-10-20. (15/16440-5, 19/27227-1, 16/22665-2)

DOMINGUES NETO, FRANCISCO JOSE; BORGES, CRISTINE VANZ; PEREIRA LIMA, GIUSEPPINA PACE; PIMENTEL JUNIOR, ADILSON; MONTEIRO, GEAN CHARLES; FIGUEIRA, RICARDO; VENTURINI FILHO, WALDEMAR GASTONI; MINATEL, IGOR OTAVIO; MOURA, MARA FERNANDES; TECCHIO, MARCO ANTONIO. Improvement of biogenic amines in grape juice from Vitis labrusca and hybrid grapes grown in different training systems. JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY, v. 98, n. 2, p. 10-pg., 2022-09-16. (15/16440-5, 19/27227-1, 16/07510-2, 16/22665-2)

LIMA, GIUSEPPINA PACE PEREIRA; COSTA, VLADMIR ELIODORO; NUNES, ALINE; BASILIO, LETICIA SILVA PEREIRA; BORGES, CRISTINE VANZ; MONTEIRO, GEAN CHARLES; MINATEL, IGOR OTAVIO; DENADAI, JULIANA CELIA; ORSI, RICARDO; VIANELLO, FABIO; et al. Biogenic amines and stable isotopes in the quality and authenticity of honeys from Brazil. Food Chemistry, v. 427, p. 13-pg., 2023-06-30. (16/22665-2, 19/27227-1)

BORGES, CRISTINE VANZ; NUNES, ALINE; COSTA, VLADIMIR ELIODORO; ORSI, RICARDO DE OLIVEIRA; PEREIRA BASILIO, LETICIA SILVA; MONTEIRO, GEAN CHARLES; MARASCHIN, MARCELO; PEREIRA LIMA, GIUSEPPINA PACE. Tryptophan and Biogenic Amines in the Differentiation and Quality of Honey. INTERNATIONAL JOURNAL OF TRYPTOPHAN RESEARCH, v. 15, p. 12-pg., 2022-05-01. (16/22665-2, 19/27227-1)