Genomics and trascriptome of virus-vector-host plant relationship in the pathosytem of Brevipalpus transmitted viruses; systematic and evolution of Brevipalpus mites and their endosymbionts; new strategies to manage citrus Leprosis in the S. Paulo State

• Grant number: 19/25078-9
• Support Opportunities: Research Projects - Thematic Grants
• Start date: February 01, 2021
• End date: January 31, 2026
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Principal Investigator: Elliot Watanabe Kitajima
• Grantee: Elliot Watanabe Kitajima
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Pesquisadores principais: Juliana de Freitas Astúa
• Associated researchers: Aline Daniele Tassi ; Celso Omoto ; Daniel Júnior de Andrade ; Denise Navia Magalhães Ferreira ; Gilberto José de Moraes ; João Paulo Rodrigues Marques ; Jorge Alberto Marques Rezende ; Maria Andreia Nunes ; Mário Eidi Sato ; Pedro Luis Ramos González ; Renata Faier Calegario ; RENATA SANTOS DE MENDONCA ; Renato Beozzo Bassanezi ; Valdenice Moreira Novelli
• Associated research grant(s): 22/11318-0 - Multi-user equipment granted within the thematic project 2019/25078-9: Ultramicrotome Leica UC7RT, AP.EMU
• Associated scholarship(s): 24/23284-9 - The role of orphan ORFs of the passionfruit green spot virus (Cilevirus passiflorae), BP.PD; 22/05043-9 - Solanum violifolium as a natural host for three different viruses transmitted by Brevipalpus mites: a model for the study of the interaction among these viruses, BP.IC; 21/09041-8 - Detection of passion fruit green spot virus in plant hosts and evaluation of the 5' end of its RNA2, BP.IC; 21/08146-0 - Analysis of methodologies for efficient use of RNAi technology applied to studies of Brevipalpus yothersi - the mite vector of citrus leprosis, BP.TT; 21/03113-7 - On the possibility that phoresis is responsible for the dispersion of Brevipalpus mites and the viruses they transmit, BP.IC

Abstract

Knowledge on the Citrus Leprosis pathosystem (CL), one of the most importante citrus diseases in the state of São Paulo, gained exponential increase in the last 20 years. Its main causal agente, Citrus Leprosis Virus (CiLV-C), transmitted by Brevipalpus mites, was thoroughly characterized and the management of CL was immensely helped by the epidemiological data yielded. In the wake of these advances, it was discovered that at least four other viruses are part of the Leprosis syndrome in citrus plants, and that many other viral diseases in plants as coffee, passion flower and many ornamentals are also caused by viruses transmitted by Brevipalpus in more thatn 40 plant species, naturally infected. Taxonomy, morphology, biology, genomics of Brevipalpus, and its relationship with transmitted viruses had significative advances. A remarkable achievement was that there are at least two completely different types of viruses, being transmitted by Brevipalpus (BTV), causing essentially similar localized symptoms belonging to the family Rhabdoviridae and Kitaviridae, suggesting a converging evolution. Most of these knowledges came from researches supported by FAPESP, which produced 105 articles, 10 technical publications, 1 book and 1 book chapter, 15 dissertations and thesis, and 264 abstracts presented in local and international meetings. The present proposal intends to maintain a continuity on these works, focusing: (a) in the use of new genomic/transcriptomic technologies applied onto the BTV/vector/host relationship; (b) a deepening on taxonomy and gene prospection of Brevipalpus; (c) the introduction of new strategies in the management of CL due to the changes of cultural technique in citrus orchards, after the recent introduction of HLB in the state of São Paulo. (AU)

Scientific publications

(13)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RAMOS-GONZALEZ, PEDRO L.; PONS, TIRSO; CHABI-JESUS, CAMILA; ARENA, GABRIELLA DIAS; FREITAS-ASTUA, JULIANA. oorly Conserved P15 Proteins of Cileviruses Retain Elements of Common Ancestry and Putative Functionality: A Theoretical Assessment on the Evolution of Cilevirus Genome. FRONTIERS IN PLANT SCIENCE, v. 12, NOV 5 2021. (19/02137-0, 19/25078-9, 17/50222-0, 20/15413-2)

RAMOS-GONZALEZ, PEDRO LUIS; KONDO, HIDEKI; MOROZOV, SERGEY; VASILAKIS, NIKOLAOS; VARSANI, ARVIND; CAO, MENGJI; FREITAS-ASTUA, JULIANA. Editorial: The Border Between Kitavirids and Nege-Like Viruses: Tracking the Evolutionary Pace of Plant- and Arthropod-Infecting Viruses. FRONTIERS IN PLANT SCIENCE, v. 13, p. 2-pg., 2022-05-24. (17/50334-3, 19/25078-9, 17/50222-0)

CHABI-JESUS, CAMILA; RAMOS-GONZALEZ, PEDRO LUIS; TASSI, ALINE DANIELE; ROSSETTO PEREIRA, LAURA; BASTIANEL, MARINES; LAU, DOUGLAS; CANALE, MARIA CRISTINA; HARAKAVA, RICARDO; NOVELLI, VALDENICE MOREIRA; KITAJIMA, ELLIOT WATANABE; et al. Citrus Bright Spot Virus: A New Dichorhavirus, Transmitted by Brevipalpus azores, Causing Citrus Leprosis Disease in Brazil. PLANTS-BASEL, v. 12, n. 6, p. 15-pg., 2023-03-01. (17/50222-0, 20/15413-2, 19/02137-0, 18/12252-8, 17/50334-3, 19/25078-9)

ELLIOT WATANABE KITAJIMA; ERICH YUKIO TEMPEL NAKASU; ALICE KAZUKO INOUE-NAGATA; RENATO BARBOSA SALAROLI; PEDRO LUIS RAMOS-GONZÁLEZ. Tomato fruit blotch virus cytopathology strengthens evolutionary links between plant blunerviruses and insect negeviruses. Scientia Agricola, v. 80, 2022-06-24. (19/25078-9)

CHABI-JESUS, CAMILA; RAMOS-GONZALEZ, PEDRO L.; POSTCLAM-BARRO, MATHEUS; FONTENELE, RAFAELA SALGADO; HARAKAVA, RICARDO; BASSANEZI, RENATO B.; MOREIRA, ALECIO S.; KITAJIMA, ELLIOT W.; VARSANI, ARVIND; FREITAS-ASTUA, JULIANA. Molecular Epidemiology of Citrus Leprosis Virus C: A New Viral Lineage and Phylodynamic of the Main Viral Subpopulations in the Americas. FRONTIERS IN MICROBIOLOGY, v. 12, APR 29 2021. (16/01960-6, 17/50334-3, 17/50222-0, 19/25078-9, 20/06378-9, 19/08186-2)

HESKETH, PATRICIA MAGNABOSCHI; SATO, MARIO EIDI. Acaricide resistance in predatory mites of the genus Euseius (Acari: Phytoseiidae) and predation capacity on Brevipalpus yothersi (Acari: Tenuipalpidae). CROP PROTECTION, v. 172, p. 8-pg., 2023-10-01. (17/50334-3, 19/25078-9)

RAMOS-GONZALEZ, PEDRO LUIS; ARENA, GABRIELLA DIAS; TASSI, ALINE DANIELE; CHABI-JESUS, CAMILA; KITAJIMA, ELLIOT WATANABE; FREITAS-ASTUA, JULIANA. Kitaviruses: A Window to Atypical Plant Viruses Causing Nonsystemic Diseases. ANNUAL REVIEW OF PHYTOPATHOLOGY, v. 61, p. 22-pg., 2023-01-01. (17/50222-0, 20/15413-2, 19/02137-0, 18/12252-8, 17/50334-3, 19/25078-9)

JOÃO PAULO RODRIGUES MARQUES; ELLIOT WATANABE KITAJIMA; BEATRIZ APPEZZATO-DA-GLÓRIA. Anatomy of stem lesions caused by Citrus leprosis. Rodriguésia, v. 75, 2024-04-29. (19/25078-9)

NASCIMENTO, RENATA S.; SOUZA, KAELEM S.; MELO, ELISANGELA A. S. F.; TASSI, ALINE D.; CASTRO, ELIZEU B.; NAVIA, DENISE; DE MENDONCA, RENATA S.; OCHOA, RONALD; OLIVEIRA, ANIBAL R.. Flat mites (Tenuipalpidae) from Bahia state, Northeastern Brazil- a checklist including new records and an illustrated key to species. ACAROLOGIA, v. N/A, p. 19-pg., 2023-05-24. (19/25078-9, 18/12252-8)

NUNES, MARIA ANDREIA; FERREIRA, LETICIA MARIA; ROGERIO, LUANA APARECIDA; KITAJIMA, ELLIOT WATANABE; NOVELLI, VALDENICE MOREIRA. Infection by coffee ringspot virus is identified for the first time in succulent plants. JOURNAL OF PLANT PATHOLOGY, v. 104, n. 2, p. 5-pg., 2022-03-23. (14/08458-9, 19/25078-9, 15/23464-8)

DE LILLO, ENRICO; FREITAS-ASTUA, JULIANA; KITAJIMA, ELLIOT WATANABE; RAMOS-GONZALEZ, PEDRO LUIS; SIMONI, SAURO; TASSI, ALINE DANIELE; VALENZANO, DOMENICO. Phytophagous mites transmitting plant viruses: update and perspectives. ENTOMOLOGIA GENERALIS, v. 41, n. 5, p. 439-462, 2021. (14/08458-9, 17/50222-0, 18/12252-8, 19/25078-9)

KITAJIMA, ELLIOT WATANABE; TEMPEL NAKASU, ERICH YUKIO; INOUE-NAGATA, ALICE KAZUKO; SALAROLI, RENATO BARBOSA; RAMOS-GONZALEZ, PEDRO LUIS. Tomato fruit blotch virus cytopathology strengthens evolutionary links between plant blunerviruses and insect negeviruses. CIENTIA AGRICOL, v. 80, p. 10-pg., 2023-01-01. (19/25078-9)

TASSI, ALINE DANIELE; RAMOS-GONZALEZ, PEDRO LUIS; SINICO, THAIS ELISE; KITAJIMA, ELLIOT WATANABE; FREITAS-ASTUA, JULIANA. Circulative Transmission of Cileviruses in Brevipalpus Mites May Involve the Paracellular Movement of Virions. FRONTIERS IN MICROBIOLOGY, v. 13, p. 11-pg., 2022-04-06. (17/50334-3, 19/25078-9, 18/12252-8, 17/50222-0)