Qualitative and quantitative study of the extracellular matrix (MEC) and immunohistochemical study of subpopulations of skin macrophages in patients with American cutaneous Leishmaniasis (ATL) and Hansen's disease

• Grant number: 20/05388-0
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2021
• End date: January 31, 2024
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Principal Investigator: Marcia Dalastra Laurenti
• Grantee: Marcia Dalastra Laurenti
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Carla Pagliari ; Claudia Maria de Castro Gomes ; David Michael Mosser ; Fernando Tobias Silveira ; Marília Brasil Xavier ; Wagner Luiz Tafuri

Abstract

In both Hansen's disease and American Tegumentary Leishmaniasis (ATL) there is two distinct (extreme) clinical status characterized by an intense chronic inflammatory cellular exudate with low parasite tissue load, as follow: Tuberculoid Hansen's disease (HT) and mucocutaneous leishmaniasis (CML). In general, even considering the particularities of each disease, it can be said that both show chronic granulomatous inflammatory response with a predominance of macrophages, which are differentiated into epithelioid macrophages, surrounded by plasma cells and lymphocytes (Epithelioid granuloma formation). Besides, the granuloma, called complete (tuberculoid granuloma), must also contain a central caseous necrosis area. In the hyporeactive extreme are Diffuse Anergic Cutaneous Leishmaniasis (LCAD) and Lepromatous Hanseniase (HL). Although both diseases have particular histological aspects, in both, there is a paucity of lymphocytes in the chronic inflammatory infiltrate, despite the intense parasite tissue load. It is observed a presence of numerous macrophages crowded with parasites forming clusters called "Virchow's granulomas". Therefore, the aim of this project is to characterize macrophages in the clinical spectrum of ATL and Hansen's disease lesions, but in particular looking for elucidating the differentiation of M2 macrophages related to interstitial changes, especially collagenogenesis (fibrosis). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

REGO, FELIPE D.; CARDOSO, CAMILA D. A.; MOREIRA, PAULO OTAVIO L.; NOGUEIRA, PAULA M.; ARAUJO, MARCIO S.; BORGES, VALERIA MATOS; LAURENTI, MARCIA D.; BARTHOLOMEU, DANIELLA C.; REIS, ALEXANDRE B.; MONTE-NETO, RUBENS L. D.; et al. Leishmania amazonensis from distinct clinical forms/hosts has polymorphisms in Lipophosphoglycans, displays variations in immunomodulatory properties and, susceptibility to antileishmanial drugs. Cell Biology International, v. 46, n. 11, p. 12-pg., 2022-08-23. (20/05388-0, 21/01243-0)