Multi-user Equipment (MUE) approved in the grant 20/07611-9: Real Time Quantitative PCR (qPCR) Instrument

• Grant number: 21/03402-9
• Support Opportunities: Multi-user Equipment Program
• Start date: June 01, 2021
• End date: May 31, 2028
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Principal Investigator: Gabriel Madoglio Favara
• Grantee: Gabriel Madoglio Favara
• Host Institution: Faculdade de Engenharia (FEIS). Universidade Estadual Paulista (UNESP). Campus de Ilha Solteira. Ilha Solteira , SP, Brazil
• Associated research grant: 20/07611-9 - Development of a joint platform for disease epidemics monitoring and fungicide resistance detection to foster a smart program for management of wheat blast in Paraná and São Paulo states, AP.R
• EMU web page: Página do Equipamento Multiusuário não informada
• Type of equipment: Caracterização e Análises de Amostras - Proteínas/Ácidos nuclêicos - PCR
• Manufacturer: Fabricante não informado
• Model: Modelo não informado

Abstract

Some plant diseases are becoming very difficult to control due to lack of host resistance cultivars and limited availability of fungicides for crop protection. Fungicide resistance, tighter regulations and a slowing pipeline of new products are reducing the range of available chemical classes. This leads to greater dependence on fewer active ingredients with fungicide modes of action, and subsequently increases the selective pressure for further cases of resistance. The limited availability of effective crop protection products, coupled with lack of genetic resistance in major crop varieties, is making key pathogens increasingly difficult to control. In order to increase the shelf life of new and currently available fungicide actives, "evolution-smart" (i.e., guided) integrated pest management strategies are needed. Strategies based on different dose rates, alternations and mixtures of fungicides have been advocated to reduce the selection of resistance. This project will focus on the plant pathogen Pyricularia oryzae Triticum lineage (PoTl), the causal agent of wheat blast, an important fungal disease in Brazil that is very difficult to control, with several groups of fungicides (e.g., sterol demethylase, quinone outside and succinate dehydrogenase inhibitors) became (or having become) ineffective. In addition, onset of disease epidemics is poorly understood and, therefore, appropriate anti-resistance strategies and optimal disease control cannot be achieved. To improve wheat blast control, a better understanding of the disease epidemiology, the fungicide sensitivity/resistance status and new disease management strategies are needed. In this project we will focus on all three aspects using the latest spore trapping technology, fungicide pheno- and genotyping sensitivity/resistance assays enabling quantitative measurement of pathogen levels and detection of fungicide resistant alleles, in combination with disease forecasting, are needed. We will develop real-time disease surveillance, using automated spore trapping with pathogen DNA detection. We will then develop molecular diagnostics for rapid, high-throughput monitoring of fungicide resistance. These tools are generic and can be applied to study evolution and selection of fungicide resistance mechanisms in other pathogens that are important to Brazil. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

OLIVEIRA, TAMIRIS Y. K.; SILVA, TATIANE C.; MOREIRA, SILVINO I.; CHRISTIANO JR, FELIX S.; GASPAROTO, MARIA C. G.; FRAAIJE, BART A.; CERESINI, PAULO C.. Evidence of Resistance to QoI Fungicides in Contemporary Populations of Mycosphaerella fijiensis, M. musicola and M. thailandica from Banana Plantations in Southeastern Brazil. AGRONOMY-BASEL, v. 12, n. 12, p. 19-pg., 2022-12-01. (17/50456-1, 18/21197-0, 19/12509-1, 20/07611-9, 21/03402-9)

SILVA, TATIANE CARLA; MOREIRA, SILVINO INTRA; ASSIS JR, FABIO GOMES; CAMPOS VICENTINI, SAMARA NUNES; SILVA, ABIMAEL GOMES; KITAYAMA DE OLIVEIRA, TAMIRIS YOSHIE; CHRISTIANO JR, FELIX SEBASTIAO; DE PAIVA CUSTODIO, ADRIANO AUGUSTO; LEITE JR, RUI PEREIRA; DE GODOY GASPAROTO, MARIA CANDIDA; et al. An Accurate, Affordable, and Precise Resazurin-Based Digital Imaging Colorimetric Assay for the Assessment of Fungicide Sensitivity Status of Fungal Populations. AGRONOMY-BASEL, v. 13, n. 2, p. 19-pg., 2023-02-01. (20/07611-9, 21/03402-9, 18/21197-0, 19/12509-1, 17/50456-1)

CAMPOS VICENTINI, SAMARA NUNES; HAWKINS, NICHOLA J.; KING, KEVIN M.; MOREIRA, SILVINO INTRA; DE PAIVA CUSTODIO, ADRIANO AUGUSTO; LEITE JUNIOR, RUI PEREIRA; PORTALANZA, DIEGO; GARCES-FIALLOS, FELIPE RAFAEL; KRUG, LOANE DANTAS; WEST, JONATHAN S.; et al. Aerobiology of the Wheat Blast Pathogen: Inoculum Monitoring and Detection of Fungicide Resistance Alleles. AGRONOMY-BASEL, v. 13, n. 5, p. 17-pg., 2023-04-27. (22/09787-2, 20/07611-9, 21/03402-9, 18/21197-0, 19/12509-1, 17/50456-1)