OneBR (One Health Brazilian Resistance): integrated genomic database for surveillance, diagnosis, management and treatment of antimicrobial resistance in the human-animal-environment interface, in Brazil

• Grant number: 20/08224-9
• Support Opportunities: Regular Research Grants
• Duration: December 01, 2020 - August 31, 2023
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Nilton Erbet Lincopan Huenuman
• Grantee: Nilton Erbet Lincopan Huenuman
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The emergence and spread of drug-resistant bacteria has become a global public health problem that has compromised the effective treatment of an increasing range of infections that affect humans and other animals, also impacting environmental health, and, therefore be addressed as a One Health problem. In this regard, the high prevalence and endemicity of bacterial species such as Escherichia coli, Klebsiella pneumoniae, Salmonella enterica and Pseudomonas aeruginosa, in healthcare-related infections (HAI), it has been supported by the well-determined combination of virulence factors (viruloma) and the presence of antibiotic, disinfectant and heavy metal resistance genes (resistoma). In this context, the genetic determinants of resistance mobilized by plasmids stand out, with emphasis on the production of broad-spectrum beta-lactamases (ESBLs), carbapenemases (KPC, NDM) and transferable resistance to colistin (MCR). As an aggravating factor, in Brazil, the dissemination of these genetic determinants in these pathogens has occurred dynamically at the human-environment-animal interface, with a rapid adaptation of high risk clones, such as E. coli ST10, ST38, ST131 , ST224, and ST648; K. pneumoniae belonging to CC258; and P. aeruginosa ST277. Thus, using a genomic approach, the present study aims to clarify aspects related to the resistoma, viruloma, mobiloma and plasmidoma of pandemic lineages of clinically important Gram-negative bacteria from different hosts and ecosystems. To this end, an integrated genomics database with easy access, named "OneBR" (One Health Brazilian Resistance), will be created, which will enable the surveillance, diagnosis and treatment of antimicrobial resistance within the One Health concept. The benefit of this study will result from the availability of genomic data (big data) on a free platform easily accessible by professionals and researchers in the area of human, animal and environmental health, and a priori will allow to elucidate genetic aspects of adaptation, resistance and virulence of these pathogens considered of critical priority by the world health organization (WHO), circulating in the human-environment-animal interface, in Brazil. (AU)

Scientific publications

(15)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GAETA, NATALIA C.; DE CARVALHO, DANIEL U.; FONTANA, HERRISON; SANO, ELDER; MOURA, QUEZIA; FUGA, BRUNA; MUNOZ, PATRICIO MONTECINOS; GREGORY, LILIAN; LINCOPAN, NILTON. Genomic features of a multidrug-resistant and mercury-tolerant environmental Escherichia coli recovered after a mining dam disaster in South America. Science of The Total Environment, v. 823, p. 9-pg., 2022-06-01. (16/23204-9, 20/08224-9)

ESPOSITO, FERNANDA; CARDOSO, BRENDA; SELLERA, FABIO P.; SANO, ELDER; FUENTES-CASTILLO, DANNY; FONTANA, HERRISON; FUGA, BRUNA; MOURA, QUEZIA; SATO, MARIA I. Z.; BRANDAO, CARLOS J.; et al. Expansion of healthcare-associated hypervirulent KPC-2-producing Klebsiella pneumoniae ST11/KL64 beyond hospital settings. ONE HEALTH, v. 17, p. 6-pg., 2023-12-01. (19/15578-4, 20/08224-9)

VASQUEZ-PONCE, FELIPE; BISPO, JESSICA; BECERRA, JOHANA; FONTANA, HERRISON; PARIONA, JESUS G. M.; ESPOSITO, FERNANDA; FUGA, BRUNA; OLIVEIRA, FLAVIO A.; BRUNETTI, FLORENCIA; POWER, PABLO; et al. Emergence of KPC-113 and KPC-114 variants in ceftazidime-avibactam-resistant Klebsiella pneumoniae belonging to high-risk clones ST11 and ST16 in South America. MICROBIOLOGY SPECTRUM, v. N/A, p. 9-pg., 2023-09-06. (19/15578-4, 20/08224-9)

BECERRA, JOHANA; ARAUJO, GABRIEL G.; VASQUEZ-PONCE, FELIPE; LAMADRID-FERIS, FARIDE; LINCOPAN, NILTON. ACT-107, a novel variant of AmpC β-lactamase from Enterobacter huaxiensis isolated from Neotropical leaf frog (Phyllomedusa distincta) inhabiting the Brazilian Atlantic Forest. JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, v. 33, p. 7-pg., 2023-06-08. (20/08224-9, 14/16320-7, 18/21076-9)

ESPOSITO, FERNANDA; CARDOSO, BRENDA; FONTANA, HERRISON; FUGA, BRUNA; CARDENAS-ARIAS, ADRIANA; MOURA, QUEZIA; FUENTES-CASTILLO, DANNY; LINCOPAN, NILTON. Genomic Analysis of Carbapenem-Resistant Pseudomonas aeruginosa Isolated From Urban Rivers Confirms Spread of Clone Sequence Type 277 Carrying Broad Resistome and Virulome Beyond the Hospital. FRONTIERS IN MICROBIOLOGY, v. 12, SEP 3 2021. (20/08224-9, 19/15578-4)

FUGA, BRUNA; SELLERA, FABIO P.; CERDEIRA, LOUISE; ESPOSITO, FERNANDA; CARDOSO, BRENDA; FONTANA, HERRISON; MOURA, QUEZIA; CARDENAS-ARIAS, ADRIANA; SANO, ELDER; RIBAS, ROSINEIDE M.; et al. WHO Critical Priority Escherichia coli as One Health Challenge for a Post-Pandemic Scenario: Genomic Surveillance and Analysis of Current Trends in Brazil. MICROBIOLOGY SPECTRUM, v. 10, n. 2, p. 18-pg., 2022-04-01. (19/15578-4, 20/08224-9)

SELLERA, FABIO P.; DA SILVA, LUCIANO C. B. A.; LINCOPAN, NILTON. Rapid spread of critical priority carbapenemase-producing pathogens in companion animals: a One Health challenge for a post-pandemic world. Journal of Antimicrobial Chemotherapy, v. 76, n. 9, p. 2225-2229, SEP 2021. (20/08224-9)

SELLERA, FABIO P.; FUENTES-CASTILLO, DANNY; FUGA, BRUNA; GOLDBERG, DAPHNE W.; KOLESNIKOVAS, CRISTIANE K. M.; LINCOPAN, NILTON. New Delhi metallo-β-lactamase-1-producing Citrobacter portucalensis belonging to the novel ST264 causing fatal sepsis in a vulnerable migratory sea turtle. ONE HEALTH, v. 17, p. 5-pg., 2023-06-24. (20/08224-9)

SANO, ELDER; FONTANA, HERRISON; ESPOSITO, FERNANDA; CARDOSO, BRENDA; FUGA, BRUNA; COSTA, GLADYSTON C. V.; BOSQUEIRO, TATIANA C. M.; SINHORINI, JULIANA A.; ORICO, LILIAN D.; DE MASI, EDUARDO; et al. Genomic analysis of fluoroquinolone-resistant Leclercia adecarboxylata carrying the ISKpn19- orf- qnrS1- Delta IS3-blaLAP-2 module in a synanthropic pigeon, Brazil. JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, v. 33, p. 4-pg., 2023-05-14. (20/08224-9)

BUERIS, VANESSA; SELLERA, FABIO P.; FUGA, BRUNA; SANO, ELDER; CARVALHO, MARCELO P. N.; COUTO, SAMUEL C. F.; MOURA, QUEZIA; LINCOPAN, NILTON. Convergence of virulence and resistance in international clones of WHO critical priority enterobacterales isolated from Marine Bivalves. SCIENTIFIC REPORTS, v. 12, n. 1, p. 12-pg., 2022-04-05. (20/08224-9)

SACRAMENTO, ANDREY G.; FUGA, BRUNA; MONTE, DANIEL F. M.; CARDOSO, BRENDA; ESPOSITO, FERNANDA; DOLABELLA, SILVIO S.; BARBOSA, ANA A. T.; ZANELLA, ROSEMEIRE C.; CORTOPASSI, SILVIA R. G.; DA SILVA, LUCIANO C. B. A.; et al. Genomic features of mecA-positive methicillin-resistant Mammaliicoccus sciuri causing fatal infections in pets admitted to a veterinary intensive care unit. Microbial Pathogenesis, v. 171, p. 6-pg., 2022-10-01. (20/08224-9)

SELLERA, FABIO P.; FERNANDES, MIRIAM R.; FUGA, BRUNA; FONTANA, HERRISON; VASQUEZ-PONCE, FELIPE; GOLDBERG, DAPHNE W.; MONTE, DANIEL F.; RODRIGUES, LARISSA; CARDENAS-ARIAS, ADRIANA R.; LOPES, RALF; et al. Phylogeographical Landscape of Citrobacter portucalensis Carrying Clinically Relevant Resistomes. MICROBIOLOGY SPECTRUM, v. 10, n. 2, p. 7-pg., 2022-04-01. (19/15578-4, 20/08224-9, 15/13527-2)

FONTANA, HERRISON; CARDOSO, BRENDA; ESPOSITO, FERNANDA; DE LIMA, ALINE VALERIO; MELLO SAMPAIO, JORGE LUIZ; LINCOPAN, NILTON. mall IncQ1 Plasmid Encoding KPC-2 Expands to Invasive Nontyphoidal Salmonell. Antimicrobial Agents and Chemotherapy, v. 65, n. 11, OCT 2021. (20/08224-9)

VASQUEZ-PONCE, FELIPE; DANTAS, KARINE; BECERRA, JOHANA; MELOCCO, GREGORY; ESPOSITO, FERNANDA; CARDOSO, BRENDA; RODRIGUES, LARISSA; LIMA, KEILA; DE LIMA, ALINE, V; SELLERA, FABIO P.; et al. Detecting KPC-2 and NDM-1 Coexpression in Klebsiella pneumoniae Complex from Human and Animal Hosts in South America. MICROBIOLOGY SPECTRUM, v. N/A, p. 12-pg., 2022-08-18. (19/15578-4, 20/08224-9)

DALAZEN, GISLAINE; FUENTES-CASTILLO, DANNY; PEDROSO, LUIZ G.; FONTANA, HERRISON; SANO, ELDER; CARDOSO, BRENDA; ESPOSITO, FERNANDA; MOURA, QUEZIA; MATINATA, BIANCA S.; SILVEIRA, LUIZ F.; et al. CTX-M-producing Escherichia coli ST602 carrying a wide resistome in South American wild birds: Another pandemic clone of One Health concern. ONE HEALTH, v. 17, p. 7-pg., 2023-12-01. (19/15578-4, 20/08224-9)