Rheological analysis of bioactive gel based on silk fibroin/PLDLA/simvastatin for 3D printing

• Grant number: 20/09917-8
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2020
• End date: May 31, 2023
• Field of knowledge: Engineering - Biomedical Engineering - Medical Engineering
• Principal Investigator: Eliana Aparecida de Rezende Duek
• Grantee: Eliana Aparecida de Rezende Duek
• Host Institution: Faculdade de Ciências Médicas e da Saúde (FCMS). Pontifícia Universidade Católica de São Paulo (PUC-SP). Sorocaba , SP, Brazil
• Associated researchers: Cedric Dicko ; Daniel Komatsu ; Moema de Alencar Hausen

Abstract

In the last few decades, Bone Tissue Engineering (BTE) has been developed as an effective and alternative approach for treating large bone defects. Three-dimensional (3D) bioprinting is an emerging technology expected to revolutionize the field of tissue engineering and regenerative medicine. Several efforts have been made to stimulate bone regeneration by the release of osteogenic small molecules such as Simvastatin, because it increases osteogenesis. However, its immobilization in a scaffold/drug system is still an open challenge. Silk fibroin (SF) has been proven to be a promising biomaterial for scaffold fabrication in general and its remarkable mechanical properties predestine it for bone TE applications controllable biodegradability, and suitable cell adhesion/proliferation. Therefore, the control of the viscosity of the FS solution would guarantee the improvement in the quality of the printed structure and reproducibility. The aim of this work is to study the rheological properties of gel based on FS and PLDLA, with SIN, in order to obtain the best composition for 3D printing of scaffold that will be used in bone tissue regeneration. It is important to note that there are no reports in the scientific literature of this gel proposed in this project. FS-PLDLA-SIN solutions will be prepared in formic acid, changing the concentrations of SF, PLDLA and SIM, using a factorial experiment. Thus, the following analysis will be carried out in this project: rheological study of these different compositions, 3D printing of the better gel composition, FTIR, X-ray Diffraction, thermal analysis, simvastatin releasing test, degradation analysis, analysis of osteoblasts cells culture by laser confocal microscopy. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

QUEVEDO, BRUNA, V; KOMATSU, DANIEL; REZENDE, MAIRA DE LOURDES; DUEK, ELIANA APARECIDA DE REZENDE. Synthesis of epoxidized natural rubber grafted with hyaluronic acid for the development of biomaterials. International Journal of Biological Macromolecules, v. 244, p. 9-pg., 2023-06-15. (20/09917-8)

PEDRINI, FLAVIA; GOMES, RODRIGO CESAR; MORAES, ARIANA SOUZA; ANTUNES, BIANCA SABINO LEOCADIO; MOTTA, ADRIANA CRISTINA; DAVILA, JOSE LUIS; HAUSEN, MOEMA ALENCAR; KOMATSU, DANIEL; DUEK, ELIANA APARECIDA REZENDE. Poly(L-co-D,L-lactic acid-co-trimethylene carbonate) for extrusion-based 3D printing: Comprehensive characterization and cytocompatibility assessment. Polymer, v. 290, p. 12-pg., 2023-12-20. (20/09917-8)

ASAMI, JESSICA; QUEVEDO, BRUNA, V; SANTOS JR, ARNALDO R.; GIORNO, LUCIANA PASTENA; KOMATSU, DANIEL; DUEK, ELIANA APARECIDA DE REZENDE. The impact of non-deproteinization on physicochemical and biological properties of natural rubber latex for biomedical applications. International Journal of Biological Macromolecules, v. 253, p. 12-pg., 2023-09-12. (20/09917-8)

DE SOUZA, WINICIUS RUIS; QUEVEDO, BRUNA V.; GARCIA, PAMELA SOTO; DA SILVA, LUCAS CAMARGO SOARES CARVALHO; KOMATSU, DANIEL; DUEK, ELIANA APARECIDA DE REZENDE. Synthesis and physical-chemical characterization of chitosan grafted with hyaluronic acid as potential application in biomaterials. POLYMER BULLETIN, v. N/A, p. 19-pg., 2024-12-04. (23/17083-8, 20/09917-8)

KOMATSU, DANIEL; CABRERA, ANDREA RODRIGUES ESPOSITO; QUEVEDO, BRUNA VANESSA; ASAMI, JESSICA; MOTTA, ADRIANA CRISTINA; DE MORAES, STEPHEN CHRISTINA; DUARTE, MARCIA ADRIANA TOMAZ; HAUSEN, MOEMA DE ALENCAR; DUEK, ELIANA APARECIDA DE REZENDE. Meniscal repair with additive manufacture of bioresorbable polymer: From physicochemical characterization to implantation of 3D printed poly (L-co-D, L lactide-co-trimethylene carbonate) with autologous stem cells in rabbits. JOURNAL OF BIOMATERIALS APPLICATIONS, v. 39, n. 1, p. 14-pg., 2024-04-22. (10/00318-2, 20/09917-8)

BEVILAQUA, VANESSA R.; PELENTIR, GABRIEL F.; HAUSEN, MOEMA A.; DUEK, ELIANA A. R.; VIVIANI, VADIM R.. Selection and Engineering of Novel Brighter Bioluminescent Reporter Gene and Color- Tuning Luciferase for pH-Sensing in Mammalian Cells. BIOSENSORS-BASEL, v. 15, n. 1, p. 13-pg., 2025-01-01. (22/03538-0, 22/04800-0, 17/22262-8, 20/09917-8, 20/07649-6)