Study of brain regions and neural mechanisms involved with respiratory responses in situations of hypercapnia during sleep and wakefulness

• Grant number: 19/17693-5
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2021
• End date: January 31, 2023
• Field of knowledge: Biological Sciences - Physiology - General Physiology
• Principal Investigator: Mirela Barros Dias
• Grantee: Mirela Barros Dias
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated researchers: Daniel Kent Mulkey

Abstract

Central chemoreception is a fundamental homeostatic mechanism and the dysfunction in its regulation, especially during sleep, has been related to several sleep-related respiratory diseases, which are present in an increasingly prevalent way in the current population. Evidence suggests that throughout the sleep-wake cycle there is a variation of central chemoreflex, as well as the predominance of the chemosensitive sites and neurochemical mechanisms involved, however such mechanisms are still little known. Thus, the present project proposes to study neurotransmitters (orexins, melatonin, MCH) and regions of the central nervous system classically involved in the control of the sleep-wake cycle such as the tegmental nucleus pontine peduncle (PPTg), or involved with respiratory disorders during sleep as is the case with the kölliker-fuse (KF) nucleus, which share functions in respiratory control and, possibly, have relevance in the control of the hypercapnic central chemoreflex. We will investigate the possible role of these neurotransmitters and brain regions in the modulation of respiratory control during activation of the central chemoreflex, during wakefulness and sleep. To do so, neuroanatomical and functional techniques will be used, including immunohistochemistry, microinjections, central microdialysis, pulmonary ventilation recordings in unanesthetized rats, EEG, EMG, body temperature as well as electrophysiological experiments, with patch-clamp technique. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DIAS, MIRELA B.; RODRIGUES, LAISA T. C.; GARGAGLIONI, LUCIANE H.. Melanin -Concentrating Hormone (MCH) Decreases the Hypercapnic Ventilatory Response by Acting in the Lateral Hypothalamic Area (LHA). FASEB JOURNAL, v. 36, p. 1-pg., 2022-01-01. (19/17693-5, 18/01781-0)

DA SILVA, ELIANDRA N.; HORTA-JUNIOR, JOSE DE ANCHIETA C.; GARGAGLIONI, LUCIANE H.; DIAS, MIRELA B.. CO2 exposure enhances Fos expression in hypothalamic neurons in rats during the light and dark phases of the diurnal cycle. Brain Structure & Function, v. 227, n. 8, p. 13-pg., 2022-09-15. (13/04216-8, 19/17693-5)

DA SILVA, ELIANDRA; DE ANCHIETA E HORTA-JUNIOR, JOSE; DIAS, MIRELA. Orexinergic signaling in the commissural nucleus of solitary tract participates in the hypercapnic ventilatory response during wakefulness in light phase of the diurnal cycle. PHYSIOLOGY, v. 38, p. 2-pg., 2023-05-01. (19/17693-5)

RODRIGUES, LAISA T. C.; PATRONE, LUIS GUSTAVO A.; GARGAGLIONI, LUCIANE H.; DIAS, MIRELA B.. Melanin-concentrating hormone regulates the hypercapnic chemoreflex by acting in the lateral hypothalamic area. Experimental Physiology, v. 107, n. 11, p. 14-pg., 2022-08-22. (19/17693-5, 18/01781-0)